Dorso-ventral heterogeneity in tracheal basal stem cells

The tracheal basal cells (BCs) function as stem cells to maintain the epithelium in steady state and repair it after injury. The airway is surrounded by cartilage ventrolaterally and smooth muscle dorsally. Lineage tracing using Krt5-CreER shows dorsal BCs produce more, larger, clones than ventral B...

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Autores principales:	Tomomi Tadokoro, Keisuke Tanaka, Shun Osakabe, Mimoko Kato, Hisato Kobayashi, Brigid L. M. Hogan, Hideki Taniguchi
Formato:	article
Lenguaje:	EN
Publicado:	The Company of Biologists 2021
Materias:	basal stem cells tracheal epithelium dorso-ventral difference basal cell heterogeneity Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f087c54da9214d3bb4983904daed81dc
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spelling	oai:doaj.org-article:f087c54da9214d3bb4983904daed81dc2021-11-28T16:01:10ZDorso-ventral heterogeneity in tracheal basal stem cells2046-639010.1242/bio.058676https://doaj.org/article/f087c54da9214d3bb4983904daed81dc2021-09-01T00:00:00Zhttp://bio.biologists.org/content/10/9/bio058676https://doaj.org/toc/2046-6390The tracheal basal cells (BCs) function as stem cells to maintain the epithelium in steady state and repair it after injury. The airway is surrounded by cartilage ventrolaterally and smooth muscle dorsally. Lineage tracing using Krt5-CreER shows dorsal BCs produce more, larger, clones than ventral BCs. Large clones were found between cartilage and smooth muscle where subpopulation of dorsal BCs exists. Three-dimensional organoid culture of BCs demonstrated that dorsal BCs show higher colony forming efficacy to ventral BCs. Gene ontology analysis revealed that genes expressed in dorsal BCs are enriched in wound healing while ventral BCs are enriched in response to external stimulus and immune response. Significantly, ventral BCs express Myostatin, which inhibits the growth of smooth muscle cells, and HGF, which facilitates cartilage repair. The results support the hypothesis that BCs from the dorso-ventral airways have intrinsic molecular and behavioural differences relevant to their in vivo function.Tomomi TadokoroKeisuke TanakaShun OsakabeMimoko KatoHisato KobayashiBrigid L. M. HoganHideki TaniguchiThe Company of Biologistsarticlebasal stem cellstracheal epitheliumdorso-ventral differencebasal cell heterogeneityScienceQBiology (General)QH301-705.5ENBiology Open, Vol 10, Iss 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	basal stem cells tracheal epithelium dorso-ventral difference basal cell heterogeneity Science Q Biology (General) QH301-705.5
spellingShingle	basal stem cells tracheal epithelium dorso-ventral difference basal cell heterogeneity Science Q Biology (General) QH301-705.5 Tomomi Tadokoro Keisuke Tanaka Shun Osakabe Mimoko Kato Hisato Kobayashi Brigid L. M. Hogan Hideki Taniguchi Dorso-ventral heterogeneity in tracheal basal stem cells
description	The tracheal basal cells (BCs) function as stem cells to maintain the epithelium in steady state and repair it after injury. The airway is surrounded by cartilage ventrolaterally and smooth muscle dorsally. Lineage tracing using Krt5-CreER shows dorsal BCs produce more, larger, clones than ventral BCs. Large clones were found between cartilage and smooth muscle where subpopulation of dorsal BCs exists. Three-dimensional organoid culture of BCs demonstrated that dorsal BCs show higher colony forming efficacy to ventral BCs. Gene ontology analysis revealed that genes expressed in dorsal BCs are enriched in wound healing while ventral BCs are enriched in response to external stimulus and immune response. Significantly, ventral BCs express Myostatin, which inhibits the growth of smooth muscle cells, and HGF, which facilitates cartilage repair. The results support the hypothesis that BCs from the dorso-ventral airways have intrinsic molecular and behavioural differences relevant to their in vivo function.
format	article
author	Tomomi Tadokoro Keisuke Tanaka Shun Osakabe Mimoko Kato Hisato Kobayashi Brigid L. M. Hogan Hideki Taniguchi
author_facet	Tomomi Tadokoro Keisuke Tanaka Shun Osakabe Mimoko Kato Hisato Kobayashi Brigid L. M. Hogan Hideki Taniguchi
author_sort	Tomomi Tadokoro
title	Dorso-ventral heterogeneity in tracheal basal stem cells
title_short	Dorso-ventral heterogeneity in tracheal basal stem cells
title_full	Dorso-ventral heterogeneity in tracheal basal stem cells
title_fullStr	Dorso-ventral heterogeneity in tracheal basal stem cells
title_full_unstemmed	Dorso-ventral heterogeneity in tracheal basal stem cells
title_sort	dorso-ventral heterogeneity in tracheal basal stem cells
publisher	The Company of Biologists
publishDate	2021
url	https://doaj.org/article/f087c54da9214d3bb4983904daed81dc
work_keys_str_mv	AT tomomitadokoro dorsoventralheterogeneityintrachealbasalstemcells AT keisuketanaka dorsoventralheterogeneityintrachealbasalstemcells AT shunosakabe dorsoventralheterogeneityintrachealbasalstemcells AT mimokokato dorsoventralheterogeneityintrachealbasalstemcells AT hisatokobayashi dorsoventralheterogeneityintrachealbasalstemcells AT brigidlmhogan dorsoventralheterogeneityintrachealbasalstemcells AT hidekitaniguchi dorsoventralheterogeneityintrachealbasalstemcells
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Dorso-ventral heterogeneity in tracheal basal stem cells

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