Development of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus
African swine fever virus (ASFV) can infect domestic pigs and wild boars and causes huge economic losses in global swine industry. Therefore, early diagnosis of ASFV is important for the control and eradication of African swine fever (ASF). In this study, a SYBR Green-based real-time polymerase chai...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:f0a236763fa243cdb34ea8e05929f5232021-12-01T13:13:35ZDevelopment of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus2297-176910.3389/fvets.2021.753967https://doaj.org/article/f0a236763fa243cdb34ea8e05929f5232021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.753967/fullhttps://doaj.org/toc/2297-1769African swine fever virus (ASFV) can infect domestic pigs and wild boars and causes huge economic losses in global swine industry. Therefore, early diagnosis of ASFV is important for the control and eradication of African swine fever (ASF). In this study, a SYBR Green-based real-time polymerase chain reaction (PCR) assay targeting the viral encoded A137R gene was established for the detection of ASFV infection. For the evaluation of the established real-time PCR, 34 clinical samples were assessed by both the A137R gene-based real-time PCR and OIE-recommended TaqMan PCR. The results showed that 85.29% (29/34) were detected by A137R gene-based real-time PCR, but only 79.41% (27/34) positive using OIE-recommended TaqMan PCR. Moreover, no cross-reaction with other common swine pathogens was found in the A137R gene-based real-time PCR. These results demonstrated that the established real-time PCR assay in this study showed better performance than the OIE-recommended method in detecting ASFV from clinical samples, which could be applied for control and eradication programs of ASF.Dan YinDan YinDan YinRenhao GengRenhao GengRenhao GengHui LvHui LvHui LvChunhui BaoChunhui BaoChunhui BaoHongxia ShaoHongxia ShaoHongxia ShaoJianqiang YeJianqiang YeJianqiang YeKun QianKun QianKun QianAijian QinAijian QinAijian QinFrontiers Media S.A.articleAfrican swine fever virusA137R geneSYBR Greenreal-time PCRdetectionVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021) |
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EN |
| topic |
African swine fever virus A137R gene SYBR Green real-time PCR detection Veterinary medicine SF600-1100 |
| spellingShingle |
African swine fever virus A137R gene SYBR Green real-time PCR detection Veterinary medicine SF600-1100 Dan Yin Dan Yin Dan Yin Renhao Geng Renhao Geng Renhao Geng Hui Lv Hui Lv Hui Lv Chunhui Bao Chunhui Bao Chunhui Bao Hongxia Shao Hongxia Shao Hongxia Shao Jianqiang Ye Jianqiang Ye Jianqiang Ye Kun Qian Kun Qian Kun Qian Aijian Qin Aijian Qin Aijian Qin Development of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus |
| description |
African swine fever virus (ASFV) can infect domestic pigs and wild boars and causes huge economic losses in global swine industry. Therefore, early diagnosis of ASFV is important for the control and eradication of African swine fever (ASF). In this study, a SYBR Green-based real-time polymerase chain reaction (PCR) assay targeting the viral encoded A137R gene was established for the detection of ASFV infection. For the evaluation of the established real-time PCR, 34 clinical samples were assessed by both the A137R gene-based real-time PCR and OIE-recommended TaqMan PCR. The results showed that 85.29% (29/34) were detected by A137R gene-based real-time PCR, but only 79.41% (27/34) positive using OIE-recommended TaqMan PCR. Moreover, no cross-reaction with other common swine pathogens was found in the A137R gene-based real-time PCR. These results demonstrated that the established real-time PCR assay in this study showed better performance than the OIE-recommended method in detecting ASFV from clinical samples, which could be applied for control and eradication programs of ASF. |
| format |
article |
| author |
Dan Yin Dan Yin Dan Yin Renhao Geng Renhao Geng Renhao Geng Hui Lv Hui Lv Hui Lv Chunhui Bao Chunhui Bao Chunhui Bao Hongxia Shao Hongxia Shao Hongxia Shao Jianqiang Ye Jianqiang Ye Jianqiang Ye Kun Qian Kun Qian Kun Qian Aijian Qin Aijian Qin Aijian Qin |
| author_facet |
Dan Yin Dan Yin Dan Yin Renhao Geng Renhao Geng Renhao Geng Hui Lv Hui Lv Hui Lv Chunhui Bao Chunhui Bao Chunhui Bao Hongxia Shao Hongxia Shao Hongxia Shao Jianqiang Ye Jianqiang Ye Jianqiang Ye Kun Qian Kun Qian Kun Qian Aijian Qin Aijian Qin Aijian Qin |
| author_sort |
Dan Yin |
| title |
Development of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus |
| title_short |
Development of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus |
| title_full |
Development of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus |
| title_fullStr |
Development of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus |
| title_full_unstemmed |
Development of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus |
| title_sort |
development of real-time pcr based on a137r gene for the detection of african swine fever virus |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/f0a236763fa243cdb34ea8e05929f523 |
| work_keys_str_mv |
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