Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans.
Improved genome engineering methods that enable automation of large and precise edits are essential for systematic investigations of genome function. We adapted peel-1 negative selection to an optimized Dual-Marker Selection (DMS) cassette protocol for CRISPR-Cas9 genome engineering in Caenorhabditi...
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| Main Authors: | , , , |
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| Format: | article |
| Language: | EN |
| Published: |
Public Library of Science (PLoS)
2020
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| Subjects: | |
| Online Access: | https://doaj.org/article/f0cebc28659b427cbe8025590b05f26a |
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