Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids

Abstract Paper-based biosensors based on lateral flow immunoassay (LFI) are promising candidates for POC diagnosis because of their ease of use and rapid target detection. However, the low sensitivity of LFI limits its application, and signal amplification has been used in numerous studies to increa...

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Autores principales:	Hyun-Kyung Oh, Kihyeun Kim, Jinhee Park, Hyungjun Jang, Min-Gon Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f06157055a564f7187a1919902eb4eee
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spelling	oai:doaj.org-article:f06157055a564f7187a1919902eb4eee2021-11-21T12:18:48ZAdvanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids10.1038/s41598-021-02084-72045-2322https://doaj.org/article/f06157055a564f7187a1919902eb4eee2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02084-7https://doaj.org/toc/2045-2322Abstract Paper-based biosensors based on lateral flow immunoassay (LFI) are promising candidates for POC diagnosis because of their ease of use and rapid target detection. However, the low sensitivity of LFI limits its application, and signal amplification has been used in numerous studies to increase its sensitivity. We developed an advanced trap LFI (α-trapLFI), a simple-to-use sensor, with an additional step for signal amplification. Here, signal amplification is automatically implemented following delayed release of enhancement solution induced by water-soluble polyvinyl alcohol tape. As the polyvinyl alcohol tape is exposed to water, its polymer structure is perturbed (within 5 min), allowing ions to pass through. This new sensor was designed to have a short time delay between the flow of solutions used for the immunoassay and signal amplification. The α-trapLFI was subsequently used to detect cortisol with high sensitivity (9.1 pg∙mL−1) over a broad detection range (0.01–1000 ng∙mL−1) in bodily fluids. Furthermore, an excellent correlation was obtained by analyzing 20 human real saliva samples using this sensor and a conventional ELISA (R 2 = 0.90). The new sensor will be helpful in detecting various small molecules for simple, rapid, and portable POC diagnosis of stress disorders.Hyun-Kyung OhKihyeun KimJinhee ParkHyungjun JangMin-Gon KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyun-Kyung Oh Kihyeun Kim Jinhee Park Hyungjun Jang Min-Gon Kim Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids
description	Abstract Paper-based biosensors based on lateral flow immunoassay (LFI) are promising candidates for POC diagnosis because of their ease of use and rapid target detection. However, the low sensitivity of LFI limits its application, and signal amplification has been used in numerous studies to increase its sensitivity. We developed an advanced trap LFI (α-trapLFI), a simple-to-use sensor, with an additional step for signal amplification. Here, signal amplification is automatically implemented following delayed release of enhancement solution induced by water-soluble polyvinyl alcohol tape. As the polyvinyl alcohol tape is exposed to water, its polymer structure is perturbed (within 5 min), allowing ions to pass through. This new sensor was designed to have a short time delay between the flow of solutions used for the immunoassay and signal amplification. The α-trapLFI was subsequently used to detect cortisol with high sensitivity (9.1 pg∙mL−1) over a broad detection range (0.01–1000 ng∙mL−1) in bodily fluids. Furthermore, an excellent correlation was obtained by analyzing 20 human real saliva samples using this sensor and a conventional ELISA (R 2 = 0.90). The new sensor will be helpful in detecting various small molecules for simple, rapid, and portable POC diagnosis of stress disorders.
format	article
author	Hyun-Kyung Oh Kihyeun Kim Jinhee Park Hyungjun Jang Min-Gon Kim
author_facet	Hyun-Kyung Oh Kihyeun Kim Jinhee Park Hyungjun Jang Min-Gon Kim
author_sort	Hyun-Kyung Oh
title	Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids
title_short	Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids
title_full	Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids
title_fullStr	Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids
title_full_unstemmed	Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids
title_sort	advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f06157055a564f7187a1919902eb4eee
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Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids

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