miRNA-mRNA integrated analysis reveals roles for miRNAs in primary breast tumors.
<h4>Introduction</h4>Few studies have performed expression profiling of both miRNA and mRNA from the same primary breast carcinomas. In this study we present and analyze data derived from expression profiling of 799 miRNAs in 101 primary human breast tumors, along with genome-wide mRNA p...
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Public Library of Science (PLoS)
2011
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oai:doaj.org-article:f0b60a48f8f24d3fa8a638ab6d8cf7732021-11-18T06:58:30ZmiRNA-mRNA integrated analysis reveals roles for miRNAs in primary breast tumors.1932-620310.1371/journal.pone.0016915https://doaj.org/article/f0b60a48f8f24d3fa8a638ab6d8cf7732011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21364938/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Introduction</h4>Few studies have performed expression profiling of both miRNA and mRNA from the same primary breast carcinomas. In this study we present and analyze data derived from expression profiling of 799 miRNAs in 101 primary human breast tumors, along with genome-wide mRNA profiles and extensive clinical information.<h4>Methods</h4>We investigate the relationship between these molecular components, in terms of their correlation with each other and with clinical characteristics. We use a systems biology approach to examine the correlative relationship between miRNA and mRNAs using statistical enrichment methods.<h4>Results</h4>We identify statistical significant differential expression of miRNAs between molecular intrinsic subtypes, and between samples with different levels of proliferation. Specifically, we point to miRNAs significantly associated with TP53 and ER status. We also show that several cellular processes, such as proliferation, cell adhesion and immune response, are strongly associated with certain miRNAs. We validate the role of miRNAs in regulating proliferation using high-throughput lysate-microarrays on cell lines and point to potential drivers of this process.<h4>Conclusion</h4>This study provides a comprehensive dataset as well as methods and system-level results that jointly form a basis for further work on understanding the role of miRNA in primary breast cancer.Espen EnerlyIsrael SteinfeldKristine KleiviSuvi-Katri LeivonenMiriam R AureHege G RussnesJo Anders RønnebergHilde JohnsenRoy NavonEinar RødlandRami MäkeläBjørn NaumeMerja PeräläOlli KallioniemiVessela N KristensenZohar YakhiniAnne-Lise Børresen-DalePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e16915 (2011) |
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Medicine R Science Q Espen Enerly Israel Steinfeld Kristine Kleivi Suvi-Katri Leivonen Miriam R Aure Hege G Russnes Jo Anders Rønneberg Hilde Johnsen Roy Navon Einar Rødland Rami Mäkelä Bjørn Naume Merja Perälä Olli Kallioniemi Vessela N Kristensen Zohar Yakhini Anne-Lise Børresen-Dale miRNA-mRNA integrated analysis reveals roles for miRNAs in primary breast tumors. |
| description |
<h4>Introduction</h4>Few studies have performed expression profiling of both miRNA and mRNA from the same primary breast carcinomas. In this study we present and analyze data derived from expression profiling of 799 miRNAs in 101 primary human breast tumors, along with genome-wide mRNA profiles and extensive clinical information.<h4>Methods</h4>We investigate the relationship between these molecular components, in terms of their correlation with each other and with clinical characteristics. We use a systems biology approach to examine the correlative relationship between miRNA and mRNAs using statistical enrichment methods.<h4>Results</h4>We identify statistical significant differential expression of miRNAs between molecular intrinsic subtypes, and between samples with different levels of proliferation. Specifically, we point to miRNAs significantly associated with TP53 and ER status. We also show that several cellular processes, such as proliferation, cell adhesion and immune response, are strongly associated with certain miRNAs. We validate the role of miRNAs in regulating proliferation using high-throughput lysate-microarrays on cell lines and point to potential drivers of this process.<h4>Conclusion</h4>This study provides a comprehensive dataset as well as methods and system-level results that jointly form a basis for further work on understanding the role of miRNA in primary breast cancer. |
| format |
article |
| author |
Espen Enerly Israel Steinfeld Kristine Kleivi Suvi-Katri Leivonen Miriam R Aure Hege G Russnes Jo Anders Rønneberg Hilde Johnsen Roy Navon Einar Rødland Rami Mäkelä Bjørn Naume Merja Perälä Olli Kallioniemi Vessela N Kristensen Zohar Yakhini Anne-Lise Børresen-Dale |
| author_facet |
Espen Enerly Israel Steinfeld Kristine Kleivi Suvi-Katri Leivonen Miriam R Aure Hege G Russnes Jo Anders Rønneberg Hilde Johnsen Roy Navon Einar Rødland Rami Mäkelä Bjørn Naume Merja Perälä Olli Kallioniemi Vessela N Kristensen Zohar Yakhini Anne-Lise Børresen-Dale |
| author_sort |
Espen Enerly |
| title |
miRNA-mRNA integrated analysis reveals roles for miRNAs in primary breast tumors. |
| title_short |
miRNA-mRNA integrated analysis reveals roles for miRNAs in primary breast tumors. |
| title_full |
miRNA-mRNA integrated analysis reveals roles for miRNAs in primary breast tumors. |
| title_fullStr |
miRNA-mRNA integrated analysis reveals roles for miRNAs in primary breast tumors. |
| title_full_unstemmed |
miRNA-mRNA integrated analysis reveals roles for miRNAs in primary breast tumors. |
| title_sort |
mirna-mrna integrated analysis reveals roles for mirnas in primary breast tumors. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/f0b60a48f8f24d3fa8a638ab6d8cf773 |
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