Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt

Abstract The newt, a group of urodele amphibians, has outstanding ability to repeatedly regenerate various body parts, even in the terrestrial life-stage. In this animal, when the limb is amputated, a cell mass named the blastema appears on the stump and eventually gives rise to a new functional lim...

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Autores principales:	Roman M. Casco-Robles, Akihiko Watanabe, Ko Eto, Kazuhito Takeshima, Shuichi Obata, Tsutomu Kinoshita, Takashi Ariizumi, Kei Nakatani, Tomoaki Nakada, Panagiotis A. Tsonis, Martin M. Casco-Robles, Keisuke Sakurai, Kensuke Yahata, Fumiaki Maruo, Fubito Toyama, Chikafumi Chiba
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f040dc66b27041e689abf7b2d22d882d
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spelling	oai:doaj.org-article:f040dc66b27041e689abf7b2d22d882d2021-12-02T15:07:58ZNovel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt10.1038/s41598-018-25867-x2045-2322https://doaj.org/article/f040dc66b27041e689abf7b2d22d882d2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25867-xhttps://doaj.org/toc/2045-2322Abstract The newt, a group of urodele amphibians, has outstanding ability to repeatedly regenerate various body parts, even in the terrestrial life-stage. In this animal, when the limb is amputated, a cell mass named the blastema appears on the stump and eventually gives rise to a new functional limb. Erythrocytes (red blood cells) in most non-mammalian vertebrates, including the newt, preserve their nucleus throughout their life-span, although physiological roles of such nucleated erythrocytes, other than oxygen delivery, are not known. Here we report novel behavior of erythrocytes in the newt. We identified an orphan gene Newtic1, whose transcripts significantly increased in the blastema. Newtic1 was expressed in a subset of erythrocytes that formed a novel clump (EryC). EryC formed a complex with monocytes and was circulating throughout the body. When the limb was amputated, EryCs were newly generated in the stump and accumulated into a distal portion of the growing blastema. Our data suggested that the newt erythrocytes carried multiple secretory molecules including growth factors and matrix metalloproteases, and were capable of delivering these molecules into the blastema as a form of EryCs. This study provides insight into regulations and roles of nucleated erythrocytes, that are independent of oxygen delivery.Roman M. Casco-RoblesAkihiko WatanabeKo EtoKazuhito TakeshimaShuichi ObataTsutomu KinoshitaTakashi AriizumiKei NakataniTomoaki NakadaPanagiotis A. TsonisMartin M. Casco-RoblesKeisuke SakuraiKensuke YahataFumiaki MaruoFubito ToyamaChikafumi ChibaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Roman M. Casco-Robles Akihiko Watanabe Ko Eto Kazuhito Takeshima Shuichi Obata Tsutomu Kinoshita Takashi Ariizumi Kei Nakatani Tomoaki Nakada Panagiotis A. Tsonis Martin M. Casco-Robles Keisuke Sakurai Kensuke Yahata Fumiaki Maruo Fubito Toyama Chikafumi Chiba Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt
description	Abstract The newt, a group of urodele amphibians, has outstanding ability to repeatedly regenerate various body parts, even in the terrestrial life-stage. In this animal, when the limb is amputated, a cell mass named the blastema appears on the stump and eventually gives rise to a new functional limb. Erythrocytes (red blood cells) in most non-mammalian vertebrates, including the newt, preserve their nucleus throughout their life-span, although physiological roles of such nucleated erythrocytes, other than oxygen delivery, are not known. Here we report novel behavior of erythrocytes in the newt. We identified an orphan gene Newtic1, whose transcripts significantly increased in the blastema. Newtic1 was expressed in a subset of erythrocytes that formed a novel clump (EryC). EryC formed a complex with monocytes and was circulating throughout the body. When the limb was amputated, EryCs were newly generated in the stump and accumulated into a distal portion of the growing blastema. Our data suggested that the newt erythrocytes carried multiple secretory molecules including growth factors and matrix metalloproteases, and were capable of delivering these molecules into the blastema as a form of EryCs. This study provides insight into regulations and roles of nucleated erythrocytes, that are independent of oxygen delivery.
format	article
author	Roman M. Casco-Robles Akihiko Watanabe Ko Eto Kazuhito Takeshima Shuichi Obata Tsutomu Kinoshita Takashi Ariizumi Kei Nakatani Tomoaki Nakada Panagiotis A. Tsonis Martin M. Casco-Robles Keisuke Sakurai Kensuke Yahata Fumiaki Maruo Fubito Toyama Chikafumi Chiba
author_facet	Roman M. Casco-Robles Akihiko Watanabe Ko Eto Kazuhito Takeshima Shuichi Obata Tsutomu Kinoshita Takashi Ariizumi Kei Nakatani Tomoaki Nakada Panagiotis A. Tsonis Martin M. Casco-Robles Keisuke Sakurai Kensuke Yahata Fumiaki Maruo Fubito Toyama Chikafumi Chiba
author_sort	Roman M. Casco-Robles
title	Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt
title_short	Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt
title_full	Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt
title_fullStr	Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt
title_full_unstemmed	Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt
title_sort	novel erythrocyte clumps revealed by an orphan gene newtic1 in circulating blood and regenerating limbs of the adult newt
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f040dc66b27041e689abf7b2d22d882d
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Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt

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