Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing

Abstract Bacterial cellulose (BC)/acrylic acid (AA) hydrogel has successfully been investigated as a wound dressing for partial-thickness burn wound. It is also a promising biomaterial cell carrier because it bears some resemblance to the natural soft tissue. This study assessed its ability to deliv...

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Autores principales:	Evelyn Yun Xi Loh, Najwa Mohamad, Mh Busra Fauzi, Min Hwei Ng, Shiow Fern Ng, Mohd Cairul Iqbal Mohd Amin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f0bd769c4471425a9b8a89cbb81765fc
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spelling	oai:doaj.org-article:f0bd769c4471425a9b8a89cbb81765fc2021-12-02T11:40:26ZDevelopment of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing10.1038/s41598-018-21174-72045-2322https://doaj.org/article/f0bd769c4471425a9b8a89cbb81765fc2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21174-7https://doaj.org/toc/2045-2322Abstract Bacterial cellulose (BC)/acrylic acid (AA) hydrogel has successfully been investigated as a wound dressing for partial-thickness burn wound. It is also a promising biomaterial cell carrier because it bears some resemblance to the natural soft tissue. This study assessed its ability to deliver human epidermal keratinocytes (EK) and dermal fibroblasts (DF) for the treatment of full-thickness skin lesions. In vitro studies demonstrated that BC/AA hydrogel had excellent cell attachment, maintained cell viability with limited migration, and allowed cell transfer. In vivo wound closure, histological, immunohistochemistry, and transmission electron microscopy evaluation revealed that hydrogel alone (HA) and hydrogel with cells (HC) accelerated wound healing compared to the untreated controls. Gross appearance and Masson’s trichrome staining indicated that HC was better than HA. This study suggests the potential application of BC/AA hydrogel with dual functions, as a cell carrier and wound dressing, to promote full-thickness wound healing.Evelyn Yun Xi LohNajwa MohamadMh Busra FauziMin Hwei NgShiow Fern NgMohd Cairul Iqbal Mohd AminNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Evelyn Yun Xi Loh Najwa Mohamad Mh Busra Fauzi Min Hwei Ng Shiow Fern Ng Mohd Cairul Iqbal Mohd Amin Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing
description	Abstract Bacterial cellulose (BC)/acrylic acid (AA) hydrogel has successfully been investigated as a wound dressing for partial-thickness burn wound. It is also a promising biomaterial cell carrier because it bears some resemblance to the natural soft tissue. This study assessed its ability to deliver human epidermal keratinocytes (EK) and dermal fibroblasts (DF) for the treatment of full-thickness skin lesions. In vitro studies demonstrated that BC/AA hydrogel had excellent cell attachment, maintained cell viability with limited migration, and allowed cell transfer. In vivo wound closure, histological, immunohistochemistry, and transmission electron microscopy evaluation revealed that hydrogel alone (HA) and hydrogel with cells (HC) accelerated wound healing compared to the untreated controls. Gross appearance and Masson’s trichrome staining indicated that HC was better than HA. This study suggests the potential application of BC/AA hydrogel with dual functions, as a cell carrier and wound dressing, to promote full-thickness wound healing.
format	article
author	Evelyn Yun Xi Loh Najwa Mohamad Mh Busra Fauzi Min Hwei Ng Shiow Fern Ng Mohd Cairul Iqbal Mohd Amin
author_facet	Evelyn Yun Xi Loh Najwa Mohamad Mh Busra Fauzi Min Hwei Ng Shiow Fern Ng Mohd Cairul Iqbal Mohd Amin
author_sort	Evelyn Yun Xi Loh
title	Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing
title_short	Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing
title_full	Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing
title_fullStr	Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing
title_full_unstemmed	Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing
title_sort	development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f0bd769c4471425a9b8a89cbb81765fc
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Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing

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