In vitro model of vascularized bone: synergizing vascular development and osteogenesis.

Tissue engineering provides unique opportunities for regenerating diseased or damaged tissues using cells obtained from tissue biopsies. Tissue engineered grafts can also be used as high fidelity models to probe cellular and molecular interactions underlying developmental processes. In this study, w...

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Autores principales:	Cristina Correia, Warren L Grayson, Miri Park, Daphne Hutton, Bin Zhou, X Edward Guo, Laura Niklason, Rui A Sousa, Rui L Reis, Gordana Vunjak-Novakovic
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f090df39d2cf4e2aa0bf0f97f6a5ac63
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spelling	oai:doaj.org-article:f090df39d2cf4e2aa0bf0f97f6a5ac632021-11-18T07:33:07ZIn vitro model of vascularized bone: synergizing vascular development and osteogenesis.1932-620310.1371/journal.pone.0028352https://doaj.org/article/f090df39d2cf4e2aa0bf0f97f6a5ac632011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22164277/?tool=EBIhttps://doaj.org/toc/1932-6203Tissue engineering provides unique opportunities for regenerating diseased or damaged tissues using cells obtained from tissue biopsies. Tissue engineered grafts can also be used as high fidelity models to probe cellular and molecular interactions underlying developmental processes. In this study, we co-cultured human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (MSCs) under various environmental conditions to elicit synergistic interactions leading to the colocalized development of capillary-like and bone-like tissues. Cells were encapsulated at the 1:1 ratio in fibrin gel to screen compositions of endothelial growth medium (EGM) and osteogenic medium (OM). It was determined that, to form both tissues, co-cultures should first be supplied with EGM followed by a 1:1 cocktail of the two media types containing bone morphogenetic protein-2. Subsequent studies of HUVECs and MSCs cultured in decellularized, trabecular bone scaffolds for 6 weeks assessed the effects on tissue construct of both temporal variations in growth-factor availability and addition of fresh cells. The resulting grafts were implanted subcutaneously into nude mice to determine the phenotype stability and functionality of engineered vessels. Two important findings resulted from these studies: (i) vascular development needs to be induced prior to osteogenesis, and (ii) the addition of additional hMSCs at the osteogenic induction stage improves both tissue outcomes, as shown by increased bone volume fraction, osteoid deposition, close proximity of bone proteins to vascular networks, and anastomosis of vascular networks with the host vasculature. Interestingly, these observations compare well with what has been described for native development. We propose that our cultivation system can mimic various aspects of endothelial cell-osteogenic precursor interactions in vivo, and could find utility as a model for studies of heterotypic cellular interactions that couple blood vessel formation with osteogenesis.Cristina CorreiaWarren L GraysonMiri ParkDaphne HuttonBin ZhouX Edward GuoLaura NiklasonRui A SousaRui L ReisGordana Vunjak-NovakovicPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28352 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristina Correia Warren L Grayson Miri Park Daphne Hutton Bin Zhou X Edward Guo Laura Niklason Rui A Sousa Rui L Reis Gordana Vunjak-Novakovic In vitro model of vascularized bone: synergizing vascular development and osteogenesis.
description	Tissue engineering provides unique opportunities for regenerating diseased or damaged tissues using cells obtained from tissue biopsies. Tissue engineered grafts can also be used as high fidelity models to probe cellular and molecular interactions underlying developmental processes. In this study, we co-cultured human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (MSCs) under various environmental conditions to elicit synergistic interactions leading to the colocalized development of capillary-like and bone-like tissues. Cells were encapsulated at the 1:1 ratio in fibrin gel to screen compositions of endothelial growth medium (EGM) and osteogenic medium (OM). It was determined that, to form both tissues, co-cultures should first be supplied with EGM followed by a 1:1 cocktail of the two media types containing bone morphogenetic protein-2. Subsequent studies of HUVECs and MSCs cultured in decellularized, trabecular bone scaffolds for 6 weeks assessed the effects on tissue construct of both temporal variations in growth-factor availability and addition of fresh cells. The resulting grafts were implanted subcutaneously into nude mice to determine the phenotype stability and functionality of engineered vessels. Two important findings resulted from these studies: (i) vascular development needs to be induced prior to osteogenesis, and (ii) the addition of additional hMSCs at the osteogenic induction stage improves both tissue outcomes, as shown by increased bone volume fraction, osteoid deposition, close proximity of bone proteins to vascular networks, and anastomosis of vascular networks with the host vasculature. Interestingly, these observations compare well with what has been described for native development. We propose that our cultivation system can mimic various aspects of endothelial cell-osteogenic precursor interactions in vivo, and could find utility as a model for studies of heterotypic cellular interactions that couple blood vessel formation with osteogenesis.
format	article
author	Cristina Correia Warren L Grayson Miri Park Daphne Hutton Bin Zhou X Edward Guo Laura Niklason Rui A Sousa Rui L Reis Gordana Vunjak-Novakovic
author_facet	Cristina Correia Warren L Grayson Miri Park Daphne Hutton Bin Zhou X Edward Guo Laura Niklason Rui A Sousa Rui L Reis Gordana Vunjak-Novakovic
author_sort	Cristina Correia
title	In vitro model of vascularized bone: synergizing vascular development and osteogenesis.
title_short	In vitro model of vascularized bone: synergizing vascular development and osteogenesis.
title_full	In vitro model of vascularized bone: synergizing vascular development and osteogenesis.
title_fullStr	In vitro model of vascularized bone: synergizing vascular development and osteogenesis.
title_full_unstemmed	In vitro model of vascularized bone: synergizing vascular development and osteogenesis.
title_sort	in vitro model of vascularized bone: synergizing vascular development and osteogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f090df39d2cf4e2aa0bf0f97f6a5ac63
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In vitro model of vascularized bone: synergizing vascular development and osteogenesis.

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