Comparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models

Abstract Background Osteoarthritis (OA) is a multifaceted condition that affects both the subchondral bones and the articular cartilage. Animal models are widely used as an effective supplement and simulation for human OA studies in investigating disease mechanisms and pathophysiology. This study is...

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Autores principales:	Sharifah Zakiah Syed Sulaiman, Wei Miao Tan, Rozanaliza Radzi, Intan Nur Fatiha Shafie, Mokrish Ajat, Rozaihan Mansor, Suhaila Mohamed, Angela Min Hwei Ng, Seng Fong Lau
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Osteoarthritis (OA) Subchondral bone Articular cartilage Micro-computed tomography (micro-CT) Histology Anterior cruciate ligament transection (ACLT) Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935
Acceso en línea:	https://doaj.org/article/f0764e3fda644ed692066266c2dd52e6
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spelling	oai:doaj.org-article:f0764e3fda644ed692066266c2dd52e62021-11-14T12:29:40ZComparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models10.1186/s13018-021-02781-z1749-799Xhttps://doaj.org/article/f0764e3fda644ed692066266c2dd52e62021-11-01T00:00:00Zhttps://doi.org/10.1186/s13018-021-02781-zhttps://doaj.org/toc/1749-799XAbstract Background Osteoarthritis (OA) is a multifaceted condition that affects both the subchondral bones and the articular cartilage. Animal models are widely used as an effective supplement and simulation for human OA studies in investigating disease mechanisms and pathophysiology. This study is aimed to evaluate the temporal changes of bone and cartilage in surgically and chemically induced osteoarthritis using micro-computed tomography and histology. Methods Thirty rabbits underwent either anterior cruciate ligament transection (ACLT) procedure or injected intraarticularly with monosodium iodoacetate (MIA, 8 mg) at the right knee joint. The subchondral bones were scanned via micro-CT, and articular cartilage was assessed histologically at 4-, 8- and 12-week post-induction. Results Based on bone micro-architecture parameters, the surgically induced group revealed bone remodelling processes, indicated by increase bone volume, thickening of trabeculae, reduced trabecular separation and reduced porosity. On the other hand, the chemically induced group showed active bone resorption processes depicted by decrease bone volume, thinning of trabeculae, increased separation of trabecular and increased porosity consistently until week 12. Histologically, the chemically induced group showed more severe articular cartilage damage compared to the surgically induced group. Conclusions It can be concluded that in the ACLT group, subchondral bone remodelling precedes articular cartilage damage and vice versa in the MIA group. The findings revealed distinct pathogenic pathways for both induction methods, providing insight into tailored therapeutic strategies, as well as disease progression and treatment outcomes monitoring.Sharifah Zakiah Syed SulaimanWei Miao TanRozanaliza RadziIntan Nur Fatiha ShafieMokrish AjatRozaihan MansorSuhaila MohamedAngela Min Hwei NgSeng Fong LauBMCarticleOsteoarthritis (OA)Subchondral boneArticular cartilageMicro-computed tomography (micro-CT)HistologyAnterior cruciate ligament transection (ACLT)Orthopedic surgeryRD701-811Diseases of the musculoskeletal systemRC925-935ENJournal of Orthopaedic Surgery and Research, Vol 16, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osteoarthritis (OA) Subchondral bone Articular cartilage Micro-computed tomography (micro-CT) Histology Anterior cruciate ligament transection (ACLT) Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935
spellingShingle	Osteoarthritis (OA) Subchondral bone Articular cartilage Micro-computed tomography (micro-CT) Histology Anterior cruciate ligament transection (ACLT) Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935 Sharifah Zakiah Syed Sulaiman Wei Miao Tan Rozanaliza Radzi Intan Nur Fatiha Shafie Mokrish Ajat Rozaihan Mansor Suhaila Mohamed Angela Min Hwei Ng Seng Fong Lau Comparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models
description	Abstract Background Osteoarthritis (OA) is a multifaceted condition that affects both the subchondral bones and the articular cartilage. Animal models are widely used as an effective supplement and simulation for human OA studies in investigating disease mechanisms and pathophysiology. This study is aimed to evaluate the temporal changes of bone and cartilage in surgically and chemically induced osteoarthritis using micro-computed tomography and histology. Methods Thirty rabbits underwent either anterior cruciate ligament transection (ACLT) procedure or injected intraarticularly with monosodium iodoacetate (MIA, 8 mg) at the right knee joint. The subchondral bones were scanned via micro-CT, and articular cartilage was assessed histologically at 4-, 8- and 12-week post-induction. Results Based on bone micro-architecture parameters, the surgically induced group revealed bone remodelling processes, indicated by increase bone volume, thickening of trabeculae, reduced trabecular separation and reduced porosity. On the other hand, the chemically induced group showed active bone resorption processes depicted by decrease bone volume, thinning of trabeculae, increased separation of trabecular and increased porosity consistently until week 12. Histologically, the chemically induced group showed more severe articular cartilage damage compared to the surgically induced group. Conclusions It can be concluded that in the ACLT group, subchondral bone remodelling precedes articular cartilage damage and vice versa in the MIA group. The findings revealed distinct pathogenic pathways for both induction methods, providing insight into tailored therapeutic strategies, as well as disease progression and treatment outcomes monitoring.
format	article
author	Sharifah Zakiah Syed Sulaiman Wei Miao Tan Rozanaliza Radzi Intan Nur Fatiha Shafie Mokrish Ajat Rozaihan Mansor Suhaila Mohamed Angela Min Hwei Ng Seng Fong Lau
author_facet	Sharifah Zakiah Syed Sulaiman Wei Miao Tan Rozanaliza Radzi Intan Nur Fatiha Shafie Mokrish Ajat Rozaihan Mansor Suhaila Mohamed Angela Min Hwei Ng Seng Fong Lau
author_sort	Sharifah Zakiah Syed Sulaiman
title	Comparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models
title_short	Comparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models
title_full	Comparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models
title_fullStr	Comparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models
title_full_unstemmed	Comparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models
title_sort	comparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models
publisher	BMC
publishDate	2021
url	https://doaj.org/article/f0764e3fda644ed692066266c2dd52e6
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Comparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models

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