Molecular phylogeny and evolution of parabasalia with improved taxon sampling and new protein markers of actin and elongation factor-1α.

<h4>Background</h4>Inferring the evolutionary history of phylogenetically isolated, deep-branching groups of taxa-in particular determining the root-is often extraordinarily difficult because their close relatives are unavailable as suitable outgroups. One of these taxonomic groups is th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Satoko Noda, Cléa Mantini, Dionigia Meloni, Jun-Ichi Inoue, Osamu Kitade, Eric Viscogliosi, Moriya Ohkuma
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f036881efef14ce4b635885edafff1ed
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f036881efef14ce4b635885edafff1ed
record_format	dspace
spelling	oai:doaj.org-article:f036881efef14ce4b635885edafff1ed2021-11-18T07:30:41ZMolecular phylogeny and evolution of parabasalia with improved taxon sampling and new protein markers of actin and elongation factor-1α.1932-620310.1371/journal.pone.0029938https://doaj.org/article/f036881efef14ce4b635885edafff1ed2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22253832/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Inferring the evolutionary history of phylogenetically isolated, deep-branching groups of taxa-in particular determining the root-is often extraordinarily difficult because their close relatives are unavailable as suitable outgroups. One of these taxonomic groups is the phylum Parabasalia, which comprises morphologically diverse species of flagellated protists of ecological, medical, and evolutionary significance. Indeed, previous molecular phylogenetic analyses of members of this phylum have yielded conflicting and possibly erroneous inferences. Furthermore, many species of Parabasalia are symbionts in the gut of termites and cockroaches or parasites and therefore formidably difficult to cultivate, rendering available data insufficient. Increasing the numbers of examined taxa and informative characters (e.g., genes) is likely to produce more reliable inferences.<h4>Principal findings</h4>Actin and elongation factor-1α genes were identified newly from 22 species of termite-gut symbionts through careful manipulations and seven cultured species, which covered major lineages of Parabasalia. Their protein sequences were concatenated and analyzed with sequences of previously and newly identified glyceraldehyde-3-phosphate dehydrogenase and the small-subunit rRNA gene. This concatenated dataset provided more robust phylogenetic relationships among major groups of Parabasalia and a more plausible new root position than those previously reported.<h4>Conclusions/significance</h4>We conclude that increasing the number of sampled taxa as well as the addition of new sequences greatly improves the accuracy and robustness of the phylogenetic inference. A morphologically simple cell is likely the ancient form in Parabasalia as opposed to a cell with elaborate flagellar and cytoskeletal structures, which was defined as most basal in previous inferences. Nevertheless, the evolution of Parabasalia is complex owing to several independent multiplication and simplification events in these structures. Therefore, systematics based solely on morphology does not reflect the evolutionary history of parabasalids.Satoko NodaCléa MantiniDionigia MeloniJun-Ichi InoueOsamu KitadeEric ViscogliosiMoriya OhkumaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29938 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satoko Noda Cléa Mantini Dionigia Meloni Jun-Ichi Inoue Osamu Kitade Eric Viscogliosi Moriya Ohkuma Molecular phylogeny and evolution of parabasalia with improved taxon sampling and new protein markers of actin and elongation factor-1α.
description	<h4>Background</h4>Inferring the evolutionary history of phylogenetically isolated, deep-branching groups of taxa-in particular determining the root-is often extraordinarily difficult because their close relatives are unavailable as suitable outgroups. One of these taxonomic groups is the phylum Parabasalia, which comprises morphologically diverse species of flagellated protists of ecological, medical, and evolutionary significance. Indeed, previous molecular phylogenetic analyses of members of this phylum have yielded conflicting and possibly erroneous inferences. Furthermore, many species of Parabasalia are symbionts in the gut of termites and cockroaches or parasites and therefore formidably difficult to cultivate, rendering available data insufficient. Increasing the numbers of examined taxa and informative characters (e.g., genes) is likely to produce more reliable inferences.<h4>Principal findings</h4>Actin and elongation factor-1α genes were identified newly from 22 species of termite-gut symbionts through careful manipulations and seven cultured species, which covered major lineages of Parabasalia. Their protein sequences were concatenated and analyzed with sequences of previously and newly identified glyceraldehyde-3-phosphate dehydrogenase and the small-subunit rRNA gene. This concatenated dataset provided more robust phylogenetic relationships among major groups of Parabasalia and a more plausible new root position than those previously reported.<h4>Conclusions/significance</h4>We conclude that increasing the number of sampled taxa as well as the addition of new sequences greatly improves the accuracy and robustness of the phylogenetic inference. A morphologically simple cell is likely the ancient form in Parabasalia as opposed to a cell with elaborate flagellar and cytoskeletal structures, which was defined as most basal in previous inferences. Nevertheless, the evolution of Parabasalia is complex owing to several independent multiplication and simplification events in these structures. Therefore, systematics based solely on morphology does not reflect the evolutionary history of parabasalids.
format	article
author	Satoko Noda Cléa Mantini Dionigia Meloni Jun-Ichi Inoue Osamu Kitade Eric Viscogliosi Moriya Ohkuma
author_facet	Satoko Noda Cléa Mantini Dionigia Meloni Jun-Ichi Inoue Osamu Kitade Eric Viscogliosi Moriya Ohkuma
author_sort	Satoko Noda
title	Molecular phylogeny and evolution of parabasalia with improved taxon sampling and new protein markers of actin and elongation factor-1α.
title_short	Molecular phylogeny and evolution of parabasalia with improved taxon sampling and new protein markers of actin and elongation factor-1α.
title_full	Molecular phylogeny and evolution of parabasalia with improved taxon sampling and new protein markers of actin and elongation factor-1α.
title_fullStr	Molecular phylogeny and evolution of parabasalia with improved taxon sampling and new protein markers of actin and elongation factor-1α.
title_full_unstemmed	Molecular phylogeny and evolution of parabasalia with improved taxon sampling and new protein markers of actin and elongation factor-1α.
title_sort	molecular phylogeny and evolution of parabasalia with improved taxon sampling and new protein markers of actin and elongation factor-1α.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f036881efef14ce4b635885edafff1ed
work_keys_str_mv	AT satokonoda molecularphylogenyandevolutionofparabasaliawithimprovedtaxonsamplingandnewproteinmarkersofactinandelongationfactor1a AT cleamantini molecularphylogenyandevolutionofparabasaliawithimprovedtaxonsamplingandnewproteinmarkersofactinandelongationfactor1a AT dionigiameloni molecularphylogenyandevolutionofparabasaliawithimprovedtaxonsamplingandnewproteinmarkersofactinandelongationfactor1a AT junichiinoue molecularphylogenyandevolutionofparabasaliawithimprovedtaxonsamplingandnewproteinmarkersofactinandelongationfactor1a AT osamukitade molecularphylogenyandevolutionofparabasaliawithimprovedtaxonsamplingandnewproteinmarkersofactinandelongationfactor1a AT ericviscogliosi molecularphylogenyandevolutionofparabasaliawithimprovedtaxonsamplingandnewproteinmarkersofactinandelongationfactor1a AT moriyaohkuma molecularphylogenyandevolutionofparabasaliawithimprovedtaxonsamplingandnewproteinmarkersofactinandelongationfactor1a
_version_	1718423385769246720

Molecular phylogeny and evolution of parabasalia with improved taxon sampling and new protein markers of actin and elongation factor-1α.

Ejemplares similares