Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS)

Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS)

Abstract Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner...

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Bibliographic Details
Main Authors: Janine T. Bossé, Yanwen Li, Leon G. Leanse, Liqing Zhou, Roy R. Chaudhuri, Sarah E. Peters, Jinhong Wang, Gareth A. Maglennon, Matthew T. G. Holden, Duncan J. Maskell, Alexander W. Tucker, Brendan W. Wren, Andrew N. Rycroft, Paul R. Langford, on behalf of the BRaDP1T consortium
Format: article
Language:EN
Published: BMC 2021
Subjects:
Mariner
Transposon
TraDIS
Pasteurellaceae
Actinobacillus pleuropneumoniae
Pasteurella multocida
Veterinary medicine
SF600-1100
Public aspects of medicine
RA1-1270
Online Access:https://doaj.org/article/f094b55991ac4fa1b5e1c9c4b524d061
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