Topical application of nucleotides increase lysozyme levels in tears

Assumpta Peral1, Patricia Loma2, Benjamin Yerxa3, Jes&uacute;s Pintor21Departamento de &Oacute;ptica II (Optometr&iacute;a y Visi&oacute;n), Escuela Universitaria de &Oacute;ptica, Universidad Complutense de Madrid, Madrid, Spain; 2Departamento de Bioqu&am...

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Autores principales:	Assumpta Peral, Patricia Loma, Benjamin Yerxa, Jes&uacute;s Pintor
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/f0c5fdaba34540beaa695eda438c4115
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spelling	oai:doaj.org-article:f0c5fdaba34540beaa695eda438c41152021-12-02T01:00:07ZTopical application of nucleotides increase lysozyme levels in tears1177-54671177-5483https://doaj.org/article/f0c5fdaba34540beaa695eda438c41152008-07-01T00:00:00Zhttp://www.dovepress.com/topical-application-of-nucleotides-increase-lysozyme-levels-in-tears-a1778https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Assumpta Peral1, Patricia Loma2, Benjamin Yerxa3, Jes&uacute;s Pintor21Departamento de &Oacute;ptica II (Optometr&iacute;a y Visi&oacute;n), Escuela Universitaria de &Oacute;ptica, Universidad Complutense de Madrid, Madrid, Spain; 2Departamento de Bioqu&iacute;mica y Biolog&iacute;a Molecular IV, Escuela Universitaria de &Oacute;ptica, Universidad Complutense de Madrid, Madrid, Spain; 3Inspire Pharmaceuticals, Inc. Durham, North Carolina, USAAbstract: The present work studies the effects of topical application of nucleotides on rabbit tear lysozyme levels. Lysozyme values were determined by the diffusion in Agar method described by van Bijsterveld in 1974, and the protein amount was obtained by measuring the inhibitory halos around a Whatman n&ordm;1 paper disc of 5 mm in diameter. The tested nucleotides were UTP, Ap4A and Up4U. These compounds were topically instilled in a single-dose in one eye (with the contralateral eye as a control) and the lysozyme halos were measured along 5 hours. The obtained results showed an increase in the lysozyme concentrations of 67%, 93%, and 119% for UTP, Ap4A, and Up4U, respectively, over the basal levels of lysozyme. For this reason, we suggest these molecules as a potential treatment for the reinforcement of the tear film barrier against ocular infection.Keywords: lysozyme, nucleotides, tear film, ocular infection, ocular protection, ocular surface Assumpta PeralPatricia LomaBenjamin YerxaJes&uacute;s PintorDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2008, Iss Issue 2, Pp 261-267 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Assumpta Peral Patricia Loma Benjamin Yerxa Jes&uacute;s Pintor Topical application of nucleotides increase lysozyme levels in tears
description	Assumpta Peral1, Patricia Loma2, Benjamin Yerxa3, Jes&uacute;s Pintor21Departamento de &Oacute;ptica II (Optometr&iacute;a y Visi&oacute;n), Escuela Universitaria de &Oacute;ptica, Universidad Complutense de Madrid, Madrid, Spain; 2Departamento de Bioqu&iacute;mica y Biolog&iacute;a Molecular IV, Escuela Universitaria de &Oacute;ptica, Universidad Complutense de Madrid, Madrid, Spain; 3Inspire Pharmaceuticals, Inc. Durham, North Carolina, USAAbstract: The present work studies the effects of topical application of nucleotides on rabbit tear lysozyme levels. Lysozyme values were determined by the diffusion in Agar method described by van Bijsterveld in 1974, and the protein amount was obtained by measuring the inhibitory halos around a Whatman n&ordm;1 paper disc of 5 mm in diameter. The tested nucleotides were UTP, Ap4A and Up4U. These compounds were topically instilled in a single-dose in one eye (with the contralateral eye as a control) and the lysozyme halos were measured along 5 hours. The obtained results showed an increase in the lysozyme concentrations of 67%, 93%, and 119% for UTP, Ap4A, and Up4U, respectively, over the basal levels of lysozyme. For this reason, we suggest these molecules as a potential treatment for the reinforcement of the tear film barrier against ocular infection.Keywords: lysozyme, nucleotides, tear film, ocular infection, ocular protection, ocular surface
format	article
author	Assumpta Peral Patricia Loma Benjamin Yerxa Jes&uacute;s Pintor
author_facet	Assumpta Peral Patricia Loma Benjamin Yerxa Jes&uacute;s Pintor
author_sort	Assumpta Peral
title	Topical application of nucleotides increase lysozyme levels in tears
title_short	Topical application of nucleotides increase lysozyme levels in tears
title_full	Topical application of nucleotides increase lysozyme levels in tears
title_fullStr	Topical application of nucleotides increase lysozyme levels in tears
title_full_unstemmed	Topical application of nucleotides increase lysozyme levels in tears
title_sort	topical application of nucleotides increase lysozyme levels in tears
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/f0c5fdaba34540beaa695eda438c4115
work_keys_str_mv	AT assumptaperal topicalapplicationofnucleotidesincreaselysozymelevelsintears AT patricialoma topicalapplicationofnucleotidesincreaselysozymelevelsintears AT benjaminyerxa topicalapplicationofnucleotidesincreaselysozymelevelsintears AT jesampuacutespintor topicalapplicationofnucleotidesincreaselysozymelevelsintears
_version_	1718403412946583552

Topical application of nucleotides increase lysozyme levels in tears

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