Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen.

Most current clinical vaccines work primarily by inducing the production of neutralizing antibodies against pathogens. Vaccine adjuvants that efficiently induce T cell responses to protein antigens need to be developed. In this study, we developed a new combination adjuvant consisting of 1,2-dioleoy...

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Autores principales:	Yasunari Haseda, Lisa Munakata, Chiyo Kimura, Yumi Kinugasa-Katayama, Yasuko Mori, Ryo Suzuki, Taiki Aoshi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f056d23d425b42a28ef189306b7b38ec
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spelling	oai:doaj.org-article:f056d23d425b42a28ef189306b7b38ec2021-12-02T20:18:53ZDevelopment of combination adjuvant for efficient T cell and antibody response induction against protein antigen.1932-620310.1371/journal.pone.0254628https://doaj.org/article/f056d23d425b42a28ef189306b7b38ec2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254628https://doaj.org/toc/1932-6203Most current clinical vaccines work primarily by inducing the production of neutralizing antibodies against pathogens. Vaccine adjuvants that efficiently induce T cell responses to protein antigens need to be developed. In this study, we developed a new combination adjuvant consisting of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), D35, and an aluminum salt. Among the various combinations tested, the DOTAP/D35/aluminum salt adjuvant induced strong T cell and antibody responses against the model protein antigen with a single immunization. Adjuvant component and model antigen interaction studies in vitro also revealed that the strong mutual interactions among protein antigens and other components were one of the important factors for this efficient immune induction by the novel combination adjuvant. In addition, in vivo imaging of the antigen distribution suggested that the DOTAP component in the combination adjuvant formulation elicited transient antigen accumulation at the draining lymph nodes, possibly by antigen uptake DC migration. These results indicate the potential of the new combination adjuvant as a promising vaccine adjuvant candidate to treat infectious diseases and cancers.Yasunari HasedaLisa MunakataChiyo KimuraYumi Kinugasa-KatayamaYasuko MoriRyo SuzukiTaiki AoshiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0254628 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yasunari Haseda Lisa Munakata Chiyo Kimura Yumi Kinugasa-Katayama Yasuko Mori Ryo Suzuki Taiki Aoshi Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen.
description	Most current clinical vaccines work primarily by inducing the production of neutralizing antibodies against pathogens. Vaccine adjuvants that efficiently induce T cell responses to protein antigens need to be developed. In this study, we developed a new combination adjuvant consisting of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), D35, and an aluminum salt. Among the various combinations tested, the DOTAP/D35/aluminum salt adjuvant induced strong T cell and antibody responses against the model protein antigen with a single immunization. Adjuvant component and model antigen interaction studies in vitro also revealed that the strong mutual interactions among protein antigens and other components were one of the important factors for this efficient immune induction by the novel combination adjuvant. In addition, in vivo imaging of the antigen distribution suggested that the DOTAP component in the combination adjuvant formulation elicited transient antigen accumulation at the draining lymph nodes, possibly by antigen uptake DC migration. These results indicate the potential of the new combination adjuvant as a promising vaccine adjuvant candidate to treat infectious diseases and cancers.
format	article
author	Yasunari Haseda Lisa Munakata Chiyo Kimura Yumi Kinugasa-Katayama Yasuko Mori Ryo Suzuki Taiki Aoshi
author_facet	Yasunari Haseda Lisa Munakata Chiyo Kimura Yumi Kinugasa-Katayama Yasuko Mori Ryo Suzuki Taiki Aoshi
author_sort	Yasunari Haseda
title	Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen.
title_short	Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen.
title_full	Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen.
title_fullStr	Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen.
title_full_unstemmed	Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen.
title_sort	development of combination adjuvant for efficient t cell and antibody response induction against protein antigen.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f056d23d425b42a28ef189306b7b38ec
work_keys_str_mv	AT yasunarihaseda developmentofcombinationadjuvantforefficienttcellandantibodyresponseinductionagainstproteinantigen AT lisamunakata developmentofcombinationadjuvantforefficienttcellandantibodyresponseinductionagainstproteinantigen AT chiyokimura developmentofcombinationadjuvantforefficienttcellandantibodyresponseinductionagainstproteinantigen AT yumikinugasakatayama developmentofcombinationadjuvantforefficienttcellandantibodyresponseinductionagainstproteinantigen AT yasukomori developmentofcombinationadjuvantforefficienttcellandantibodyresponseinductionagainstproteinantigen AT ryosuzuki developmentofcombinationadjuvantforefficienttcellandantibodyresponseinductionagainstproteinantigen AT taikiaoshi developmentofcombinationadjuvantforefficienttcellandantibodyresponseinductionagainstproteinantigen
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Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen.

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