Calycosin-7-O-β-Glucoside Isolated from <i>Astragalus membranaceus</i> Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells

Stem cells have received attention in various diseases, such as inflammatory, cancer, and bone diseases. Mesenchymal stem cells (MSCs) are multipotent stem cells that are critical for forming and repairing bone tissues. Herein, we isolated calycosin-7-O-β-glucoside (Caly) from the roots of <i>...

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Autores principales:	Kyung-Ran Park, Ji Eun Park, Bomi Kim, Il Keun Kwon, Jin Tae Hong, Hyung-Mun Yun
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	AKT ALP BMP2 Caly mineralization MSCs Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/f02ad3c32bc44af7b640930eb949da04
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spelling	oai:doaj.org-article:f02ad3c32bc44af7b640930eb949da042021-11-11T16:50:11ZCalycosin-7-O-β-Glucoside Isolated from <i>Astragalus membranaceus</i> Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells10.3390/ijms2221113621422-00671661-6596https://doaj.org/article/f02ad3c32bc44af7b640930eb949da042021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11362https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Stem cells have received attention in various diseases, such as inflammatory, cancer, and bone diseases. Mesenchymal stem cells (MSCs) are multipotent stem cells that are critical for forming and repairing bone tissues. Herein, we isolated calycosin-7-O-β-glucoside (Caly) from the roots of <i>Astragalus membranaceus</i>, which is one of the most famous medicinal herbs, and investigated the osteogenic activities of Caly in MSCs. Caly did not affect cytotoxicity against MSCs, whereas Caly enhanced cell migration during the osteogenesis of MSCs. Caly increased the expression and enzymatic activities of ALP and the formation of mineralized nodules during the osteogenesis of MSCs. The osteogenesis and bone-forming activities of Caly are mediated by bone morphogenetic protein 2 (BMP2), phospho-Smad1/5/8, Wnt3a, phospho-GSK3β, and phospho-AKT, inducing the expression of runt-related transcription factor 2 (RUNX2). In addition, Caly-mediated osteogenesis and RUNX2 expression were attenuated by noggin and wortmannin. Moreover, the effects were validated in pre-osteoblasts committed to the osteoblast lineages from MSCs. Overall, our results provide novel evidence that Caly stimulates osteoblast lineage commitment of MSCs by triggering RUNX2 expression, suggesting Caly as a potential anabolic drug to prevent bone diseases.Kyung-Ran ParkJi Eun ParkBomi KimIl Keun KwonJin Tae HongHyung-Mun YunMDPI AGarticleAKTALPBMP2CalymineralizationMSCsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11362, p 11362 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	AKT ALP BMP2 Caly mineralization MSCs Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	AKT ALP BMP2 Caly mineralization MSCs Biology (General) QH301-705.5 Chemistry QD1-999 Kyung-Ran Park Ji Eun Park Bomi Kim Il Keun Kwon Jin Tae Hong Hyung-Mun Yun Calycosin-7-O-β-Glucoside Isolated from <i>Astragalus membranaceus</i> Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells
description	Stem cells have received attention in various diseases, such as inflammatory, cancer, and bone diseases. Mesenchymal stem cells (MSCs) are multipotent stem cells that are critical for forming and repairing bone tissues. Herein, we isolated calycosin-7-O-β-glucoside (Caly) from the roots of <i>Astragalus membranaceus</i>, which is one of the most famous medicinal herbs, and investigated the osteogenic activities of Caly in MSCs. Caly did not affect cytotoxicity against MSCs, whereas Caly enhanced cell migration during the osteogenesis of MSCs. Caly increased the expression and enzymatic activities of ALP and the formation of mineralized nodules during the osteogenesis of MSCs. The osteogenesis and bone-forming activities of Caly are mediated by bone morphogenetic protein 2 (BMP2), phospho-Smad1/5/8, Wnt3a, phospho-GSK3β, and phospho-AKT, inducing the expression of runt-related transcription factor 2 (RUNX2). In addition, Caly-mediated osteogenesis and RUNX2 expression were attenuated by noggin and wortmannin. Moreover, the effects were validated in pre-osteoblasts committed to the osteoblast lineages from MSCs. Overall, our results provide novel evidence that Caly stimulates osteoblast lineage commitment of MSCs by triggering RUNX2 expression, suggesting Caly as a potential anabolic drug to prevent bone diseases.
format	article
author	Kyung-Ran Park Ji Eun Park Bomi Kim Il Keun Kwon Jin Tae Hong Hyung-Mun Yun
author_facet	Kyung-Ran Park Ji Eun Park Bomi Kim Il Keun Kwon Jin Tae Hong Hyung-Mun Yun
author_sort	Kyung-Ran Park
title	Calycosin-7-O-β-Glucoside Isolated from <i>Astragalus membranaceus</i> Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells
title_short	Calycosin-7-O-β-Glucoside Isolated from <i>Astragalus membranaceus</i> Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells
title_full	Calycosin-7-O-β-Glucoside Isolated from <i>Astragalus membranaceus</i> Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells
title_fullStr	Calycosin-7-O-β-Glucoside Isolated from <i>Astragalus membranaceus</i> Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells
title_full_unstemmed	Calycosin-7-O-β-Glucoside Isolated from <i>Astragalus membranaceus</i> Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells
title_sort	calycosin-7-o-β-glucoside isolated from <i>astragalus membranaceus</i> promotes osteogenesis and mineralization in human mesenchymal stem cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f02ad3c32bc44af7b640930eb949da04
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Calycosin-7-O-β-Glucoside Isolated from <i>Astragalus membranaceus</i> Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells

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