Identification of oxytocin-related lncRNAs and assessment of their expression in breast cancer

Abstract Oxytocin is a neuropeptide released by the central nervous system. A number of studies have demonstrated the role of this neuropeptide in the pathogenesis of breast cancer. In the present project, we have identified mRNA coding genes and long non-coding RNAs (lncRNAs) that are associated wi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sepehr Behtaji, Soudeh Ghafouri-Fard, Arezou Sayad, Ali Sattari, Mathieu Rederstorff, Mohammad Taheri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f051ac4e5c99452eada66516c118fcda
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f051ac4e5c99452eada66516c118fcda
record_format	dspace
spelling	oai:doaj.org-article:f051ac4e5c99452eada66516c118fcda2021-12-02T16:31:07ZIdentification of oxytocin-related lncRNAs and assessment of their expression in breast cancer10.1038/s41598-021-86097-22045-2322https://doaj.org/article/f051ac4e5c99452eada66516c118fcda2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86097-2https://doaj.org/toc/2045-2322Abstract Oxytocin is a neuropeptide released by the central nervous system. A number of studies have demonstrated the role of this neuropeptide in the pathogenesis of breast cancer. In the present project, we have identified mRNA coding genes and long non-coding RNAs (lncRNAs) that are associated with this pathway through an in-silico strategy, and measured their expression in a cohort of Iranian females affected with this type of malignancy. Expression levels of OXTR, FOS, ITPR1, RCAN1, CAMK2D, CACNA2D and lnc_ZFP161 were significantly down-regulated in breast cancer tissues compared with nearby non-cancerous tissues. On the other hand, expression of lnc_MTX2 was higher in breast cancer tissues compared with controls. Expression of lnc_TNS1 and lnc_FOXF1 were not different between these two kinds of samples. Expression of CACNA2D was associated with mitotic rate and PR status (P values = 3.02E−02 and 2.53E−02, respectively). Expression of other oxytocin-related genes was not associated with clinicopathological parameters. FOS and ITPR1 had the highest AUC value among the oxytocin-related genes. Combination of expression profiles of all oxytocin-related genes increased the AUC value to 0.75. However, the combinatorial sensitivity and specificity values were lower than some individual genes. In the breast cancer tissues, the most robust correlations have been detected between lnc_ZFP161/ lnc_FOXF1, CAMK2D/ lnc_ZFP161 and CAMK2D / lnc_FOXF1 (r = 0.86, 0.71 and 0.64 respectively). In the non-cancerous tissues, the strongest correlation was detected between lnc_FOXF1/lnc_MTX2 and lnc_ZFP161/CAMK2D respectively (r = 0.78 and 0.65). Taken together, oxytocin-associated genes have been dysregulated in breast cancer tissues. Moreover, the correlation ratio between these genes is connected with the existence of cancer.Sepehr BehtajiSoudeh Ghafouri-FardArezou SayadAli SattariMathieu RederstorffMohammad TaheriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sepehr Behtaji Soudeh Ghafouri-Fard Arezou Sayad Ali Sattari Mathieu Rederstorff Mohammad Taheri Identification of oxytocin-related lncRNAs and assessment of their expression in breast cancer
description	Abstract Oxytocin is a neuropeptide released by the central nervous system. A number of studies have demonstrated the role of this neuropeptide in the pathogenesis of breast cancer. In the present project, we have identified mRNA coding genes and long non-coding RNAs (lncRNAs) that are associated with this pathway through an in-silico strategy, and measured their expression in a cohort of Iranian females affected with this type of malignancy. Expression levels of OXTR, FOS, ITPR1, RCAN1, CAMK2D, CACNA2D and lnc_ZFP161 were significantly down-regulated in breast cancer tissues compared with nearby non-cancerous tissues. On the other hand, expression of lnc_MTX2 was higher in breast cancer tissues compared with controls. Expression of lnc_TNS1 and lnc_FOXF1 were not different between these two kinds of samples. Expression of CACNA2D was associated with mitotic rate and PR status (P values = 3.02E−02 and 2.53E−02, respectively). Expression of other oxytocin-related genes was not associated with clinicopathological parameters. FOS and ITPR1 had the highest AUC value among the oxytocin-related genes. Combination of expression profiles of all oxytocin-related genes increased the AUC value to 0.75. However, the combinatorial sensitivity and specificity values were lower than some individual genes. In the breast cancer tissues, the most robust correlations have been detected between lnc_ZFP161/ lnc_FOXF1, CAMK2D/ lnc_ZFP161 and CAMK2D / lnc_FOXF1 (r = 0.86, 0.71 and 0.64 respectively). In the non-cancerous tissues, the strongest correlation was detected between lnc_FOXF1/lnc_MTX2 and lnc_ZFP161/CAMK2D respectively (r = 0.78 and 0.65). Taken together, oxytocin-associated genes have been dysregulated in breast cancer tissues. Moreover, the correlation ratio between these genes is connected with the existence of cancer.
format	article
author	Sepehr Behtaji Soudeh Ghafouri-Fard Arezou Sayad Ali Sattari Mathieu Rederstorff Mohammad Taheri
author_facet	Sepehr Behtaji Soudeh Ghafouri-Fard Arezou Sayad Ali Sattari Mathieu Rederstorff Mohammad Taheri
author_sort	Sepehr Behtaji
title	Identification of oxytocin-related lncRNAs and assessment of their expression in breast cancer
title_short	Identification of oxytocin-related lncRNAs and assessment of their expression in breast cancer
title_full	Identification of oxytocin-related lncRNAs and assessment of their expression in breast cancer
title_fullStr	Identification of oxytocin-related lncRNAs and assessment of their expression in breast cancer
title_full_unstemmed	Identification of oxytocin-related lncRNAs and assessment of their expression in breast cancer
title_sort	identification of oxytocin-related lncrnas and assessment of their expression in breast cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f051ac4e5c99452eada66516c118fcda
work_keys_str_mv	AT sepehrbehtaji identificationofoxytocinrelatedlncrnasandassessmentoftheirexpressioninbreastcancer AT soudehghafourifard identificationofoxytocinrelatedlncrnasandassessmentoftheirexpressioninbreastcancer AT arezousayad identificationofoxytocinrelatedlncrnasandassessmentoftheirexpressioninbreastcancer AT alisattari identificationofoxytocinrelatedlncrnasandassessmentoftheirexpressioninbreastcancer AT mathieurederstorff identificationofoxytocinrelatedlncrnasandassessmentoftheirexpressioninbreastcancer AT mohammadtaheri identificationofoxytocinrelatedlncrnasandassessmentoftheirexpressioninbreastcancer
_version_	1718383908047814656

Identification of oxytocin-related lncRNAs and assessment of their expression in breast cancer

Ejemplares similares