Microsatellite markers for multiple Pocillopora genetic lineages offer new insights about coral populations

Abstract Population genetics of the coral genus Pocillopora have been more intensively studied than those of any other reef-building taxon. However, recent investigations have revealed that the current morphological classification is inadequate to represent genetic lineages. In this study, we isolat...

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Autores principales:	Yuichi Nakajima, Patricia H. Wepfer, Shohei Suzuki, Yuna Zayasu, Chuya Shinzato, Noriyuki Satoh, Satoshi Mitarai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f0d495f63bf14d8b975925a19d5dbc4b
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spelling	oai:doaj.org-article:f0d495f63bf14d8b975925a19d5dbc4b2021-12-02T16:06:43ZMicrosatellite markers for multiple Pocillopora genetic lineages offer new insights about coral populations10.1038/s41598-017-06776-x2045-2322https://doaj.org/article/f0d495f63bf14d8b975925a19d5dbc4b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06776-xhttps://doaj.org/toc/2045-2322Abstract Population genetics of the coral genus Pocillopora have been more intensively studied than those of any other reef-building taxon. However, recent investigations have revealed that the current morphological classification is inadequate to represent genetic lineages. In this study, we isolated and characterized novel microsatellite loci from morphological Pocillopora meandrina (Type 1) and Pocillopora acuta (Type 5). Furthermore, we characterized previously reported microsatellite loci. A total of 27 loci (13 novel loci) proved useful for population genetic analyses at two sites in the Ryukyu Archipelago, in the northwestern Pacific. Clonal diversity differed in each genetic lineage. Genetic structure suggested by microsatellites corresponded to clusters in a phylogenetic tree constructed from a mitochondrial open reading frame (mtORF). In addition, we found an unknown mitochondrial haplotype of this mtORF. These microsatellite loci will be useful for studies of connectivity and genetic diversity of Pocillopora populations, and will also support coral reef conservation.Yuichi NakajimaPatricia H. WepferShohei SuzukiYuna ZayasuChuya ShinzatoNoriyuki SatohSatoshi MitaraiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuichi Nakajima Patricia H. Wepfer Shohei Suzuki Yuna Zayasu Chuya Shinzato Noriyuki Satoh Satoshi Mitarai Microsatellite markers for multiple Pocillopora genetic lineages offer new insights about coral populations
description	Abstract Population genetics of the coral genus Pocillopora have been more intensively studied than those of any other reef-building taxon. However, recent investigations have revealed that the current morphological classification is inadequate to represent genetic lineages. In this study, we isolated and characterized novel microsatellite loci from morphological Pocillopora meandrina (Type 1) and Pocillopora acuta (Type 5). Furthermore, we characterized previously reported microsatellite loci. A total of 27 loci (13 novel loci) proved useful for population genetic analyses at two sites in the Ryukyu Archipelago, in the northwestern Pacific. Clonal diversity differed in each genetic lineage. Genetic structure suggested by microsatellites corresponded to clusters in a phylogenetic tree constructed from a mitochondrial open reading frame (mtORF). In addition, we found an unknown mitochondrial haplotype of this mtORF. These microsatellite loci will be useful for studies of connectivity and genetic diversity of Pocillopora populations, and will also support coral reef conservation.
format	article
author	Yuichi Nakajima Patricia H. Wepfer Shohei Suzuki Yuna Zayasu Chuya Shinzato Noriyuki Satoh Satoshi Mitarai
author_facet	Yuichi Nakajima Patricia H. Wepfer Shohei Suzuki Yuna Zayasu Chuya Shinzato Noriyuki Satoh Satoshi Mitarai
author_sort	Yuichi Nakajima
title	Microsatellite markers for multiple Pocillopora genetic lineages offer new insights about coral populations
title_short	Microsatellite markers for multiple Pocillopora genetic lineages offer new insights about coral populations
title_full	Microsatellite markers for multiple Pocillopora genetic lineages offer new insights about coral populations
title_fullStr	Microsatellite markers for multiple Pocillopora genetic lineages offer new insights about coral populations
title_full_unstemmed	Microsatellite markers for multiple Pocillopora genetic lineages offer new insights about coral populations
title_sort	microsatellite markers for multiple pocillopora genetic lineages offer new insights about coral populations
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f0d495f63bf14d8b975925a19d5dbc4b
work_keys_str_mv	AT yuichinakajima microsatellitemarkersformultiplepocilloporageneticlineagesoffernewinsightsaboutcoralpopulations AT patriciahwepfer microsatellitemarkersformultiplepocilloporageneticlineagesoffernewinsightsaboutcoralpopulations AT shoheisuzuki microsatellitemarkersformultiplepocilloporageneticlineagesoffernewinsightsaboutcoralpopulations AT yunazayasu microsatellitemarkersformultiplepocilloporageneticlineagesoffernewinsightsaboutcoralpopulations AT chuyashinzato microsatellitemarkersformultiplepocilloporageneticlineagesoffernewinsightsaboutcoralpopulations AT noriyukisatoh microsatellitemarkersformultiplepocilloporageneticlineagesoffernewinsightsaboutcoralpopulations AT satoshimitarai microsatellitemarkersformultiplepocilloporageneticlineagesoffernewinsightsaboutcoralpopulations
_version_	1718384940238766080

Microsatellite markers for multiple Pocillopora genetic lineages offer new insights about coral populations

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