Physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection

Abstract Electrostatic samplers have been increasingly studied for sampling of viral and bacterial aerosols, and bioaerosol samplers are required to provide concentrated liquid samples with high physical collection and biological recovery, which would be critical for rapid detection. Here, the effec...

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Autores principales:	Seongkyeol Hong, Myeong-Woo Kim, Jaesung Jang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f0aeff05789846b39edf47af0dcb0739
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spelling	oai:doaj.org-article:f0aeff05789846b39edf47af0dcb07392021-12-02T18:31:29ZPhysical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection10.1038/s41598-021-94033-72045-2322https://doaj.org/article/f0aeff05789846b39edf47af0dcb07392021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94033-7https://doaj.org/toc/2045-2322Abstract Electrostatic samplers have been increasingly studied for sampling of viral and bacterial aerosols, and bioaerosol samplers are required to provide concentrated liquid samples with high physical collection and biological recovery, which would be critical for rapid detection. Here, the effects of sampling media and protocols on the physical collection and biological recovery of two airborne bacteria (Pseudomonas fluorescens and Micrococcus luteus) under electrostatic field were investigated using a personal electrostatic particle concentrator (EPC). Deionized (DI) water with/without sodium dodecyl sulfate (SDS) and phosphate buffered saline were tested as sampling media. A polystyrene container was mounted onto the collection electrode of the EPC for stable storage and vortexing after capture. Many bacterial cells were found to be deposited on the bottom surface of the container submerged in the media via electrophoresis, and among the tested sampling protocols, wet sampling with a container and subsequent vortexing offered the most bacteria in the collection suspension. Experiments with several sampling media showed that 0.001–0.01% SDS-DI water demonstrated the highest recovery rate in the EPC. These findings would be valuable in the field of sampling and subsequent rapid detection of bioaerosols.Seongkyeol HongMyeong-Woo KimJaesung JangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seongkyeol Hong Myeong-Woo Kim Jaesung Jang Physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection
description	Abstract Electrostatic samplers have been increasingly studied for sampling of viral and bacterial aerosols, and bioaerosol samplers are required to provide concentrated liquid samples with high physical collection and biological recovery, which would be critical for rapid detection. Here, the effects of sampling media and protocols on the physical collection and biological recovery of two airborne bacteria (Pseudomonas fluorescens and Micrococcus luteus) under electrostatic field were investigated using a personal electrostatic particle concentrator (EPC). Deionized (DI) water with/without sodium dodecyl sulfate (SDS) and phosphate buffered saline were tested as sampling media. A polystyrene container was mounted onto the collection electrode of the EPC for stable storage and vortexing after capture. Many bacterial cells were found to be deposited on the bottom surface of the container submerged in the media via electrophoresis, and among the tested sampling protocols, wet sampling with a container and subsequent vortexing offered the most bacteria in the collection suspension. Experiments with several sampling media showed that 0.001–0.01% SDS-DI water demonstrated the highest recovery rate in the EPC. These findings would be valuable in the field of sampling and subsequent rapid detection of bioaerosols.
format	article
author	Seongkyeol Hong Myeong-Woo Kim Jaesung Jang
author_facet	Seongkyeol Hong Myeong-Woo Kim Jaesung Jang
author_sort	Seongkyeol Hong
title	Physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection
title_short	Physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection
title_full	Physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection
title_fullStr	Physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection
title_full_unstemmed	Physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection
title_sort	physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f0aeff05789846b39edf47af0dcb0739
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_version_	1718378015388336128

Physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection

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