Nanotopological plate stimulates osteogenic differentiation through TAZ activation

Abstract The topographical environment, which mimics the stem cell niche, provides mechanical cues to regulate the differentiation of mesenchymal stem cells (MSC). Diverse topographical variations have been engineered to investigate cellular responses; however, the types of mechanical parameters tha...

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Autores principales:	Jun-Ha Hwang, Dong-Hyun Lee, Mi Ran Byun, A. Rum Kim, Kyung Min Kim, Jung Il Park, Ho Taek Oh, Eun Sook Hwang, Kyu Back Lee, Jeong-Ho Hong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f0a0b7b85efc4417a4ed3f10658eb992
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spelling	oai:doaj.org-article:f0a0b7b85efc4417a4ed3f10658eb9922021-12-02T15:05:36ZNanotopological plate stimulates osteogenic differentiation through TAZ activation10.1038/s41598-017-03815-52045-2322https://doaj.org/article/f0a0b7b85efc4417a4ed3f10658eb9922017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03815-5https://doaj.org/toc/2045-2322Abstract The topographical environment, which mimics the stem cell niche, provides mechanical cues to regulate the differentiation of mesenchymal stem cells (MSC). Diverse topographical variations have been engineered to investigate cellular responses; however, the types of mechanical parameters that affect cells, and their underlying mechanisms remain largely unknown. In this study, we screened nanotopological pillars with size gradient to activate transcriptional coactivator with PDZ binding motif (TAZ), which stimulates osteogenesis of MSC. We observed that a nanotopological plate, 70 nm in diameter, significantly induces osteogenic differentiation with the activation of TAZ. TAZ activation via the nanotopological plate was mediated by actin polymerization and Rho signaling, as evidenced by the cytosolic localization of TAZ under F-actin or Rho kinase inhibitor. The FAK and MAPK pathways also play a role in TAZ activation by the nanotopological plate because the inhibitor of ERK and JNK blocked nanopattern plate induced osteogenic differentiation. Taken together, these results indicate that nanotopology regulates cell differentiation through TAZ activation.Jun-Ha HwangDong-Hyun LeeMi Ran ByunA. Rum KimKyung Min KimJung Il ParkHo Taek OhEun Sook HwangKyu Back LeeJeong-Ho HongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun-Ha Hwang Dong-Hyun Lee Mi Ran Byun A. Rum Kim Kyung Min Kim Jung Il Park Ho Taek Oh Eun Sook Hwang Kyu Back Lee Jeong-Ho Hong Nanotopological plate stimulates osteogenic differentiation through TAZ activation
description	Abstract The topographical environment, which mimics the stem cell niche, provides mechanical cues to regulate the differentiation of mesenchymal stem cells (MSC). Diverse topographical variations have been engineered to investigate cellular responses; however, the types of mechanical parameters that affect cells, and their underlying mechanisms remain largely unknown. In this study, we screened nanotopological pillars with size gradient to activate transcriptional coactivator with PDZ binding motif (TAZ), which stimulates osteogenesis of MSC. We observed that a nanotopological plate, 70 nm in diameter, significantly induces osteogenic differentiation with the activation of TAZ. TAZ activation via the nanotopological plate was mediated by actin polymerization and Rho signaling, as evidenced by the cytosolic localization of TAZ under F-actin or Rho kinase inhibitor. The FAK and MAPK pathways also play a role in TAZ activation by the nanotopological plate because the inhibitor of ERK and JNK blocked nanopattern plate induced osteogenic differentiation. Taken together, these results indicate that nanotopology regulates cell differentiation through TAZ activation.
format	article
author	Jun-Ha Hwang Dong-Hyun Lee Mi Ran Byun A. Rum Kim Kyung Min Kim Jung Il Park Ho Taek Oh Eun Sook Hwang Kyu Back Lee Jeong-Ho Hong
author_facet	Jun-Ha Hwang Dong-Hyun Lee Mi Ran Byun A. Rum Kim Kyung Min Kim Jung Il Park Ho Taek Oh Eun Sook Hwang Kyu Back Lee Jeong-Ho Hong
author_sort	Jun-Ha Hwang
title	Nanotopological plate stimulates osteogenic differentiation through TAZ activation
title_short	Nanotopological plate stimulates osteogenic differentiation through TAZ activation
title_full	Nanotopological plate stimulates osteogenic differentiation through TAZ activation
title_fullStr	Nanotopological plate stimulates osteogenic differentiation through TAZ activation
title_full_unstemmed	Nanotopological plate stimulates osteogenic differentiation through TAZ activation
title_sort	nanotopological plate stimulates osteogenic differentiation through taz activation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f0a0b7b85efc4417a4ed3f10658eb992
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_version_	1718388803517808640

Nanotopological plate stimulates osteogenic differentiation through TAZ activation

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