Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models

Objective To determine whether the addition of kerateine (reduced keratin) in rat tail collagen type I hydrogels increases thermal stability and changes material properties and supports cell growth for use in cellular hyperthermia studies for tumor treatment. Methods Collagen type I extracted from r...

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Auteurs principaux:	Kameel Zuniga, Manasa Gadde, Jacob Scheftel, Kris Senecal, Erik Cressman, Mark Van Dyke, Marissa Nichole Rylander
Format:	article
Langue:	EN
Publié:	Taylor & Francis Group 2021
Sujets:	hydrogel collagen kerateine hyperthermia biomimetic engineering Medical technology R855-855.5
Accès en ligne:	https://doaj.org/article/f0293c76a53a4384a597e826dab1be79
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spelling	oai:doaj.org-article:f0293c76a53a4384a597e826dab1be792021-11-11T14:23:40ZCollagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models0265-67361464-515710.1080/02656736.2021.1930202https://doaj.org/article/f0293c76a53a4384a597e826dab1be792021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/02656736.2021.1930202https://doaj.org/toc/0265-6736https://doaj.org/toc/1464-5157Objective To determine whether the addition of kerateine (reduced keratin) in rat tail collagen type I hydrogels increases thermal stability and changes material properties and supports cell growth for use in cellular hyperthermia studies for tumor treatment. Methods Collagen type I extracted from rat tail tendon was combined with kerateine extracted from human hair fibers. Thermal, mechanical, and biocompatibility properties and cell behavior was assessed and compared to 100% collagen type I hydrogels to demonstrate their utility as a tissue model for 3D in vitro testing. Results A combination (i.e., containing both collagen ‘C/KNT’) hydrogel was more thermally stable than pure collagen hydrogels and resisted thermal degradation when incubated at a hyperthermic temperature of 47°C for heating durations up to 60 min with a higher melting temperature measured by DSC. An increase in the storage modulus was only observed with an increased collagen concentration rather than an increased KTN concentration; however, a change in ECM structure was observed with greater fiber alignment and width with an increase in KTN concentration. The C/KTN hydrogels, specifically 50/50 C/KTN hydrogels, also supported the growth and of fibroblasts and MDA-MB-231 breast cancer cells similar to those seeded in 100% collagen hydrogels. Conclusion This multi-protein C/KTN hydrogel shows promise for future studies involving thermal stress studies without compromising the 3D ECM environment or cell growth.Kameel ZunigaManasa GaddeJacob ScheftelKris SenecalErik CressmanMark Van DykeMarissa Nichole RylanderTaylor & Francis Grouparticlehydrogelcollagenkerateinehyperthermiabiomimetic engineeringMedical technologyR855-855.5ENInternational Journal of Hyperthermia, Vol 38, Iss 1, Pp 830-845 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydrogel collagen kerateine hyperthermia biomimetic engineering Medical technology R855-855.5
spellingShingle	hydrogel collagen kerateine hyperthermia biomimetic engineering Medical technology R855-855.5 Kameel Zuniga Manasa Gadde Jacob Scheftel Kris Senecal Erik Cressman Mark Van Dyke Marissa Nichole Rylander Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models
description	Objective To determine whether the addition of kerateine (reduced keratin) in rat tail collagen type I hydrogels increases thermal stability and changes material properties and supports cell growth for use in cellular hyperthermia studies for tumor treatment. Methods Collagen type I extracted from rat tail tendon was combined with kerateine extracted from human hair fibers. Thermal, mechanical, and biocompatibility properties and cell behavior was assessed and compared to 100% collagen type I hydrogels to demonstrate their utility as a tissue model for 3D in vitro testing. Results A combination (i.e., containing both collagen ‘C/KNT’) hydrogel was more thermally stable than pure collagen hydrogels and resisted thermal degradation when incubated at a hyperthermic temperature of 47°C for heating durations up to 60 min with a higher melting temperature measured by DSC. An increase in the storage modulus was only observed with an increased collagen concentration rather than an increased KTN concentration; however, a change in ECM structure was observed with greater fiber alignment and width with an increase in KTN concentration. The C/KTN hydrogels, specifically 50/50 C/KTN hydrogels, also supported the growth and of fibroblasts and MDA-MB-231 breast cancer cells similar to those seeded in 100% collagen hydrogels. Conclusion This multi-protein C/KTN hydrogel shows promise for future studies involving thermal stress studies without compromising the 3D ECM environment or cell growth.
format	article
author	Kameel Zuniga Manasa Gadde Jacob Scheftel Kris Senecal Erik Cressman Mark Van Dyke Marissa Nichole Rylander
author_facet	Kameel Zuniga Manasa Gadde Jacob Scheftel Kris Senecal Erik Cressman Mark Van Dyke Marissa Nichole Rylander
author_sort	Kameel Zuniga
title	Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models
title_short	Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models
title_full	Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models
title_fullStr	Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models
title_full_unstemmed	Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models
title_sort	collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3d in vitro models
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/f0293c76a53a4384a597e826dab1be79
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Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models

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