Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging

Classic metabolomic methods have proven to be very useful to study functional biology and variation in the chemical composition of different tissues. However, they do not provide any information in terms of spatial localization within fine structures. Matrix-assisted laser desorption ionization mass...

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Autores principales:	Elvira Sgobba, Yohann Daguerre, Marco Giampà
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	MALDI MSI metabolomics imaging plants microbiology lipidomics Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/f0270a05e1be499999886e9966f8924d
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spelling	oai:doaj.org-article:f0270a05e1be499999886e9966f8924d2021-11-25T17:56:12ZUnravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging10.3390/ijms2222123931422-00671661-6596https://doaj.org/article/f0270a05e1be499999886e9966f8924d2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12393https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Classic metabolomic methods have proven to be very useful to study functional biology and variation in the chemical composition of different tissues. However, they do not provide any information in terms of spatial localization within fine structures. Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) does and reaches at best a spatial resolution of 0.25 μm depending on the laser setup, making it a very powerful tool to analyze the local complexity of biological samples at the cellular level. Here, we intend to give an overview of the diversity of the molecules and localizations analyzed using this method as well as to update on the latest adaptations made to circumvent the complexity of samples. MALDI MSI has been widely used in medical sciences and is now developing in research areas as diverse as entomology, microbiology, plant biology, and plant–microbe interactions, the rhizobia symbiosis being the most exhaustively described so far. Those are the fields of interest on which we will focus to demonstrate MALDI MSI strengths in characterizing the spatial distributions of metabolites, lipids, and peptides in relation to biological questions.Elvira SgobbaYohann DaguerreMarco GiampàMDPI AGarticleMALDI MSImetabolomicsimagingplantsmicrobiologylipidomicsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12393, p 12393 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MALDI MSI metabolomics imaging plants microbiology lipidomics Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	MALDI MSI metabolomics imaging plants microbiology lipidomics Biology (General) QH301-705.5 Chemistry QD1-999 Elvira Sgobba Yohann Daguerre Marco Giampà Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
description	Classic metabolomic methods have proven to be very useful to study functional biology and variation in the chemical composition of different tissues. However, they do not provide any information in terms of spatial localization within fine structures. Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) does and reaches at best a spatial resolution of 0.25 μm depending on the laser setup, making it a very powerful tool to analyze the local complexity of biological samples at the cellular level. Here, we intend to give an overview of the diversity of the molecules and localizations analyzed using this method as well as to update on the latest adaptations made to circumvent the complexity of samples. MALDI MSI has been widely used in medical sciences and is now developing in research areas as diverse as entomology, microbiology, plant biology, and plant–microbe interactions, the rhizobia symbiosis being the most exhaustively described so far. Those are the fields of interest on which we will focus to demonstrate MALDI MSI strengths in characterizing the spatial distributions of metabolites, lipids, and peptides in relation to biological questions.
format	article
author	Elvira Sgobba Yohann Daguerre Marco Giampà
author_facet	Elvira Sgobba Yohann Daguerre Marco Giampà
author_sort	Elvira Sgobba
title	Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
title_short	Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
title_full	Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
title_fullStr	Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
title_full_unstemmed	Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
title_sort	unravel the local complexity of biological environments by maldi mass spectrometry imaging
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f0270a05e1be499999886e9966f8924d
work_keys_str_mv	AT elvirasgobba unravelthelocalcomplexityofbiologicalenvironmentsbymaldimassspectrometryimaging AT yohanndaguerre unravelthelocalcomplexityofbiologicalenvironmentsbymaldimassspectrometryimaging AT marcogiampa unravelthelocalcomplexityofbiologicalenvironmentsbymaldimassspectrometryimaging
_version_	1718411838661591040

Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging

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