Fatty acid profile and unigene-derived simple sequence repeat markers in tung tree (Vernicia fordii).

Tung tree (Vernicia fordii) provides the sole source of tung oil widely used in industry. Lack of fatty acid composition and molecular markers hinders biochemical, genetic and breeding research. The objectives of this study were to determine fatty acid profiles and develop unigene-derived simple seq...

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Autores principales:	Lin Zhang, Baoguang Jia, Xiaofeng Tan, Chandra S Thammina, Hongxu Long, Min Liu, Shanna Wen, Xianliang Song, Heping Cao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f087fa0e992a403aad99c93c50bc6d39
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spelling	oai:doaj.org-article:f087fa0e992a403aad99c93c50bc6d392021-11-25T06:02:46ZFatty acid profile and unigene-derived simple sequence repeat markers in tung tree (Vernicia fordii).1932-620310.1371/journal.pone.0105298https://doaj.org/article/f087fa0e992a403aad99c93c50bc6d392014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25167054/?tool=EBIhttps://doaj.org/toc/1932-6203Tung tree (Vernicia fordii) provides the sole source of tung oil widely used in industry. Lack of fatty acid composition and molecular markers hinders biochemical, genetic and breeding research. The objectives of this study were to determine fatty acid profiles and develop unigene-derived simple sequence repeat (SSR) markers in tung tree. Fatty acid profiles of 41 accessions showed that the ratio of α-eleostearic acid was increasing continuously with a parallel trend to the amount of tung oil accumulation while the ratios of other fatty acids were decreasing in different stages of the seeds and that α-eleostearic acid (18∶3) consisted of 77% of the total fatty acids in tung oil. Transcriptome sequencing identified 81,805 unigenes from tung cDNA library constructed using seed mRNA and discovered 6,366 SSRs in 5,404 unigenes. The di- and tri-nucleotide microsatellites accounted for 92% of the SSRs with AG/CT and AAG/CTT being the most abundant SSR motifs. Fifteen polymorphic genic-SSR markers were developed from 98 unigene loci tested in 41 cultivated tung accessions by agarose gel and capillary electrophoresis. Genbank database search identified 10 of them putatively coding for functional proteins. Quantitative PCR demonstrated that all 15 polymorphic SSR-associated unigenes were expressed in tung seeds and some of them were highly correlated with oil composition in the seeds. Dendrogram revealed that most of the 41 accessions were clustered according to the geographic region. These new polymorphic genic-SSR markers will facilitate future studies on genetic diversity, molecular fingerprinting, comparative genomics and genetic mapping in tung tree. The lipid profiles in the seeds of 41 tung accessions will be valuable for biochemical and breeding studies.Lin ZhangBaoguang JiaXiaofeng TanChandra S ThamminaHongxu LongMin LiuShanna WenXianliang SongHeping CaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105298 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lin Zhang Baoguang Jia Xiaofeng Tan Chandra S Thammina Hongxu Long Min Liu Shanna Wen Xianliang Song Heping Cao Fatty acid profile and unigene-derived simple sequence repeat markers in tung tree (Vernicia fordii).
description	Tung tree (Vernicia fordii) provides the sole source of tung oil widely used in industry. Lack of fatty acid composition and molecular markers hinders biochemical, genetic and breeding research. The objectives of this study were to determine fatty acid profiles and develop unigene-derived simple sequence repeat (SSR) markers in tung tree. Fatty acid profiles of 41 accessions showed that the ratio of α-eleostearic acid was increasing continuously with a parallel trend to the amount of tung oil accumulation while the ratios of other fatty acids were decreasing in different stages of the seeds and that α-eleostearic acid (18∶3) consisted of 77% of the total fatty acids in tung oil. Transcriptome sequencing identified 81,805 unigenes from tung cDNA library constructed using seed mRNA and discovered 6,366 SSRs in 5,404 unigenes. The di- and tri-nucleotide microsatellites accounted for 92% of the SSRs with AG/CT and AAG/CTT being the most abundant SSR motifs. Fifteen polymorphic genic-SSR markers were developed from 98 unigene loci tested in 41 cultivated tung accessions by agarose gel and capillary electrophoresis. Genbank database search identified 10 of them putatively coding for functional proteins. Quantitative PCR demonstrated that all 15 polymorphic SSR-associated unigenes were expressed in tung seeds and some of them were highly correlated with oil composition in the seeds. Dendrogram revealed that most of the 41 accessions were clustered according to the geographic region. These new polymorphic genic-SSR markers will facilitate future studies on genetic diversity, molecular fingerprinting, comparative genomics and genetic mapping in tung tree. The lipid profiles in the seeds of 41 tung accessions will be valuable for biochemical and breeding studies.
format	article
author	Lin Zhang Baoguang Jia Xiaofeng Tan Chandra S Thammina Hongxu Long Min Liu Shanna Wen Xianliang Song Heping Cao
author_facet	Lin Zhang Baoguang Jia Xiaofeng Tan Chandra S Thammina Hongxu Long Min Liu Shanna Wen Xianliang Song Heping Cao
author_sort	Lin Zhang
title	Fatty acid profile and unigene-derived simple sequence repeat markers in tung tree (Vernicia fordii).
title_short	Fatty acid profile and unigene-derived simple sequence repeat markers in tung tree (Vernicia fordii).
title_full	Fatty acid profile and unigene-derived simple sequence repeat markers in tung tree (Vernicia fordii).
title_fullStr	Fatty acid profile and unigene-derived simple sequence repeat markers in tung tree (Vernicia fordii).
title_full_unstemmed	Fatty acid profile and unigene-derived simple sequence repeat markers in tung tree (Vernicia fordii).
title_sort	fatty acid profile and unigene-derived simple sequence repeat markers in tung tree (vernicia fordii).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f087fa0e992a403aad99c93c50bc6d39
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Fatty acid profile and unigene-derived simple sequence repeat markers in tung tree (Vernicia fordii).

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