<italic toggle="yes">In Vivo</italic> Imaging of Influenza Virus Infection in Immunized Mice

ABSTRACT Immunization is the cornerstone of seasonal influenza control and represents an important component of pandemic preparedness strategies. Using a bioluminescent reporter virus, we demonstrate the application of noninvasive in vivo imaging system (IVIS) technology to evaluate the preclinical...

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Autores principales:	Rita Czakó, Leatrice Vogel, Elaine W. Lamirande, Kevin W. Bock, Ian N. Moore, Ali H. Ellebedy, Rafi Ahmed, Andrew Mehle, Kanta Subbarao
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f0a51428445a4e2bb5f4194103812693
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spelling	oai:doaj.org-article:f0a51428445a4e2bb5f41941038126932021-11-15T15:51:30Z<italic toggle="yes">In Vivo</italic> Imaging of Influenza Virus Infection in Immunized Mice10.1128/mBio.00714-172150-7511https://doaj.org/article/f0a51428445a4e2bb5f41941038126932017-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00714-17https://doaj.org/toc/2150-7511ABSTRACT Immunization is the cornerstone of seasonal influenza control and represents an important component of pandemic preparedness strategies. Using a bioluminescent reporter virus, we demonstrate the application of noninvasive in vivo imaging system (IVIS) technology to evaluate the preclinical efficacy of candidate vaccines and immunotherapy in a mouse model of influenza. Sequential imaging revealed distinct spatiotemporal kinetics of bioluminescence in groups of mice passively or actively immunized by various strategies that accelerated the clearance of the challenge virus at different rates and by distinct mechanisms. Imaging findings were consistent with conclusions derived from virus titers in the lungs and, notably, were more informative than conventional efficacy endpoints in some cases. Our findings demonstrate the reliability of IVIS as a qualitative approach to support preclinical evaluation of candidate medical countermeasures for influenza in mice. IMPORTANCE Influenza A viruses remain a persistent threat to public health. Vaccination and immunotherapy are effective countermeasures for the control of influenza but must contend with antigenic drift and the risk of resistance to antivirals. Traditional preclinical efficacy studies for novel vaccine and pharmaceutical candidates can be time-consuming and expensive and are inherently limited in scope. In vivo imaging approaches offer the potential to noninvasively track virus replication in real time in animal models. In this study, we demonstrate the utility of bioluminescent imaging for tracking influenza virus replication in the lungs of immunized mice and also identify important factors that may influence the accurate interpretation of imaging results. Our findings support the potential of IVIS approaches to enhance traditional preclinical efficacy evaluation of candidate vaccines and human monoclonal antibodies for the prevention and treatment of influenza.Rita CzakóLeatrice VogelElaine W. LamirandeKevin W. BockIan N. MooreAli H. EllebedyRafi AhmedAndrew MehleKanta SubbaraoAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 8, Iss 3 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Rita Czakó Leatrice Vogel Elaine W. Lamirande Kevin W. Bock Ian N. Moore Ali H. Ellebedy Rafi Ahmed Andrew Mehle Kanta Subbarao <italic toggle="yes">In Vivo</italic> Imaging of Influenza Virus Infection in Immunized Mice
description	ABSTRACT Immunization is the cornerstone of seasonal influenza control and represents an important component of pandemic preparedness strategies. Using a bioluminescent reporter virus, we demonstrate the application of noninvasive in vivo imaging system (IVIS) technology to evaluate the preclinical efficacy of candidate vaccines and immunotherapy in a mouse model of influenza. Sequential imaging revealed distinct spatiotemporal kinetics of bioluminescence in groups of mice passively or actively immunized by various strategies that accelerated the clearance of the challenge virus at different rates and by distinct mechanisms. Imaging findings were consistent with conclusions derived from virus titers in the lungs and, notably, were more informative than conventional efficacy endpoints in some cases. Our findings demonstrate the reliability of IVIS as a qualitative approach to support preclinical evaluation of candidate medical countermeasures for influenza in mice. IMPORTANCE Influenza A viruses remain a persistent threat to public health. Vaccination and immunotherapy are effective countermeasures for the control of influenza but must contend with antigenic drift and the risk of resistance to antivirals. Traditional preclinical efficacy studies for novel vaccine and pharmaceutical candidates can be time-consuming and expensive and are inherently limited in scope. In vivo imaging approaches offer the potential to noninvasively track virus replication in real time in animal models. In this study, we demonstrate the utility of bioluminescent imaging for tracking influenza virus replication in the lungs of immunized mice and also identify important factors that may influence the accurate interpretation of imaging results. Our findings support the potential of IVIS approaches to enhance traditional preclinical efficacy evaluation of candidate vaccines and human monoclonal antibodies for the prevention and treatment of influenza.
format	article
author	Rita Czakó Leatrice Vogel Elaine W. Lamirande Kevin W. Bock Ian N. Moore Ali H. Ellebedy Rafi Ahmed Andrew Mehle Kanta Subbarao
author_facet	Rita Czakó Leatrice Vogel Elaine W. Lamirande Kevin W. Bock Ian N. Moore Ali H. Ellebedy Rafi Ahmed Andrew Mehle Kanta Subbarao
author_sort	Rita Czakó
title	<italic toggle="yes">In Vivo</italic> Imaging of Influenza Virus Infection in Immunized Mice
title_short	<italic toggle="yes">In Vivo</italic> Imaging of Influenza Virus Infection in Immunized Mice
title_full	<italic toggle="yes">In Vivo</italic> Imaging of Influenza Virus Infection in Immunized Mice
title_fullStr	<italic toggle="yes">In Vivo</italic> Imaging of Influenza Virus Infection in Immunized Mice
title_full_unstemmed	<italic toggle="yes">In Vivo</italic> Imaging of Influenza Virus Infection in Immunized Mice
title_sort	<italic toggle="yes">in vivo</italic> imaging of influenza virus infection in immunized mice
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/f0a51428445a4e2bb5f4194103812693
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<italic toggle="yes">In Vivo</italic> Imaging of Influenza Virus Infection in Immunized Mice

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