TatD DNases Contribute to Biofilm Formation and Virulence in Trueperella pyogenes

TatD DNases are conserved proteins in a variety of organisms and are considered potential virulence factors in Plasmodium falciparum and Streptococcus pneumoniae. However, the function of TatD DNases has not yet been determined in Trueperella pyogenes, which causes various infections in animals and...

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Autores principales:	Zehui Zhang, Yinfeng Liang, Lihui Yu, Menghan Chen, Yuru Guo, Zhiruo Kang, Chenghu Qu, Chunlian Tian, Dexian Zhang, Mingchun Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Trueperella pyogenes TatD DNases DNA hydrolysis biofilm virulence Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f0248b0e80e049029dbd57f3a6fc0563
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spelling	oai:doaj.org-article:f0248b0e80e049029dbd57f3a6fc05632021-11-15T06:43:26ZTatD DNases Contribute to Biofilm Formation and Virulence in Trueperella pyogenes1664-302X10.3389/fmicb.2021.758465https://doaj.org/article/f0248b0e80e049029dbd57f3a6fc05632021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.758465/fullhttps://doaj.org/toc/1664-302XTatD DNases are conserved proteins in a variety of organisms and are considered potential virulence factors in Plasmodium falciparum and Streptococcus pneumoniae. However, the function of TatD DNases has not yet been determined in Trueperella pyogenes, which causes various infections in animals and leads to economic losses. In this study, we describe the roles of TatD DNases in T. pyogenes (TpTatDs). A bioinformatics analysis was performed to investigate the sequence characteristics of TpTatDs, and then the ability of recombinant TatD proteins to hydrolyze DNA was determined in the presence of divalent cations. Moreover, we constructed tatD-deficient mutants. The biofilms formed by the wild-type and mutant strains were observed under a microscope. The mortality and bacterial load in the spleen of mice infected with the wild-type strain and tatD-deficient mutants were determined to obtain insights into the role of TatDs in the virulence of T. pyogenes. Two TatD DNases were identified in T. pyogenes. They were Mg2+-dependent DNases and exhibited DNA endonuclease activity. Compared with those formed by the parental strain, biofilms formed by mutants showed a significantly reduced thickness and biomass. Moreover, mutants produced a lower bacterial load in the spleen of mice and compromised virulence. Our data indicated that TatD DNases in T. pyogenes are involved in biofilm formation and required for virulence during infections.Zehui ZhangYinfeng LiangLihui YuMenghan ChenYuru GuoZhiruo KangChenghu QuChunlian TianDexian ZhangMingchun LiuFrontiers Media S.A.articleTrueperella pyogenesTatD DNasesDNA hydrolysisbiofilmvirulenceMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Trueperella pyogenes TatD DNases DNA hydrolysis biofilm virulence Microbiology QR1-502
spellingShingle	Trueperella pyogenes TatD DNases DNA hydrolysis biofilm virulence Microbiology QR1-502 Zehui Zhang Yinfeng Liang Lihui Yu Menghan Chen Yuru Guo Zhiruo Kang Chenghu Qu Chunlian Tian Dexian Zhang Mingchun Liu TatD DNases Contribute to Biofilm Formation and Virulence in Trueperella pyogenes
description	TatD DNases are conserved proteins in a variety of organisms and are considered potential virulence factors in Plasmodium falciparum and Streptococcus pneumoniae. However, the function of TatD DNases has not yet been determined in Trueperella pyogenes, which causes various infections in animals and leads to economic losses. In this study, we describe the roles of TatD DNases in T. pyogenes (TpTatDs). A bioinformatics analysis was performed to investigate the sequence characteristics of TpTatDs, and then the ability of recombinant TatD proteins to hydrolyze DNA was determined in the presence of divalent cations. Moreover, we constructed tatD-deficient mutants. The biofilms formed by the wild-type and mutant strains were observed under a microscope. The mortality and bacterial load in the spleen of mice infected with the wild-type strain and tatD-deficient mutants were determined to obtain insights into the role of TatDs in the virulence of T. pyogenes. Two TatD DNases were identified in T. pyogenes. They were Mg2+-dependent DNases and exhibited DNA endonuclease activity. Compared with those formed by the parental strain, biofilms formed by mutants showed a significantly reduced thickness and biomass. Moreover, mutants produced a lower bacterial load in the spleen of mice and compromised virulence. Our data indicated that TatD DNases in T. pyogenes are involved in biofilm formation and required for virulence during infections.
format	article
author	Zehui Zhang Yinfeng Liang Lihui Yu Menghan Chen Yuru Guo Zhiruo Kang Chenghu Qu Chunlian Tian Dexian Zhang Mingchun Liu
author_facet	Zehui Zhang Yinfeng Liang Lihui Yu Menghan Chen Yuru Guo Zhiruo Kang Chenghu Qu Chunlian Tian Dexian Zhang Mingchun Liu
author_sort	Zehui Zhang
title	TatD DNases Contribute to Biofilm Formation and Virulence in Trueperella pyogenes
title_short	TatD DNases Contribute to Biofilm Formation and Virulence in Trueperella pyogenes
title_full	TatD DNases Contribute to Biofilm Formation and Virulence in Trueperella pyogenes
title_fullStr	TatD DNases Contribute to Biofilm Formation and Virulence in Trueperella pyogenes
title_full_unstemmed	TatD DNases Contribute to Biofilm Formation and Virulence in Trueperella pyogenes
title_sort	tatd dnases contribute to biofilm formation and virulence in trueperella pyogenes
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f0248b0e80e049029dbd57f3a6fc0563
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_version_	1718428591765585920

TatD DNases Contribute to Biofilm Formation and Virulence in Trueperella pyogenes

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