Innate immune responses are increased in chronic obstructive pulmonary disease.

<h4>Background</h4>Chronic obstructive pulmonary disease (COPD) is characterised by irreversible airflow obstruction, neutrophilic airway inflammation and chronic bacterial colonisation, however the role of the innate immune response in the pathogenesis of COPD remains unclear.<h4>...

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Autores principales:	Katherine Joanne Baines, Jodie Louise Simpson, Peter Gerard Gibson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f07fb75d7d44430f9d001cf9f7762f43
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spelling	oai:doaj.org-article:f07fb75d7d44430f9d001cf9f7762f432021-11-18T06:56:29ZInnate immune responses are increased in chronic obstructive pulmonary disease.1932-620310.1371/journal.pone.0018426https://doaj.org/article/f07fb75d7d44430f9d001cf9f7762f432011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21483784/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Chronic obstructive pulmonary disease (COPD) is characterised by irreversible airflow obstruction, neutrophilic airway inflammation and chronic bacterial colonisation, however the role of the innate immune response in the pathogenesis of COPD remains unclear.<h4>Methods</h4>Induced sputum was obtained from adults with COPD (n=22), and healthy controls (n=29) and was processed for differential cell counts. The sputum supernatant was assayed for innate immune mediators using ELISA, whilst sputum gene expression was measured using real-time PCR. Peripheral blood neutrophils were isolated and their response to lipopolysaccaride (LPS) stimulation was assessed in a subgroup of participants with COPD (n=13) and healthy controls (n=21).<h4>Results</h4>Participants with COPD had significantly higher protein levels of interleukin (IL)-8, and neutrophil elastase (NE) and detection of oncostatin M (OSM) compared to healthy controls. Gene expression for toll-like receptor (TLR) 2, IL-8 and OSM were also significantly higher in COPD participants. The level of IL-1β, surfactant protein (SP)-A, matrix metalloproteinase (MMP)-9 and TLR4 mRNA was not significantly different between groups. The level of innate immune response markers were highly associated with the presence of sputum neutrophils, each other and the degree of airflow limitation (FEV1/FVC). Peripheral blood neutrophils from participants with COPD had an increased response to stimulation by LPS; with a greater fold increase in the production of IL-8 and MMP-9 protein, and gene expression of IL-8, TLR2 and TLR4.<h4>Conclusions</h4>The innate immune response is increased in the airways and circulating neutrophils in COPD, and may be an important mechanism involved in disease pathogenesis.Katherine Joanne BainesJodie Louise SimpsonPeter Gerard GibsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e18426 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katherine Joanne Baines Jodie Louise Simpson Peter Gerard Gibson Innate immune responses are increased in chronic obstructive pulmonary disease.
description	<h4>Background</h4>Chronic obstructive pulmonary disease (COPD) is characterised by irreversible airflow obstruction, neutrophilic airway inflammation and chronic bacterial colonisation, however the role of the innate immune response in the pathogenesis of COPD remains unclear.<h4>Methods</h4>Induced sputum was obtained from adults with COPD (n=22), and healthy controls (n=29) and was processed for differential cell counts. The sputum supernatant was assayed for innate immune mediators using ELISA, whilst sputum gene expression was measured using real-time PCR. Peripheral blood neutrophils were isolated and their response to lipopolysaccaride (LPS) stimulation was assessed in a subgroup of participants with COPD (n=13) and healthy controls (n=21).<h4>Results</h4>Participants with COPD had significantly higher protein levels of interleukin (IL)-8, and neutrophil elastase (NE) and detection of oncostatin M (OSM) compared to healthy controls. Gene expression for toll-like receptor (TLR) 2, IL-8 and OSM were also significantly higher in COPD participants. The level of IL-1β, surfactant protein (SP)-A, matrix metalloproteinase (MMP)-9 and TLR4 mRNA was not significantly different between groups. The level of innate immune response markers were highly associated with the presence of sputum neutrophils, each other and the degree of airflow limitation (FEV1/FVC). Peripheral blood neutrophils from participants with COPD had an increased response to stimulation by LPS; with a greater fold increase in the production of IL-8 and MMP-9 protein, and gene expression of IL-8, TLR2 and TLR4.<h4>Conclusions</h4>The innate immune response is increased in the airways and circulating neutrophils in COPD, and may be an important mechanism involved in disease pathogenesis.
format	article
author	Katherine Joanne Baines Jodie Louise Simpson Peter Gerard Gibson
author_facet	Katherine Joanne Baines Jodie Louise Simpson Peter Gerard Gibson
author_sort	Katherine Joanne Baines
title	Innate immune responses are increased in chronic obstructive pulmonary disease.
title_short	Innate immune responses are increased in chronic obstructive pulmonary disease.
title_full	Innate immune responses are increased in chronic obstructive pulmonary disease.
title_fullStr	Innate immune responses are increased in chronic obstructive pulmonary disease.
title_full_unstemmed	Innate immune responses are increased in chronic obstructive pulmonary disease.
title_sort	innate immune responses are increased in chronic obstructive pulmonary disease.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f07fb75d7d44430f9d001cf9f7762f43
work_keys_str_mv	AT katherinejoannebaines innateimmuneresponsesareincreasedinchronicobstructivepulmonarydisease AT jodielouisesimpson innateimmuneresponsesareincreasedinchronicobstructivepulmonarydisease AT petergerardgibson innateimmuneresponsesareincreasedinchronicobstructivepulmonarydisease
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Innate immune responses are increased in chronic obstructive pulmonary disease.

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