Pig embryonic stem cell line with porcine-specific OCT4 upstream region based dual reporter system

Here, we report pig embryonic stem cells (ESCs) originating from parthenogenetic blastocysts which were developed from 2-cell embryos micro-injected with porcine OCT4 reporter system cultured in previous reported chemically defined culture media. The ESCs with reporter system expressed pluripotency...

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Autores principales:	Seung-Hun Kim, Kwang-Hwan Choi, Jinsol Jeong, Mingyun Lee, Dong-Kyung Lee, Jong-Nam Oh, Gyeong Cheol Choe, Du-Min Go, Dae-Yong Kim, Chang-Kyu Lee
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f078c415f4b443b8bde8dcd85c315741
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spelling	oai:doaj.org-article:f078c415f4b443b8bde8dcd85c3157412021-12-02T05:00:18ZPig embryonic stem cell line with porcine-specific OCT4 upstream region based dual reporter system1873-506110.1016/j.scr.2021.102609https://doaj.org/article/f078c415f4b443b8bde8dcd85c3157412021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1873506121004566https://doaj.org/toc/1873-5061Here, we report pig embryonic stem cells (ESCs) originating from parthenogenetic blastocysts which were developed from 2-cell embryos micro-injected with porcine OCT4 reporter system cultured in previous reported chemically defined culture media. The ESCs with reporter system expressed pluripotency markers and fluorescent signals produced by OCT4 reporter system. Also, they were capable of forming teratomas following subcutaneous injection into nude mice. Since reporter system enables the non-destructive classification of the condition of live pluripotent stem cells, this reporter cell line could be a useful resource for research on species-specific pluripotency.Seung-Hun KimKwang-Hwan ChoiJinsol JeongMingyun LeeDong-Kyung LeeJong-Nam OhGyeong Cheol ChoeDu-Min GoDae-Yong KimChang-Kyu LeeElsevierarticleBiology (General)QH301-705.5ENStem Cell Research, Vol 57, Iss , Pp 102609- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Seung-Hun Kim Kwang-Hwan Choi Jinsol Jeong Mingyun Lee Dong-Kyung Lee Jong-Nam Oh Gyeong Cheol Choe Du-Min Go Dae-Yong Kim Chang-Kyu Lee Pig embryonic stem cell line with porcine-specific OCT4 upstream region based dual reporter system
description	Here, we report pig embryonic stem cells (ESCs) originating from parthenogenetic blastocysts which were developed from 2-cell embryos micro-injected with porcine OCT4 reporter system cultured in previous reported chemically defined culture media. The ESCs with reporter system expressed pluripotency markers and fluorescent signals produced by OCT4 reporter system. Also, they were capable of forming teratomas following subcutaneous injection into nude mice. Since reporter system enables the non-destructive classification of the condition of live pluripotent stem cells, this reporter cell line could be a useful resource for research on species-specific pluripotency.
format	article
author	Seung-Hun Kim Kwang-Hwan Choi Jinsol Jeong Mingyun Lee Dong-Kyung Lee Jong-Nam Oh Gyeong Cheol Choe Du-Min Go Dae-Yong Kim Chang-Kyu Lee
author_facet	Seung-Hun Kim Kwang-Hwan Choi Jinsol Jeong Mingyun Lee Dong-Kyung Lee Jong-Nam Oh Gyeong Cheol Choe Du-Min Go Dae-Yong Kim Chang-Kyu Lee
author_sort	Seung-Hun Kim
title	Pig embryonic stem cell line with porcine-specific OCT4 upstream region based dual reporter system
title_short	Pig embryonic stem cell line with porcine-specific OCT4 upstream region based dual reporter system
title_full	Pig embryonic stem cell line with porcine-specific OCT4 upstream region based dual reporter system
title_fullStr	Pig embryonic stem cell line with porcine-specific OCT4 upstream region based dual reporter system
title_full_unstemmed	Pig embryonic stem cell line with porcine-specific OCT4 upstream region based dual reporter system
title_sort	pig embryonic stem cell line with porcine-specific oct4 upstream region based dual reporter system
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f078c415f4b443b8bde8dcd85c315741
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Pig embryonic stem cell line with porcine-specific OCT4 upstream region based dual reporter system

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