Micro RNAs of Epstein-Barr virus promote cell cycle progression and prevent apoptosis of primary human B cells.

Cellular and viral microRNAs (miRNAs) are involved in many different processes of key importance and more than 10,000 miRNAs have been identified so far. In general, relatively little is known about their biological functions in mammalian cells because their phenotypic effects are often mild and man...

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Autores principales:	Eri Seto, Andreas Moosmann, Sebastian Grömminger, Nicole Walz, Adam Grundhoff, Wolfgang Hammerschmidt
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f0cfdea179c64fa78ce73e6713729534
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spelling	oai:doaj.org-article:f0cfdea179c64fa78ce73e67137295342021-11-18T06:01:43ZMicro RNAs of Epstein-Barr virus promote cell cycle progression and prevent apoptosis of primary human B cells.1553-73661553-737410.1371/journal.ppat.1001063https://doaj.org/article/f0cfdea179c64fa78ce73e67137295342010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20808852/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Cellular and viral microRNAs (miRNAs) are involved in many different processes of key importance and more than 10,000 miRNAs have been identified so far. In general, relatively little is known about their biological functions in mammalian cells because their phenotypic effects are often mild and many of their targets still await identification. The recent discovery that Epstein-Barr virus (EBV) and other herpesviruses produce their own, barely conserved sets of miRNAs suggests that these viruses usurp the host RNA silencing machinery to their advantage in contrast to the antiviral roles of RNA silencing in plants and insects. We have systematically introduced mutations in EBV's precursor miRNA transcripts to prevent their subsequent processing into mature viral miRNAs. Phenotypic analyses of these mutant derivatives of EBV revealed that the viral miRNAs of the BHRF1 locus inhibit apoptosis and favor cell cycle progression and proliferation during the early phase of infected human primary B cells. Our findings also indicate that EBV's miRNAs are not needed to control the exit from latency. The phenotypes of viral miRNAs uncovered by this genetic analysis indicate that they contribute to EBV-associated cellular transformation rather than regulate viral genes of EBV's lytic phase.Eri SetoAndreas MoosmannSebastian GrömmingerNicole WalzAdam GrundhoffWolfgang HammerschmidtPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 8, p e1001063 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Eri Seto Andreas Moosmann Sebastian Grömminger Nicole Walz Adam Grundhoff Wolfgang Hammerschmidt Micro RNAs of Epstein-Barr virus promote cell cycle progression and prevent apoptosis of primary human B cells.
description	Cellular and viral microRNAs (miRNAs) are involved in many different processes of key importance and more than 10,000 miRNAs have been identified so far. In general, relatively little is known about their biological functions in mammalian cells because their phenotypic effects are often mild and many of their targets still await identification. The recent discovery that Epstein-Barr virus (EBV) and other herpesviruses produce their own, barely conserved sets of miRNAs suggests that these viruses usurp the host RNA silencing machinery to their advantage in contrast to the antiviral roles of RNA silencing in plants and insects. We have systematically introduced mutations in EBV's precursor miRNA transcripts to prevent their subsequent processing into mature viral miRNAs. Phenotypic analyses of these mutant derivatives of EBV revealed that the viral miRNAs of the BHRF1 locus inhibit apoptosis and favor cell cycle progression and proliferation during the early phase of infected human primary B cells. Our findings also indicate that EBV's miRNAs are not needed to control the exit from latency. The phenotypes of viral miRNAs uncovered by this genetic analysis indicate that they contribute to EBV-associated cellular transformation rather than regulate viral genes of EBV's lytic phase.
format	article
author	Eri Seto Andreas Moosmann Sebastian Grömminger Nicole Walz Adam Grundhoff Wolfgang Hammerschmidt
author_facet	Eri Seto Andreas Moosmann Sebastian Grömminger Nicole Walz Adam Grundhoff Wolfgang Hammerschmidt
author_sort	Eri Seto
title	Micro RNAs of Epstein-Barr virus promote cell cycle progression and prevent apoptosis of primary human B cells.
title_short	Micro RNAs of Epstein-Barr virus promote cell cycle progression and prevent apoptosis of primary human B cells.
title_full	Micro RNAs of Epstein-Barr virus promote cell cycle progression and prevent apoptosis of primary human B cells.
title_fullStr	Micro RNAs of Epstein-Barr virus promote cell cycle progression and prevent apoptosis of primary human B cells.
title_full_unstemmed	Micro RNAs of Epstein-Barr virus promote cell cycle progression and prevent apoptosis of primary human B cells.
title_sort	micro rnas of epstein-barr virus promote cell cycle progression and prevent apoptosis of primary human b cells.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/f0cfdea179c64fa78ce73e6713729534
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Micro RNAs of Epstein-Barr virus promote cell cycle progression and prevent apoptosis of primary human B cells.

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