Characterization of an anterior segment organ culture model for open globe injuries

Abstract Open-globe injuries have poor visual outcomes and have increased in frequency. The current standard of care is inadequate, and a therapeutic is needed to stabilize the injury until an ophthalmic specialist is reached. Unfortunately, current models or test platforms for open-globe injuries a...

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Autores principales:	Eric J. Snider, Emily N. Boice, Brandon Gross, Jacinque J. Butler, David O. Zamora
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f0621768822941e1a6c2cd91ccd4eba7
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spelling	oai:doaj.org-article:f0621768822941e1a6c2cd91ccd4eba72021-12-02T13:39:34ZCharacterization of an anterior segment organ culture model for open globe injuries10.1038/s41598-021-87910-82045-2322https://doaj.org/article/f0621768822941e1a6c2cd91ccd4eba72021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87910-8https://doaj.org/toc/2045-2322Abstract Open-globe injuries have poor visual outcomes and have increased in frequency. The current standard of care is inadequate, and a therapeutic is needed to stabilize the injury until an ophthalmic specialist is reached. Unfortunately, current models or test platforms for open-globe injuries are insufficient. Here, we develop and characterize an open-globe injury model using an anterior segment organ-culture platform that allows therapeutic assessment for up to 72 h post-injury. Anterior segments maintained in organ culture were kept at physiological intraocular pressure throughout, and puncture injuries were created using a novel pneumatic-powered system. This system can create high-speed, military-relevant injuries up to 4.5 mm in diameter through the cornea. From intraocular pressure readings, we confirmed a loss of pressure across the 72 h after open-globe injury. Proof-of-concept studies with a Dermabond tissue adhesive were performed to show how this model system could track therapeutic performance for 72 h. Overall, the organ-culture platform was found to be a suitable next step towards modeling open-globe injuries and assessing wound closure over the critical 72 h post-injury. With improved models such as this, novel biomaterial therapeutics development can be accelerated, improving care, and, thus, improving the prognosis for the patients.Eric J. SniderEmily N. BoiceBrandon GrossJacinque J. ButlerDavid O. ZamoraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eric J. Snider Emily N. Boice Brandon Gross Jacinque J. Butler David O. Zamora Characterization of an anterior segment organ culture model for open globe injuries
description	Abstract Open-globe injuries have poor visual outcomes and have increased in frequency. The current standard of care is inadequate, and a therapeutic is needed to stabilize the injury until an ophthalmic specialist is reached. Unfortunately, current models or test platforms for open-globe injuries are insufficient. Here, we develop and characterize an open-globe injury model using an anterior segment organ-culture platform that allows therapeutic assessment for up to 72 h post-injury. Anterior segments maintained in organ culture were kept at physiological intraocular pressure throughout, and puncture injuries were created using a novel pneumatic-powered system. This system can create high-speed, military-relevant injuries up to 4.5 mm in diameter through the cornea. From intraocular pressure readings, we confirmed a loss of pressure across the 72 h after open-globe injury. Proof-of-concept studies with a Dermabond tissue adhesive were performed to show how this model system could track therapeutic performance for 72 h. Overall, the organ-culture platform was found to be a suitable next step towards modeling open-globe injuries and assessing wound closure over the critical 72 h post-injury. With improved models such as this, novel biomaterial therapeutics development can be accelerated, improving care, and, thus, improving the prognosis for the patients.
format	article
author	Eric J. Snider Emily N. Boice Brandon Gross Jacinque J. Butler David O. Zamora
author_facet	Eric J. Snider Emily N. Boice Brandon Gross Jacinque J. Butler David O. Zamora
author_sort	Eric J. Snider
title	Characterization of an anterior segment organ culture model for open globe injuries
title_short	Characterization of an anterior segment organ culture model for open globe injuries
title_full	Characterization of an anterior segment organ culture model for open globe injuries
title_fullStr	Characterization of an anterior segment organ culture model for open globe injuries
title_full_unstemmed	Characterization of an anterior segment organ culture model for open globe injuries
title_sort	characterization of an anterior segment organ culture model for open globe injuries
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f0621768822941e1a6c2cd91ccd4eba7
work_keys_str_mv	AT ericjsnider characterizationofananteriorsegmentorganculturemodelforopenglobeinjuries AT emilynboice characterizationofananteriorsegmentorganculturemodelforopenglobeinjuries AT brandongross characterizationofananteriorsegmentorganculturemodelforopenglobeinjuries AT jacinquejbutler characterizationofananteriorsegmentorganculturemodelforopenglobeinjuries AT davidozamora characterizationofananteriorsegmentorganculturemodelforopenglobeinjuries
_version_	1718392652187041792

Characterization of an anterior segment organ culture model for open globe injuries

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