DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS
Present study has shown that a total cytokine pool produced by human blood cells in presence of proteins from the γ-globulin fraction, or their complexes with copper and zinc, as well as with pure copper and zinc cations, contains up to 148.0±14.9 pg interleukin-1β (IL-1β) per mL. The γ-globulins mo...
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oai:doaj.org-article:f0bb304fb0584dddb2a14ede7e62e3d02021-11-18T08:03:41ZDETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS1563-06252313-741X10.15789/1563-0625-2010-6-497-502https://doaj.org/article/f0bb304fb0584dddb2a14ede7e62e3d02014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/437https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XPresent study has shown that a total cytokine pool produced by human blood cells in presence of proteins from the γ-globulin fraction, or their complexes with copper and zinc, as well as with pure copper and zinc cations, contains up to 148.0±14.9 pg interleukin-1β (IL-1β) per mL. The γ-globulins modified by copper or zinc binding induce, resp., 2.0 and 1.4-fold less IL-1β than intact proteins (significant by p = 0.1). Meanwhile, the copper-γ-globulin proved to be 2.7-fold more active (р < 0.01) IL-1β inducer, as a zinc-protein complex. Pure zinc ions did not induce IL-1β production, whereas induction rates with pure copper ions are 1.6-fold higher (p < 0.05) than with γ-globulin, and 1.45-fold more efficient that PHA stimulation (p < 0.1). A potential participation of copper and zinc cations bound to antibodies from the microenvironment and, thus, transforming Fc regions of antibody molecules, is discussed with regard of regulation of IL-1β production by human blood cells induced by activation of their Fc receptors.S. B. CheknevI. E. EfremovaL. S. PiskovskayaE. N. YushkovetsA. A. BabajanzSPb RAACIarticleγ-globulinmetal complexesil-1βinductionproductionImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 12, Iss 6, Pp 497-502 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
RU |
| topic |
γ-globulin metal complexes il-1β induction production Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
γ-globulin metal complexes il-1β induction production Immunologic diseases. Allergy RC581-607 S. B. Cheknev I. E. Efremova L. S. Piskovskaya E. N. Yushkovets A. A. Babajanz DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS |
| description |
Present study has shown that a total cytokine pool produced by human blood cells in presence of proteins from the γ-globulin fraction, or their complexes with copper and zinc, as well as with pure copper and zinc cations, contains up to 148.0±14.9 pg interleukin-1β (IL-1β) per mL. The γ-globulins modified by copper or zinc binding induce, resp., 2.0 and 1.4-fold less IL-1β than intact proteins (significant by p = 0.1). Meanwhile, the copper-γ-globulin proved to be 2.7-fold more active (р < 0.01) IL-1β inducer, as a zinc-protein complex. Pure zinc ions did not induce IL-1β production, whereas induction rates with pure copper ions are 1.6-fold higher (p < 0.05) than with γ-globulin, and 1.45-fold more efficient that PHA stimulation (p < 0.1). A potential participation of copper and zinc cations bound to antibodies from the microenvironment and, thus, transforming Fc regions of antibody molecules, is discussed with regard of regulation of IL-1β production by human blood cells induced by activation of their Fc receptors. |
| format |
article |
| author |
S. B. Cheknev I. E. Efremova L. S. Piskovskaya E. N. Yushkovets A. A. Babajanz |
| author_facet |
S. B. Cheknev I. E. Efremova L. S. Piskovskaya E. N. Yushkovets A. A. Babajanz |
| author_sort |
S. B. Cheknev |
| title |
DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS |
| title_short |
DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS |
| title_full |
DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS |
| title_fullStr |
DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS |
| title_full_unstemmed |
DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS |
| title_sort |
detection of il-1β produced by human blood cells in presence of proteins from the γ-globulin fraction and their complexes with metals |
| publisher |
SPb RAACI |
| publishDate |
2014 |
| url |
https://doaj.org/article/f0bb304fb0584dddb2a14ede7e62e3d0 |
| work_keys_str_mv |
AT sbcheknev detectionofil1bproducedbyhumanbloodcellsinpresenceofproteinsfromthegglobulinfractionandtheircomplexeswithmetals AT ieefremova detectionofil1bproducedbyhumanbloodcellsinpresenceofproteinsfromthegglobulinfractionandtheircomplexeswithmetals AT lspiskovskaya detectionofil1bproducedbyhumanbloodcellsinpresenceofproteinsfromthegglobulinfractionandtheircomplexeswithmetals AT enyushkovets detectionofil1bproducedbyhumanbloodcellsinpresenceofproteinsfromthegglobulinfractionandtheircomplexeswithmetals AT aababajanz detectionofil1bproducedbyhumanbloodcellsinpresenceofproteinsfromthegglobulinfractionandtheircomplexeswithmetals |
| _version_ |
1718422517230600192 |