Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing

Using CRISPR to write specific genetic sequences can sometimes be difficult due to the preference of mammalian cells to repair breaks using NHEJ. Here the authors form nanoparticles to localize the template sequence to the nuclease, shifting repair in favor of HDR.

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Auteurs principaux:	Jared Carlson-Stevermer, Amr A. Abdeen, Lucille Kohlenberg, Madelyn Goedland, Kaivalya Molugu, Meng Lou, Krishanu Saha
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2017
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/f08b65b2ded04098a2d3fda73679eeab
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spelling	oai:doaj.org-article:f08b65b2ded04098a2d3fda73679eeab2021-12-02T14:42:05ZAssembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing10.1038/s41467-017-01875-92041-1723https://doaj.org/article/f08b65b2ded04098a2d3fda73679eeab2017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01875-9https://doaj.org/toc/2041-1723Using CRISPR to write specific genetic sequences can sometimes be difficult due to the preference of mammalian cells to repair breaks using NHEJ. Here the authors form nanoparticles to localize the template sequence to the nuclease, shifting repair in favor of HDR.Jared Carlson-StevermerAmr A. AbdeenLucille KohlenbergMadelyn GoedlandKaivalya MoluguMeng LouKrishanu SahaNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jared Carlson-Stevermer Amr A. Abdeen Lucille Kohlenberg Madelyn Goedland Kaivalya Molugu Meng Lou Krishanu Saha Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing
description	Using CRISPR to write specific genetic sequences can sometimes be difficult due to the preference of mammalian cells to repair breaks using NHEJ. Here the authors form nanoparticles to localize the template sequence to the nuclease, shifting repair in favor of HDR.
format	article
author	Jared Carlson-Stevermer Amr A. Abdeen Lucille Kohlenberg Madelyn Goedland Kaivalya Molugu Meng Lou Krishanu Saha
author_facet	Jared Carlson-Stevermer Amr A. Abdeen Lucille Kohlenberg Madelyn Goedland Kaivalya Molugu Meng Lou Krishanu Saha
author_sort	Jared Carlson-Stevermer
title	Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing
title_short	Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing
title_full	Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing
title_fullStr	Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing
title_full_unstemmed	Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing
title_sort	assembly of crispr ribonucleoproteins with biotinylated oligonucleotides via an rna aptamer for precise gene editing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f08b65b2ded04098a2d3fda73679eeab
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Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing

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