Nitrogen isotopes provide clues to amino acid metabolism in human colorectal cancer cells

Abstract Glutamic acid and alanine make up more than 60 per cent of the total amino acids in the human body. Glutamine is a significant source of energy for cells and also a prime donor of nitrogen in the biosynthesis of many amino acids. Several studies have advocated the role of glutamic acid in c...

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Autores principales:	R. V. Krishnamurthy, Yogesh R. Suryawanshi, Karim Essani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f062f5b9c7f74cbbb9dccf10520e6f50
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spelling	oai:doaj.org-article:f062f5b9c7f74cbbb9dccf10520e6f502021-12-02T15:06:16ZNitrogen isotopes provide clues to amino acid metabolism in human colorectal cancer cells10.1038/s41598-017-02793-y2045-2322https://doaj.org/article/f062f5b9c7f74cbbb9dccf10520e6f502017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02793-yhttps://doaj.org/toc/2045-2322Abstract Glutamic acid and alanine make up more than 60 per cent of the total amino acids in the human body. Glutamine is a significant source of energy for cells and also a prime donor of nitrogen in the biosynthesis of many amino acids. Several studies have advocated the role of glutamic acid in cancer therapy. Identification of metabolic signatures in cancer cells will be crucial for advancement of cancer therapies based on the cell’s metabolic state. Stable nitrogen isotope ratios (15N/14N, δ15N) are of particular advantage to understand the metabolic state of cancer cells, since most biochemical reactions involve transfer of nitrogen. In our study, we used the natural abundances of nitrogen isotopes (δ15N values) of individual amino acids from human colorectal cancer cell lines to investigate isotope discrimination among amino acids. Significant effects were noticed in the case of glutamic acid, alanine, aspartic acid and proline between cancer and healthy cells. The data suggest that glutamic acid is a nitrogen acceptor while alanine, aspartic acid and proline are nitrogen donors in cancerous cells. One plausible explanation is the transamination of the three acids to produce glutamic acid in cancerous cells.R. V. KrishnamurthyYogesh R. SuryawanshiKarim EssaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q R. V. Krishnamurthy Yogesh R. Suryawanshi Karim Essani Nitrogen isotopes provide clues to amino acid metabolism in human colorectal cancer cells
description	Abstract Glutamic acid and alanine make up more than 60 per cent of the total amino acids in the human body. Glutamine is a significant source of energy for cells and also a prime donor of nitrogen in the biosynthesis of many amino acids. Several studies have advocated the role of glutamic acid in cancer therapy. Identification of metabolic signatures in cancer cells will be crucial for advancement of cancer therapies based on the cell’s metabolic state. Stable nitrogen isotope ratios (15N/14N, δ15N) are of particular advantage to understand the metabolic state of cancer cells, since most biochemical reactions involve transfer of nitrogen. In our study, we used the natural abundances of nitrogen isotopes (δ15N values) of individual amino acids from human colorectal cancer cell lines to investigate isotope discrimination among amino acids. Significant effects were noticed in the case of glutamic acid, alanine, aspartic acid and proline between cancer and healthy cells. The data suggest that glutamic acid is a nitrogen acceptor while alanine, aspartic acid and proline are nitrogen donors in cancerous cells. One plausible explanation is the transamination of the three acids to produce glutamic acid in cancerous cells.
format	article
author	R. V. Krishnamurthy Yogesh R. Suryawanshi Karim Essani
author_facet	R. V. Krishnamurthy Yogesh R. Suryawanshi Karim Essani
author_sort	R. V. Krishnamurthy
title	Nitrogen isotopes provide clues to amino acid metabolism in human colorectal cancer cells
title_short	Nitrogen isotopes provide clues to amino acid metabolism in human colorectal cancer cells
title_full	Nitrogen isotopes provide clues to amino acid metabolism in human colorectal cancer cells
title_fullStr	Nitrogen isotopes provide clues to amino acid metabolism in human colorectal cancer cells
title_full_unstemmed	Nitrogen isotopes provide clues to amino acid metabolism in human colorectal cancer cells
title_sort	nitrogen isotopes provide clues to amino acid metabolism in human colorectal cancer cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f062f5b9c7f74cbbb9dccf10520e6f50
work_keys_str_mv	AT rvkrishnamurthy nitrogenisotopesprovidecluestoaminoacidmetabolisminhumancolorectalcancercells AT yogeshrsuryawanshi nitrogenisotopesprovidecluestoaminoacidmetabolisminhumancolorectalcancercells AT karimessani nitrogenisotopesprovidecluestoaminoacidmetabolisminhumancolorectalcancercells
_version_	1718388553963012096

Nitrogen isotopes provide clues to amino acid metabolism in human colorectal cancer cells

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