Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication.

Recent advances in microRNA target identification have greatly increased the number of putative targets of viral microRNAs. However, it is still unclear whether all targets identified are biologically relevant. Here, we use a combined approach of RISC immunoprecipitation and focused siRNA screening...

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Autores principales:	Jon Pavelin, Natalie Reynolds, Stephen Chiweshe, Guanming Wu, Rebecca Tiribassi, Finn Grey
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f0a0af131dc04466b612cf1575887d35
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spelling	oai:doaj.org-article:f0a0af131dc04466b612cf1575887d352021-11-18T06:07:11ZSystematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication.1553-73661553-737410.1371/journal.ppat.1003820https://doaj.org/article/f0a0af131dc04466b612cf1575887d352013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24385903/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Recent advances in microRNA target identification have greatly increased the number of putative targets of viral microRNAs. However, it is still unclear whether all targets identified are biologically relevant. Here, we use a combined approach of RISC immunoprecipitation and focused siRNA screening to identify targets of HCMV encoded human cytomegalovirus that play an important role in the biology of the virus. Using both a laboratory and clinical strain of human cytomegalovirus, we identify over 200 putative targets of human cytomegalovirus microRNAs following infection of fibroblast cells. By comparing RISC-IP profiles of miRNA knockout viruses, we have resolved specific interactions between human cytomegalovirus miRNAs and the top candidate target transcripts and validated regulation by western blot analysis and luciferase assay. Crucially we demonstrate that miRNA target genes play important roles in the biology of human cytomegalovirus as siRNA knockdown results in marked effects on virus replication. The most striking phenotype followed knockdown of the top target ATP6V0C, which is required for endosomal acidification. siRNA knockdown of ATP6V0C resulted in almost complete loss of infectious virus production, suggesting that an HCMV microRNA targets a crucial cellular factor required for virus replication. This study greatly increases the number of identified targets of human cytomegalovirus microRNAs and demonstrates the effective use of combined miRNA target identification and focused siRNA screening for identifying novel host virus interactions.Jon PavelinNatalie ReynoldsStephen ChiwesheGuanming WuRebecca TiribassiFinn GreyPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 12, p e1003820 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Jon Pavelin Natalie Reynolds Stephen Chiweshe Guanming Wu Rebecca Tiribassi Finn Grey Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication.
description	Recent advances in microRNA target identification have greatly increased the number of putative targets of viral microRNAs. However, it is still unclear whether all targets identified are biologically relevant. Here, we use a combined approach of RISC immunoprecipitation and focused siRNA screening to identify targets of HCMV encoded human cytomegalovirus that play an important role in the biology of the virus. Using both a laboratory and clinical strain of human cytomegalovirus, we identify over 200 putative targets of human cytomegalovirus microRNAs following infection of fibroblast cells. By comparing RISC-IP profiles of miRNA knockout viruses, we have resolved specific interactions between human cytomegalovirus miRNAs and the top candidate target transcripts and validated regulation by western blot analysis and luciferase assay. Crucially we demonstrate that miRNA target genes play important roles in the biology of human cytomegalovirus as siRNA knockdown results in marked effects on virus replication. The most striking phenotype followed knockdown of the top target ATP6V0C, which is required for endosomal acidification. siRNA knockdown of ATP6V0C resulted in almost complete loss of infectious virus production, suggesting that an HCMV microRNA targets a crucial cellular factor required for virus replication. This study greatly increases the number of identified targets of human cytomegalovirus microRNAs and demonstrates the effective use of combined miRNA target identification and focused siRNA screening for identifying novel host virus interactions.
format	article
author	Jon Pavelin Natalie Reynolds Stephen Chiweshe Guanming Wu Rebecca Tiribassi Finn Grey
author_facet	Jon Pavelin Natalie Reynolds Stephen Chiweshe Guanming Wu Rebecca Tiribassi Finn Grey
author_sort	Jon Pavelin
title	Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication.
title_short	Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication.
title_full	Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication.
title_fullStr	Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication.
title_full_unstemmed	Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication.
title_sort	systematic microrna analysis identifies atp6v0c as an essential host factor for human cytomegalovirus replication.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f0a0af131dc04466b612cf1575887d35
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Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication.

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