Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells

Human mesenchymal stem cells can differentiate into various cell types and are useful for applications in regenerative medicine. Previous studies indicated that dental pulp exfoliated from deciduous teeth is a valuable alternative for dental tissue engineering because it contains stem cells with a r...

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Autores principales:	Masae Okuno, Sho Aoki, Saki Kawai, Rie Imataki, Yoko Abe, Kyoko Harada, Kenji Arita
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	non-thermal atmospheric pressure plasma (NTAPP) human deciduous dental pulp fibroblast-like cells regenerative medicine Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/f082595ba1d6426d90fdcaaa1d370f7b
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spelling	oai:doaj.org-article:f082595ba1d6426d90fdcaaa1d370f7b2021-11-11T15:10:51ZEffect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells10.3390/app1121101192076-3417https://doaj.org/article/f082595ba1d6426d90fdcaaa1d370f7b2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/10119https://doaj.org/toc/2076-3417Human mesenchymal stem cells can differentiate into various cell types and are useful for applications in regenerative medicine. Previous studies indicated that dental pulp exfoliated from deciduous teeth is a valuable alternative for dental tissue engineering because it contains stem cells with a relatively high proliferation rate. For clinical application, it is necessary to rapidly obtain a sufficient number of cells in vitro and maintain their undifferentiated state; however, the abundance of stem cells in the dental pulp tissue is limited. Non-thermal atmospheric pressure plasma (NTAPP) has been applied in regenerative medicine because it activates cell proliferation. Here, we examined the effects of NTAPP to activate the proliferation of human deciduous dental pulp fibroblast-like cells (hDDPFs) in vitro. Compared with untreated cells, NTAPP increased cell proliferation by 1.3-fold, significantly upregulated well-known pluripotent genes for stemness (e.g., <i>Oct4</i>, <i>Sox2</i>, and <i>Nanog</i>), and activated the expression of stem cell-specific surface markers (e.g., CD105). Overall, NTAPP activated the proliferation of various mesodermal-derived human adult stem cells while maintaining their pluripotency and stemness. In conclusion, NTAPP is a potential tool to expand the population of various adult stem cells in vitro for medical applications.Masae OkunoSho AokiSaki KawaiRie ImatakiYoko AbeKyoko HaradaKenji AritaMDPI AGarticlenon-thermal atmospheric pressure plasma (NTAPP)human deciduous dental pulp fibroblast-like cellsregenerative medicineTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10119, p 10119 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	non-thermal atmospheric pressure plasma (NTAPP) human deciduous dental pulp fibroblast-like cells regenerative medicine Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	non-thermal atmospheric pressure plasma (NTAPP) human deciduous dental pulp fibroblast-like cells regenerative medicine Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Masae Okuno Sho Aoki Saki Kawai Rie Imataki Yoko Abe Kyoko Harada Kenji Arita Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells
description	Human mesenchymal stem cells can differentiate into various cell types and are useful for applications in regenerative medicine. Previous studies indicated that dental pulp exfoliated from deciduous teeth is a valuable alternative for dental tissue engineering because it contains stem cells with a relatively high proliferation rate. For clinical application, it is necessary to rapidly obtain a sufficient number of cells in vitro and maintain their undifferentiated state; however, the abundance of stem cells in the dental pulp tissue is limited. Non-thermal atmospheric pressure plasma (NTAPP) has been applied in regenerative medicine because it activates cell proliferation. Here, we examined the effects of NTAPP to activate the proliferation of human deciduous dental pulp fibroblast-like cells (hDDPFs) in vitro. Compared with untreated cells, NTAPP increased cell proliferation by 1.3-fold, significantly upregulated well-known pluripotent genes for stemness (e.g., <i>Oct4</i>, <i>Sox2</i>, and <i>Nanog</i>), and activated the expression of stem cell-specific surface markers (e.g., CD105). Overall, NTAPP activated the proliferation of various mesodermal-derived human adult stem cells while maintaining their pluripotency and stemness. In conclusion, NTAPP is a potential tool to expand the population of various adult stem cells in vitro for medical applications.
format	article
author	Masae Okuno Sho Aoki Saki Kawai Rie Imataki Yoko Abe Kyoko Harada Kenji Arita
author_facet	Masae Okuno Sho Aoki Saki Kawai Rie Imataki Yoko Abe Kyoko Harada Kenji Arita
author_sort	Masae Okuno
title	Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells
title_short	Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells
title_full	Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells
title_fullStr	Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells
title_full_unstemmed	Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells
title_sort	effect of non-thermal atmospheric pressure plasma on differentiation potential of human deciduous dental pulp fibroblast-like cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f082595ba1d6426d90fdcaaa1d370f7b
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Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells

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