Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae.

The continuous expansion of nosocomial infections around the globe has become a precarious situation. Key challenges include mounting dissemination of multiple resistances to antibiotics, the easy transmission and the growing mortality rates of hospital-acquired bacterial diseases. Thus, new ways to...

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Autores principales:	Sebastian Hoppe, Frank F Bier, Markus von Nickisch-Rosenegk
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f029dacb4baa41e993a8601bb8c16f0d
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spelling	oai:doaj.org-article:f029dacb4baa41e993a8601bb8c16f0d2021-11-25T05:55:50ZIdentification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae.1932-620310.1371/journal.pone.0110703https://doaj.org/article/f029dacb4baa41e993a8601bb8c16f0d2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0110703https://doaj.org/toc/1932-6203The continuous expansion of nosocomial infections around the globe has become a precarious situation. Key challenges include mounting dissemination of multiple resistances to antibiotics, the easy transmission and the growing mortality rates of hospital-acquired bacterial diseases. Thus, new ways to rapidly detect these infections are vital. Consequently, researchers around the globe pursue innovative approaches for point-of-care devices. In many cases the specific interaction of an antigen and a corresponding antibody is pivotal. However, the knowledge about suitable antigens is lacking. The aim of this study was to identify novel antigens as specific diagnostic markers. Additionally, these proteins might be aptly used for the generation of vaccines to improve current treatment options. Hence, a cDNA-based expression library was constructed and screened via microarrays to detect novel antigens of Klebsiella pneumoniae, a prominent agent of nosocomial infections well-known for its extensive antibiotics resistance, especially by extended-spectrum beta-lactamases (ESBL). After screening 1536 clones, 14 previously unknown immunogenic proteins were identified. Subsequently, each protein was expressed in full-length and its immunodominant character examined by ELISA and microarray analyses. Consequently, six proteins were selected for epitope mapping and three thereof possessed linear epitopes. After specificity analysis, homology survey and 3d structural modelling, one epitope sequence GAVVALSTTFA of KPN_00363, an ion channel protein, was identified harboring specificity for K. pneumoniae. The remaining epitopes showed ambiguous results regarding the specificity for K. pneumoniae. The approach adopted herein has been successfully utilized to discover novel antigens of Campylobacter jejuni and Salmonella enterica antigens before. Now, we have transferred this knowledge to the key nosocomial agent, K. pneumoniae. By identifying several novel antigens and their linear epitope sites, we have paved the way for crucial future research and applications including the design of point-of-care devices, vaccine development and serological screenings for a highly relevant nosocomial pathogen.Sebastian HoppeFrank F BierMarkus von Nickisch-RosenegkPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e110703 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sebastian Hoppe Frank F Bier Markus von Nickisch-Rosenegk Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae.
description	The continuous expansion of nosocomial infections around the globe has become a precarious situation. Key challenges include mounting dissemination of multiple resistances to antibiotics, the easy transmission and the growing mortality rates of hospital-acquired bacterial diseases. Thus, new ways to rapidly detect these infections are vital. Consequently, researchers around the globe pursue innovative approaches for point-of-care devices. In many cases the specific interaction of an antigen and a corresponding antibody is pivotal. However, the knowledge about suitable antigens is lacking. The aim of this study was to identify novel antigens as specific diagnostic markers. Additionally, these proteins might be aptly used for the generation of vaccines to improve current treatment options. Hence, a cDNA-based expression library was constructed and screened via microarrays to detect novel antigens of Klebsiella pneumoniae, a prominent agent of nosocomial infections well-known for its extensive antibiotics resistance, especially by extended-spectrum beta-lactamases (ESBL). After screening 1536 clones, 14 previously unknown immunogenic proteins were identified. Subsequently, each protein was expressed in full-length and its immunodominant character examined by ELISA and microarray analyses. Consequently, six proteins were selected for epitope mapping and three thereof possessed linear epitopes. After specificity analysis, homology survey and 3d structural modelling, one epitope sequence GAVVALSTTFA of KPN_00363, an ion channel protein, was identified harboring specificity for K. pneumoniae. The remaining epitopes showed ambiguous results regarding the specificity for K. pneumoniae. The approach adopted herein has been successfully utilized to discover novel antigens of Campylobacter jejuni and Salmonella enterica antigens before. Now, we have transferred this knowledge to the key nosocomial agent, K. pneumoniae. By identifying several novel antigens and their linear epitope sites, we have paved the way for crucial future research and applications including the design of point-of-care devices, vaccine development and serological screenings for a highly relevant nosocomial pathogen.
format	article
author	Sebastian Hoppe Frank F Bier Markus von Nickisch-Rosenegk
author_facet	Sebastian Hoppe Frank F Bier Markus von Nickisch-Rosenegk
author_sort	Sebastian Hoppe
title	Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae.
title_short	Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae.
title_full	Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae.
title_fullStr	Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae.
title_full_unstemmed	Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae.
title_sort	identification of antigenic proteins of the nosocomial pathogen klebsiella pneumoniae.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f029dacb4baa41e993a8601bb8c16f0d
work_keys_str_mv	AT sebastianhoppe identificationofantigenicproteinsofthenosocomialpathogenklebsiellapneumoniae AT frankfbier identificationofantigenicproteinsofthenosocomialpathogenklebsiellapneumoniae AT markusvonnickischrosenegk identificationofantigenicproteinsofthenosocomialpathogenklebsiellapneumoniae
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Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae.

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