Enhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan

Naim Kittana,1 Mohyeddin Assali,2 Hanood Abu-Rass,1 Susanne Lutz,3,4 Rama Hindawi,2 Lina Ghannam,2 Marah Zakarneh,2 Ahmad Mousa1 1Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine; 2Department of Pharmacy, Faculty of Medicine...

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Autores principales:	Kittana N, Assali M, Abu-Rass H, Lutz S, Hindawi R, Ghannam L, Zakarneh M, Mousa A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Carbon nanotubes chitosan wound healing re-epithelialization fibrosis inflammation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f0a02118361e4071befc183fcfadbdb0
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spelling	oai:doaj.org-article:f0a02118361e4071befc183fcfadbdb02021-12-02T07:58:45ZEnhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan1178-2013https://doaj.org/article/f0a02118361e4071befc183fcfadbdb02018-11-01T00:00:00Zhttps://www.dovepress.com/enhancement-of-wound-healing-by-single-wallmulti-wall-carbon-nanotubes-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Naim Kittana,1 Mohyeddin Assali,2 Hanood Abu-Rass,1 Susanne Lutz,3,4 Rama Hindawi,2 Lina Ghannam,2 Marah Zakarneh,2 Ahmad Mousa1 1Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine; 2Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine; 3Institute of Pharmacology and Toxicology, University Medical Center, Göttingen, Germany; 4German Center for Cardiovascular Research e.V., Partner Site, Göttingen, Germany Background: Impaired wound healing is commonly associated with many health problems, including diabetes, bedsores and extensive burns. In such cases, healing often takes a long time, which subjects patients to various complications. This study aims to investigate whether single-wall or multi-wall carbon nanotubes complexed with chitosan hydrogel can improve wound healing. Materials and methods: Initially, the effects of the complexes on the viability and functionality of fibroblasts were investigated in engineered connective tissues. Then, their activity on wound healing was investigated in a mouse model with induced full-thickness wounds, in which the wounds were treated daily with these complexes. Finally, the effect of the complexes on collagen deposition by fibroblasts was investigated in vitro. Results: The engineered connective tissue studies showed that fibroblasts were viable in the presence of the complexes and were still able to effectively organize and contract the extracellular matrix. In vivo data showed that both types of complexes improved the re-epithelialization of the healing wounds; however, they also increased the percentage of wounds with higher fibrosis. In particular, the chitosan-multi-wall carbon nanotube complex significantly enhanced the extensiveness of this fibrosis, which is in line with in vitro data showing a concentration-dependent enhancement of collage deposition by these complexes. These observations were associated with an increase in inflammatory signs in the wound bed. Conclusion: Single-wall and multi-wall carbon nanotubes complexed with chitosan improved the re-epithelialization of wounds, but an increase in fibrosis was detected. Keywords: carbon nanotubes, chitosan, wound healing, re-epithelialization, fibrosis, inflammationKittana NAssali MAbu-Rass HLutz SHindawi RGhannam LZakarneh MMousa ADove Medical PressarticleCarbon nanotubeschitosanwound healingre-epithelializationfibrosisinflammationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 7195-7206 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Carbon nanotubes chitosan wound healing re-epithelialization fibrosis inflammation Medicine (General) R5-920
spellingShingle	Carbon nanotubes chitosan wound healing re-epithelialization fibrosis inflammation Medicine (General) R5-920 Kittana N Assali M Abu-Rass H Lutz S Hindawi R Ghannam L Zakarneh M Mousa A Enhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan
description	Naim Kittana,1 Mohyeddin Assali,2 Hanood Abu-Rass,1 Susanne Lutz,3,4 Rama Hindawi,2 Lina Ghannam,2 Marah Zakarneh,2 Ahmad Mousa1 1Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine; 2Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine; 3Institute of Pharmacology and Toxicology, University Medical Center, Göttingen, Germany; 4German Center for Cardiovascular Research e.V., Partner Site, Göttingen, Germany Background: Impaired wound healing is commonly associated with many health problems, including diabetes, bedsores and extensive burns. In such cases, healing often takes a long time, which subjects patients to various complications. This study aims to investigate whether single-wall or multi-wall carbon nanotubes complexed with chitosan hydrogel can improve wound healing. Materials and methods: Initially, the effects of the complexes on the viability and functionality of fibroblasts were investigated in engineered connective tissues. Then, their activity on wound healing was investigated in a mouse model with induced full-thickness wounds, in which the wounds were treated daily with these complexes. Finally, the effect of the complexes on collagen deposition by fibroblasts was investigated in vitro. Results: The engineered connective tissue studies showed that fibroblasts were viable in the presence of the complexes and were still able to effectively organize and contract the extracellular matrix. In vivo data showed that both types of complexes improved the re-epithelialization of the healing wounds; however, they also increased the percentage of wounds with higher fibrosis. In particular, the chitosan-multi-wall carbon nanotube complex significantly enhanced the extensiveness of this fibrosis, which is in line with in vitro data showing a concentration-dependent enhancement of collage deposition by these complexes. These observations were associated with an increase in inflammatory signs in the wound bed. Conclusion: Single-wall and multi-wall carbon nanotubes complexed with chitosan improved the re-epithelialization of wounds, but an increase in fibrosis was detected. Keywords: carbon nanotubes, chitosan, wound healing, re-epithelialization, fibrosis, inflammation
format	article
author	Kittana N Assali M Abu-Rass H Lutz S Hindawi R Ghannam L Zakarneh M Mousa A
author_facet	Kittana N Assali M Abu-Rass H Lutz S Hindawi R Ghannam L Zakarneh M Mousa A
author_sort	Kittana N
title	Enhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan
title_short	Enhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan
title_full	Enhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan
title_fullStr	Enhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan
title_full_unstemmed	Enhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan
title_sort	enhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/f0a02118361e4071befc183fcfadbdb0
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Enhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan

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