microRNA-352 regulates collateral vessel growth induced by elevated fluid shear stress in the rat hind limb

Abstract Although collateral vessel growth is distinctly enhanced by elevated fluid shear stress (FSS), the underlying regulatory mechanism of this process remains incompletely understood. Recent studies have shown that microRNAs (miRNAs) play a pivotal role in vascular development, homeostasis and...

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Autores principales:	Yinglu Guan, Baizhen Cai, Xiaoqiong Wu, Song Peng, Liaoying Gan, Da Huang, Guangmin Liu, Liping Dong, Lin Xiao, Junwen Liu, Bin Zhang, Wei-Jun Cai, Jutta Schaper, Wolfgang Schaper
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f08d4a151d2240cdae1f4e79f5cb58a1
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spelling	oai:doaj.org-article:f08d4a151d2240cdae1f4e79f5cb58a12021-12-02T12:30:37ZmicroRNA-352 regulates collateral vessel growth induced by elevated fluid shear stress in the rat hind limb10.1038/s41598-017-06910-92045-2322https://doaj.org/article/f08d4a151d2240cdae1f4e79f5cb58a12017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06910-9https://doaj.org/toc/2045-2322Abstract Although collateral vessel growth is distinctly enhanced by elevated fluid shear stress (FSS), the underlying regulatory mechanism of this process remains incompletely understood. Recent studies have shown that microRNAs (miRNAs) play a pivotal role in vascular development, homeostasis and a variety of diseases. Therefore, this study was designed to identify miRNAs involved in elevated FSS-induced collateral vessel growth in rat hind limbs. A side-to-side arteriovenous (AV) shunt was created between the distal stump of one of the bilaterally occluded femoral arteries and the accompanying vein. The miRNA array profile showed 94 differentially expressed miRNAs in FSS-stressed collaterals including miRNA-352 which was down-regulated. Infusion of antagomir-352 increased the number and proliferation of collateral vessels and promoted collateral flow restoration in a model of rat hind limb ligation. In cell culture studies, the miR-352 inhibitor increased endothelial proliferation, migration and tube formation. In addition, antagomir-352 up-regulated the expression of insulin-like growth factor II receptor (IGF2R), which may play a part in the complex pathway leading to arterial growth. We conclude that enhanced collateral vessel growth is controlled by miRNAs, among which miR-352 is a novel candidate that negatively regulates arteriogenesis, meriting additional studies to unravel the pathways leading to improved collateral circulation.Yinglu GuanBaizhen CaiXiaoqiong WuSong PengLiaoying GanDa HuangGuangmin LiuLiping DongLin XiaoJunwen LiuBin ZhangWei-Jun CaiJutta SchaperWolfgang SchaperNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yinglu Guan Baizhen Cai Xiaoqiong Wu Song Peng Liaoying Gan Da Huang Guangmin Liu Liping Dong Lin Xiao Junwen Liu Bin Zhang Wei-Jun Cai Jutta Schaper Wolfgang Schaper microRNA-352 regulates collateral vessel growth induced by elevated fluid shear stress in the rat hind limb
description	Abstract Although collateral vessel growth is distinctly enhanced by elevated fluid shear stress (FSS), the underlying regulatory mechanism of this process remains incompletely understood. Recent studies have shown that microRNAs (miRNAs) play a pivotal role in vascular development, homeostasis and a variety of diseases. Therefore, this study was designed to identify miRNAs involved in elevated FSS-induced collateral vessel growth in rat hind limbs. A side-to-side arteriovenous (AV) shunt was created between the distal stump of one of the bilaterally occluded femoral arteries and the accompanying vein. The miRNA array profile showed 94 differentially expressed miRNAs in FSS-stressed collaterals including miRNA-352 which was down-regulated. Infusion of antagomir-352 increased the number and proliferation of collateral vessels and promoted collateral flow restoration in a model of rat hind limb ligation. In cell culture studies, the miR-352 inhibitor increased endothelial proliferation, migration and tube formation. In addition, antagomir-352 up-regulated the expression of insulin-like growth factor II receptor (IGF2R), which may play a part in the complex pathway leading to arterial growth. We conclude that enhanced collateral vessel growth is controlled by miRNAs, among which miR-352 is a novel candidate that negatively regulates arteriogenesis, meriting additional studies to unravel the pathways leading to improved collateral circulation.
format	article
author	Yinglu Guan Baizhen Cai Xiaoqiong Wu Song Peng Liaoying Gan Da Huang Guangmin Liu Liping Dong Lin Xiao Junwen Liu Bin Zhang Wei-Jun Cai Jutta Schaper Wolfgang Schaper
author_facet	Yinglu Guan Baizhen Cai Xiaoqiong Wu Song Peng Liaoying Gan Da Huang Guangmin Liu Liping Dong Lin Xiao Junwen Liu Bin Zhang Wei-Jun Cai Jutta Schaper Wolfgang Schaper
author_sort	Yinglu Guan
title	microRNA-352 regulates collateral vessel growth induced by elevated fluid shear stress in the rat hind limb
title_short	microRNA-352 regulates collateral vessel growth induced by elevated fluid shear stress in the rat hind limb
title_full	microRNA-352 regulates collateral vessel growth induced by elevated fluid shear stress in the rat hind limb
title_fullStr	microRNA-352 regulates collateral vessel growth induced by elevated fluid shear stress in the rat hind limb
title_full_unstemmed	microRNA-352 regulates collateral vessel growth induced by elevated fluid shear stress in the rat hind limb
title_sort	microrna-352 regulates collateral vessel growth induced by elevated fluid shear stress in the rat hind limb
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f08d4a151d2240cdae1f4e79f5cb58a1
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microRNA-352 regulates collateral vessel growth induced by elevated fluid shear stress in the rat hind limb

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