Subversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.

Invading bacteria are recognized, captured and killed by a specialized form of autophagy, called xenophagy. Recently, defects in xenophagy in Crohn's disease (CD) have been implicated in the pathogenesis of human chronic inflammatory diseases of uncertain etiology of the gastrointestinal tract....

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Autores principales:	Abderrahman Chargui, Annabelle Cesaro, Sanda Mimouna, Mohamed Fareh, Patrick Brest, Philippe Naquet, Arlette Darfeuille-Michaud, Xavier Hébuterne, Baharia Mograbi, Valérie Vouret-Craviari, Paul Hofman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f076d6596489462884117374c7efc47d
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spelling	oai:doaj.org-article:f076d6596489462884117374c7efc47d2021-11-18T08:04:54ZSubversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.1932-620310.1371/journal.pone.0051727https://doaj.org/article/f076d6596489462884117374c7efc47d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23272151/?tool=EBIhttps://doaj.org/toc/1932-6203Invading bacteria are recognized, captured and killed by a specialized form of autophagy, called xenophagy. Recently, defects in xenophagy in Crohn's disease (CD) have been implicated in the pathogenesis of human chronic inflammatory diseases of uncertain etiology of the gastrointestinal tract. We show here that pathogenic adherent-invasive Escherichia coli (AIEC) isolated from CD patients are able to adhere and invade neutrophils, which represent the first line of defense against bacteria. Of particular interest, AIEC infection of neutrophil-like PLB-985 cells blocked autophagy at the autolysosomal step, which allowed intracellular survival of bacteria and exacerbated interleukin-8 (IL-8) production. Interestingly, this block in autophagy correlated with the induction of autophagic cell death. Likewise, stimulation of autophagy by nutrient starvation or rapamycin treatment reduced intracellular AIEC survival and IL-8 production. Finally, treatment with an inhibitor of autophagy decreased cell death of AIEC-infected neutrophil-like PLB-985 cells. In conclusion, excessive autophagy in AIEC infection triggered cell death of neutrophils.Abderrahman CharguiAnnabelle CesaroSanda MimounaMohamed FarehPatrick BrestPhilippe NaquetArlette Darfeuille-MichaudXavier HébuterneBaharia MograbiValérie Vouret-CraviariPaul HofmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51727 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abderrahman Chargui Annabelle Cesaro Sanda Mimouna Mohamed Fareh Patrick Brest Philippe Naquet Arlette Darfeuille-Michaud Xavier Hébuterne Baharia Mograbi Valérie Vouret-Craviari Paul Hofman Subversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.
description	Invading bacteria are recognized, captured and killed by a specialized form of autophagy, called xenophagy. Recently, defects in xenophagy in Crohn's disease (CD) have been implicated in the pathogenesis of human chronic inflammatory diseases of uncertain etiology of the gastrointestinal tract. We show here that pathogenic adherent-invasive Escherichia coli (AIEC) isolated from CD patients are able to adhere and invade neutrophils, which represent the first line of defense against bacteria. Of particular interest, AIEC infection of neutrophil-like PLB-985 cells blocked autophagy at the autolysosomal step, which allowed intracellular survival of bacteria and exacerbated interleukin-8 (IL-8) production. Interestingly, this block in autophagy correlated with the induction of autophagic cell death. Likewise, stimulation of autophagy by nutrient starvation or rapamycin treatment reduced intracellular AIEC survival and IL-8 production. Finally, treatment with an inhibitor of autophagy decreased cell death of AIEC-infected neutrophil-like PLB-985 cells. In conclusion, excessive autophagy in AIEC infection triggered cell death of neutrophils.
format	article
author	Abderrahman Chargui Annabelle Cesaro Sanda Mimouna Mohamed Fareh Patrick Brest Philippe Naquet Arlette Darfeuille-Michaud Xavier Hébuterne Baharia Mograbi Valérie Vouret-Craviari Paul Hofman
author_facet	Abderrahman Chargui Annabelle Cesaro Sanda Mimouna Mohamed Fareh Patrick Brest Philippe Naquet Arlette Darfeuille-Michaud Xavier Hébuterne Baharia Mograbi Valérie Vouret-Craviari Paul Hofman
author_sort	Abderrahman Chargui
title	Subversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.
title_short	Subversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.
title_full	Subversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.
title_fullStr	Subversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.
title_full_unstemmed	Subversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.
title_sort	subversion of autophagy in adherent invasive escherichia coli-infected neutrophils induces inflammation and cell death.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f076d6596489462884117374c7efc47d
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Subversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.

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