Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions

<h4>Objective</h4> The oxygen concentration within cancer tissue is known to be low, but is expected to increase rapidly when oxygen is supplied by angiogenesis and hematogenous metastasis, suggesting that rapid increases in oxygen levels might influence cancer cell physiology. Therefore...

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Autores principales:	Yuta Shinohara, Jumpei Washio, Yuri Kobayashi, Yuki Abiko, Keiichi Sasaki, Nobuhiro Takahashi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f02027260c4d417db4e9b8b723b8a416
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spelling	oai:doaj.org-article:f02027260c4d417db4e9b8b723b8a4162021-11-04T06:07:12ZHypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions1932-6203https://doaj.org/article/f02027260c4d417db4e9b8b723b8a4162021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535375/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4> The oxygen concentration within cancer tissue is known to be low, but is expected to increase rapidly when oxygen is supplied by angiogenesis and hematogenous metastasis, suggesting that rapid increases in oxygen levels might influence cancer cell physiology. Therefore, we investigated the effects of oxygen concentration fluctuations on the glucose metabolism of cancer cells. <h4>Methods</h4> The glucose metabolism of oral squamous cell carcinoma (HSC-2 and HSC-3) and normal epithelial (HaCaT) cells cultured under normoxic (21% oxygen) or hypoxic (1% oxygen) conditions was measured using a pH-stat system under normoxic or hypoxic conditions. The acidic end-products and reactive oxygen species (ROS) generated by glucose metabolism were also measured. <h4>Results</h4> Under normoxic conditions, the metabolic activity of hypoxically cultured cancer cells was significantly increased, and the production of acids other than lactate was upregulated, while the normal cells did not respond to rapid increases in oxygen levels. ROS production was higher in normoxic conditions in all cells, especially the hypoxically cultured HSC-3 cells. <h4>Conclusions</h4> Rapid increases in oxygen levels might enhance the glucose metabolism of hypoxically cultured cancer cells by mainly activating the TCA cycle and electron transport system, which might activate cancer cells through the ATP and ROS generation.Yuta ShinoharaJumpei WashioYuri KobayashiYuki AbikoKeiichi SasakiNobuhiro TakahashiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuta Shinohara Jumpei Washio Yuri Kobayashi Yuki Abiko Keiichi Sasaki Nobuhiro Takahashi Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions
description	<h4>Objective</h4> The oxygen concentration within cancer tissue is known to be low, but is expected to increase rapidly when oxygen is supplied by angiogenesis and hematogenous metastasis, suggesting that rapid increases in oxygen levels might influence cancer cell physiology. Therefore, we investigated the effects of oxygen concentration fluctuations on the glucose metabolism of cancer cells. <h4>Methods</h4> The glucose metabolism of oral squamous cell carcinoma (HSC-2 and HSC-3) and normal epithelial (HaCaT) cells cultured under normoxic (21% oxygen) or hypoxic (1% oxygen) conditions was measured using a pH-stat system under normoxic or hypoxic conditions. The acidic end-products and reactive oxygen species (ROS) generated by glucose metabolism were also measured. <h4>Results</h4> Under normoxic conditions, the metabolic activity of hypoxically cultured cancer cells was significantly increased, and the production of acids other than lactate was upregulated, while the normal cells did not respond to rapid increases in oxygen levels. ROS production was higher in normoxic conditions in all cells, especially the hypoxically cultured HSC-3 cells. <h4>Conclusions</h4> Rapid increases in oxygen levels might enhance the glucose metabolism of hypoxically cultured cancer cells by mainly activating the TCA cycle and electron transport system, which might activate cancer cells through the ATP and ROS generation.
format	article
author	Yuta Shinohara Jumpei Washio Yuri Kobayashi Yuki Abiko Keiichi Sasaki Nobuhiro Takahashi
author_facet	Yuta Shinohara Jumpei Washio Yuri Kobayashi Yuki Abiko Keiichi Sasaki Nobuhiro Takahashi
author_sort	Yuta Shinohara
title	Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions
title_short	Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions
title_full	Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions
title_fullStr	Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions
title_full_unstemmed	Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions
title_sort	hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f02027260c4d417db4e9b8b723b8a416
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Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions

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