B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model

Interstitial cystitis (IC) is a chronic inflammatory disease characterized by bladder pain and increased urinary frequency. Although the C57BL/6J (B6) and FVB/NJ (FVB) mouse strains are commonly used as animal models for studies involving the urinary system, few reports have compared their lower uri...

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Autores principales:	Ching-Hao Chen, Chun-Hou Liao, Kuo-Chiang Chen, Kuan-Lin Wang, Xiao-Wen Tseng, Wei-Kung Tsai, Han-Sun Chiang, Yi-No Wu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	interstitial cystitis lipopolysaccharide FVB mouse strain B6 mouse strain cytometry Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/f019c4bd5b2c482aa2bbb04a3a161a17
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spelling	oai:doaj.org-article:f019c4bd5b2c482aa2bbb04a3a161a172021-11-11T17:27:03ZB6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model10.3390/ijms2221120531422-00671661-6596https://doaj.org/article/f019c4bd5b2c482aa2bbb04a3a161a172021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12053https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Interstitial cystitis (IC) is a chronic inflammatory disease characterized by bladder pain and increased urinary frequency. Although the C57BL/6J (B6) and FVB/NJ (FVB) mouse strains are commonly used as animal models for studies involving the urinary system, few reports have compared their lower urinary tract anatomy, despite the importance of such data. Our study aimed to characterize bladder function changes in FVB and B6 mouse strains with lipopolysaccharide (LPS)-induced IC, to understand mouse model-based bladder research. The bladder function parameters were measured by cystometrogram. Histological assay was examined by hematoxylin and eosin stain, Masson’s trichrome stain, and immunofluorescence staining. Results indicated that the two strains in the control group exhibited different bladder structures and functions, with significant anatomical differences, including a larger bladder size in the FVB than in the B6 strain. Furthermore, cystometry tests revealed differences in bladder function pressure. LPS-treated B6 mice presented significant changes in peak pressure, with decreased intercontraction intervals; these results were similar to symptoms of IC in humans. Each strain displayed distinct characteristics, emphasizing the care required in choosing the appropriate strain for bladder-model studies. The results suggested that the B6 mouse strain is more suitable for IC models.Ching-Hao ChenChun-Hou LiaoKuo-Chiang ChenKuan-Lin WangXiao-Wen TsengWei-Kung TsaiHan-Sun ChiangYi-No WuMDPI AGarticleinterstitial cystitislipopolysaccharideFVB mouse strainB6 mouse straincytometryBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12053, p 12053 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	interstitial cystitis lipopolysaccharide FVB mouse strain B6 mouse strain cytometry Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	interstitial cystitis lipopolysaccharide FVB mouse strain B6 mouse strain cytometry Biology (General) QH301-705.5 Chemistry QD1-999 Ching-Hao Chen Chun-Hou Liao Kuo-Chiang Chen Kuan-Lin Wang Xiao-Wen Tseng Wei-Kung Tsai Han-Sun Chiang Yi-No Wu B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model
description	Interstitial cystitis (IC) is a chronic inflammatory disease characterized by bladder pain and increased urinary frequency. Although the C57BL/6J (B6) and FVB/NJ (FVB) mouse strains are commonly used as animal models for studies involving the urinary system, few reports have compared their lower urinary tract anatomy, despite the importance of such data. Our study aimed to characterize bladder function changes in FVB and B6 mouse strains with lipopolysaccharide (LPS)-induced IC, to understand mouse model-based bladder research. The bladder function parameters were measured by cystometrogram. Histological assay was examined by hematoxylin and eosin stain, Masson’s trichrome stain, and immunofluorescence staining. Results indicated that the two strains in the control group exhibited different bladder structures and functions, with significant anatomical differences, including a larger bladder size in the FVB than in the B6 strain. Furthermore, cystometry tests revealed differences in bladder function pressure. LPS-treated B6 mice presented significant changes in peak pressure, with decreased intercontraction intervals; these results were similar to symptoms of IC in humans. Each strain displayed distinct characteristics, emphasizing the care required in choosing the appropriate strain for bladder-model studies. The results suggested that the B6 mouse strain is more suitable for IC models.
format	article
author	Ching-Hao Chen Chun-Hou Liao Kuo-Chiang Chen Kuan-Lin Wang Xiao-Wen Tseng Wei-Kung Tsai Han-Sun Chiang Yi-No Wu
author_facet	Ching-Hao Chen Chun-Hou Liao Kuo-Chiang Chen Kuan-Lin Wang Xiao-Wen Tseng Wei-Kung Tsai Han-Sun Chiang Yi-No Wu
author_sort	Ching-Hao Chen
title	B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model
title_short	B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model
title_full	B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model
title_fullStr	B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model
title_full_unstemmed	B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model
title_sort	b6 mouse strain: the best fit for lps-induced interstitial cystitis model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f019c4bd5b2c482aa2bbb04a3a161a17
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B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model

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