USING REAL-TIME PCR FOR URGENT DETECTION AND PROPHYLAXIS OF TICK-BORNE INFECTIONS

The urgent detection of tick-borne pathogens in attached ticks or in human blood followed by the prophylaxis of infection with specific immunoglobulin and/or antibiotics is one of the most reliable measures to prevent human disease. The aim of this work was to evaluate the usability of real-time PCR...

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Autores principales:	A. V. Lyapunov, M. A. Khasnatinov, E. L. Manzarova, N. A. Bolotova, G. A. Danchinova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2016
Materias:	ixodid ticks tick-borne encephalitis lyme disease human monocytic ehrlichiosis human granulocytic anaplasmosis diagnostics preventive treatment pcr Science Q
Acceso en línea:	https://doaj.org/article/f05ca685d1f14eb492b9f56355579872
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spelling	oai:doaj.org-article:f05ca685d1f14eb492b9f563555798722021-11-23T06:14:36ZUSING REAL-TIME PCR FOR URGENT DETECTION AND PROPHYLAXIS OF TICK-BORNE INFECTIONS2541-94202587-959610.12737/23810https://doaj.org/article/f05ca685d1f14eb492b9f563555798722016-11-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/341https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596The urgent detection of tick-borne pathogens in attached ticks or in human blood followed by the prophylaxis of infection with specific immunoglobulin and/or antibiotics is one of the most reliable measures to prevent human disease. The aim of this work was to evaluate the usability of real-time PCR (RT-PCR) assay in early detection of tick-borne infections. Using commercial RT-PCR assay (Amplisens, Russia) we studied 361 Ixodes persulcatus ticks, 33 Dermacentor sp. ticks and 448 samples of human blood delivered by people attacked by ticks in Irkutsk region during 2013-2014for presence of DNA/RNA of tick-borne encephalitis virus (TBEV), Borrelia burgdorferi sensu lato (B.b.s.l.), Anaplsma phagocytophilum and Ehrlichia chaffeensis/E. muris. The ELISA assay and direct microscopy were used to validate the detection of TBEV and B.b.s.l. respectively. The highest prevalence of tick-borne infections was observed for I. persulcatus ticks - 6,9; 23,8; 6,9; and 11,9 % for TBEV, B.b.s.l., A. phagocytophillum and E. chaffeensis/E. muris respectively. The Dermacentor sp. ticks were less frequently infected with abovementioned pathogens - 3,0; 6,1; 3,0 and 9,1 % respectively. The prevalence of infection in blood samples was lowest and comprised 2,5; 4,2; 2,2 and 4,5 % respectively. Mixed infections were documented in 6,5 % of ticks and 0,7 % of human blood samples. The testing of blood samples in ELISA and testing of ticks in ELISA and direct microscopy corresponded to the results, obtained by RT-PCR. Thus, RT-PCR is valuable and reliable approach for urgent detection and prophylaxis of tick-borne infections.A. V. LyapunovM. A. KhasnatinovE. L. ManzarovaN. A. BolotovaG. A. DanchinovaScientific Сentre for Family Health and Human Reproduction Problemsarticleixodid tickstick-borne encephalitislyme diseasehuman monocytic ehrlichiosishuman granulocytic anaplasmosisdiagnosticspreventive treatmentpcrScienceQRUActa Biomedica Scientifica, Vol 1, Iss 6, Pp 161-166 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	ixodid ticks tick-borne encephalitis lyme disease human monocytic ehrlichiosis human granulocytic anaplasmosis diagnostics preventive treatment pcr Science Q
spellingShingle	ixodid ticks tick-borne encephalitis lyme disease human monocytic ehrlichiosis human granulocytic anaplasmosis diagnostics preventive treatment pcr Science Q A. V. Lyapunov M. A. Khasnatinov E. L. Manzarova N. A. Bolotova G. A. Danchinova USING REAL-TIME PCR FOR URGENT DETECTION AND PROPHYLAXIS OF TICK-BORNE INFECTIONS
description	The urgent detection of tick-borne pathogens in attached ticks or in human blood followed by the prophylaxis of infection with specific immunoglobulin and/or antibiotics is one of the most reliable measures to prevent human disease. The aim of this work was to evaluate the usability of real-time PCR (RT-PCR) assay in early detection of tick-borne infections. Using commercial RT-PCR assay (Amplisens, Russia) we studied 361 Ixodes persulcatus ticks, 33 Dermacentor sp. ticks and 448 samples of human blood delivered by people attacked by ticks in Irkutsk region during 2013-2014for presence of DNA/RNA of tick-borne encephalitis virus (TBEV), Borrelia burgdorferi sensu lato (B.b.s.l.), Anaplsma phagocytophilum and Ehrlichia chaffeensis/E. muris. The ELISA assay and direct microscopy were used to validate the detection of TBEV and B.b.s.l. respectively. The highest prevalence of tick-borne infections was observed for I. persulcatus ticks - 6,9; 23,8; 6,9; and 11,9 % for TBEV, B.b.s.l., A. phagocytophillum and E. chaffeensis/E. muris respectively. The Dermacentor sp. ticks were less frequently infected with abovementioned pathogens - 3,0; 6,1; 3,0 and 9,1 % respectively. The prevalence of infection in blood samples was lowest and comprised 2,5; 4,2; 2,2 and 4,5 % respectively. Mixed infections were documented in 6,5 % of ticks and 0,7 % of human blood samples. The testing of blood samples in ELISA and testing of ticks in ELISA and direct microscopy corresponded to the results, obtained by RT-PCR. Thus, RT-PCR is valuable and reliable approach for urgent detection and prophylaxis of tick-borne infections.
format	article
author	A. V. Lyapunov M. A. Khasnatinov E. L. Manzarova N. A. Bolotova G. A. Danchinova
author_facet	A. V. Lyapunov M. A. Khasnatinov E. L. Manzarova N. A. Bolotova G. A. Danchinova
author_sort	A. V. Lyapunov
title	USING REAL-TIME PCR FOR URGENT DETECTION AND PROPHYLAXIS OF TICK-BORNE INFECTIONS
title_short	USING REAL-TIME PCR FOR URGENT DETECTION AND PROPHYLAXIS OF TICK-BORNE INFECTIONS
title_full	USING REAL-TIME PCR FOR URGENT DETECTION AND PROPHYLAXIS OF TICK-BORNE INFECTIONS
title_fullStr	USING REAL-TIME PCR FOR URGENT DETECTION AND PROPHYLAXIS OF TICK-BORNE INFECTIONS
title_full_unstemmed	USING REAL-TIME PCR FOR URGENT DETECTION AND PROPHYLAXIS OF TICK-BORNE INFECTIONS
title_sort	using real-time pcr for urgent detection and prophylaxis of tick-borne infections
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2016
url	https://doaj.org/article/f05ca685d1f14eb492b9f56355579872
work_keys_str_mv	AT avlyapunov usingrealtimepcrforurgentdetectionandprophylaxisoftickborneinfections AT makhasnatinov usingrealtimepcrforurgentdetectionandprophylaxisoftickborneinfections AT elmanzarova usingrealtimepcrforurgentdetectionandprophylaxisoftickborneinfections AT nabolotova usingrealtimepcrforurgentdetectionandprophylaxisoftickborneinfections AT gadanchinova usingrealtimepcrforurgentdetectionandprophylaxisoftickborneinfections
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USING REAL-TIME PCR FOR URGENT DETECTION AND PROPHYLAXIS OF TICK-BORNE INFECTIONS

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