Effects of annatto-derived tocotrienol supplementation on osteoporosis induced by testosterone deficiency in rats

Kok-Yong Chin, Soelaiman Ima-Nirwana Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia Background: Previous animal models have demonstrated that tocotrienol is a potential treatment for postmenopausal osteoporosis. This study evaluated the anti...

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Autores principales:	Chin KY, Ima-Nirwana S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Bone remodeling Osteoporosis Testosterone Tocotrienol Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/f064b1e21ef74a2fa3cf2e8440596229
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spelling	oai:doaj.org-article:f064b1e21ef74a2fa3cf2e84405962292021-12-02T07:04:43ZEffects of annatto-derived tocotrienol supplementation on osteoporosis induced by testosterone deficiency in rats1178-1998https://doaj.org/article/f064b1e21ef74a2fa3cf2e84405962292014-08-01T00:00:00Zhttps://www.dovepress.com/effects-of-annatto-derived-tocotrienol-supplementation-on-osteoporosis-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Kok-Yong Chin, Soelaiman Ima-Nirwana Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia Background: Previous animal models have demonstrated that tocotrienol is a potential treatment for postmenopausal osteoporosis. This study evaluated the antiosteoporotic effects of annatto-derived tocotrienol (AnTT) using a testosterone-deficient osteoporotic rat model. Methods: Forty rats were divided randomly into baseline, sham, orchidectomized, AnTT, and testosterone groups. The baseline group was euthanized without undergoing any surgical treatment or intervention. The remaining groups underwent orchidectomy, with the exception of the sham group. AnTT 60 mg/kg/day was given orally to the AnTT group, while the testosterone group received testosterone enanthate 7 mg/kg per week intramuscularly for 8 weeks. Structural changes in trabecular bone at the proximal tibia were examined using microcomputed tomography. Structural and dynamic changes at the distal femur were examined using histomorphometric methods. Serum osteocalcin and C-terminal of type 1 collagen crosslinks were measured. Bone-related gene expression in the distal femur was examined. Results: There were significant degenerative changes in structural indices in the orchidectomized group (P<0.05), but no significant changes in dynamic indices, bone remodeling markers, or gene expression (P>0.05) when compared with the sham group. The AnTT group showed significant improvement in structural indices at the femur (P<0.05) and significantly increased expression of bone formation genes (P<0.05). Testosterone was more effective than AnTT in preventing degeneration of bone structural indices in the femur and tibia (P<0.05). Conclusion: AnTT supplementation improves bone health in testosterone-deficient rats by enhancing bone formation. Its potential should be evaluated further by varying the dosage and treatment duration. Keywords: bone remodeling, osteoporosis, testosterone, tocotrienolChin KYIma-Nirwana SDove Medical PressarticleBone remodelingOsteoporosisTestosteroneTocotrienolGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 9, Pp 1247-1259 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bone remodeling Osteoporosis Testosterone Tocotrienol Geriatrics RC952-954.6
spellingShingle	Bone remodeling Osteoporosis Testosterone Tocotrienol Geriatrics RC952-954.6 Chin KY Ima-Nirwana S Effects of annatto-derived tocotrienol supplementation on osteoporosis induced by testosterone deficiency in rats
description	Kok-Yong Chin, Soelaiman Ima-Nirwana Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia Background: Previous animal models have demonstrated that tocotrienol is a potential treatment for postmenopausal osteoporosis. This study evaluated the antiosteoporotic effects of annatto-derived tocotrienol (AnTT) using a testosterone-deficient osteoporotic rat model. Methods: Forty rats were divided randomly into baseline, sham, orchidectomized, AnTT, and testosterone groups. The baseline group was euthanized without undergoing any surgical treatment or intervention. The remaining groups underwent orchidectomy, with the exception of the sham group. AnTT 60 mg/kg/day was given orally to the AnTT group, while the testosterone group received testosterone enanthate 7 mg/kg per week intramuscularly for 8 weeks. Structural changes in trabecular bone at the proximal tibia were examined using microcomputed tomography. Structural and dynamic changes at the distal femur were examined using histomorphometric methods. Serum osteocalcin and C-terminal of type 1 collagen crosslinks were measured. Bone-related gene expression in the distal femur was examined. Results: There were significant degenerative changes in structural indices in the orchidectomized group (P<0.05), but no significant changes in dynamic indices, bone remodeling markers, or gene expression (P>0.05) when compared with the sham group. The AnTT group showed significant improvement in structural indices at the femur (P<0.05) and significantly increased expression of bone formation genes (P<0.05). Testosterone was more effective than AnTT in preventing degeneration of bone structural indices in the femur and tibia (P<0.05). Conclusion: AnTT supplementation improves bone health in testosterone-deficient rats by enhancing bone formation. Its potential should be evaluated further by varying the dosage and treatment duration. Keywords: bone remodeling, osteoporosis, testosterone, tocotrienol
format	article
author	Chin KY Ima-Nirwana S
author_facet	Chin KY Ima-Nirwana S
author_sort	Chin KY
title	Effects of annatto-derived tocotrienol supplementation on osteoporosis induced by testosterone deficiency in rats
title_short	Effects of annatto-derived tocotrienol supplementation on osteoporosis induced by testosterone deficiency in rats
title_full	Effects of annatto-derived tocotrienol supplementation on osteoporosis induced by testosterone deficiency in rats
title_fullStr	Effects of annatto-derived tocotrienol supplementation on osteoporosis induced by testosterone deficiency in rats
title_full_unstemmed	Effects of annatto-derived tocotrienol supplementation on osteoporosis induced by testosterone deficiency in rats
title_sort	effects of annatto-derived tocotrienol supplementation on osteoporosis induced by testosterone deficiency in rats
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/f064b1e21ef74a2fa3cf2e8440596229
work_keys_str_mv	AT chinky effectsofannattoderivedtocotrienolsupplementationonosteoporosisinducedbytestosteronedeficiencyinrats AT imanirwanas effectsofannattoderivedtocotrienolsupplementationonosteoporosisinducedbytestosteronedeficiencyinrats
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Effects of annatto-derived tocotrienol supplementation on osteoporosis induced by testosterone deficiency in rats

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