Non-ionic surfactant vesicles simultaneously enhance antitumor activity and reduce the toxicity of cantharidin

Wei Han,1,* Shengpeng Wang,2,* Rixin Liang,1 Lan Wang,1 Meiwan Chen,2 Hui Li,1 Yitao Wang1,2 1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, People’s Republic of China; 2State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chi...

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Autores principales:	Han W, Wang S, Liang R, Wang L, Chen M, Li H, Wang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f0d659ee18df4c7998400b284eaeb09e
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spelling	oai:doaj.org-article:f0d659ee18df4c7998400b284eaeb09e2021-12-02T07:28:28ZNon-ionic surfactant vesicles simultaneously enhance antitumor activity and reduce the toxicity of cantharidin1176-91141178-2013https://doaj.org/article/f0d659ee18df4c7998400b284eaeb09e2013-06-01T00:00:00Zhttp://www.dovepress.com/non-ionic-surfactant-vesicles-simultaneously-enhance-antitumor-activit-a13343https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Wei Han,1,* Shengpeng Wang,2,* Rixin Liang,1 Lan Wang,1 Meiwan Chen,2 Hui Li,1 Yitao Wang1,2 1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, People’s Republic of China; 2State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, People’s Republic of China *These authors contributed equally to this work Objective: The objective of the present study was to prepare cantharidin-entrapped non-ionic surfactant vesicles (CTD-NSVs) and evaluate their potential in enhancing the antitumor activities and reducing CTD’s toxicity. Methods and results: CTD-NSVs were prepared by injection method. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry analysis showed that CTD-NSVs could significantly enhance in vitro toxicity against human breast cancer cell line MCF-7 and induce more significant cell-cycle arrest in G0/G1 phase. Moreover, Hoechst 33342 staining implicated that CTD-NSVs induced higher apoptotic rates in MCF-7 cells than free CTD solution. In vivo therapeutic efficacy was investigated in imprinting control region mice bearing mouse sarcoma S180. Mice treated with 1.0 mg/kg CTD-NSVs showed the most powerful antitumor activity, with an inhibition rate of 52.76%, which was significantly higher than that of cyclophosphamide (35 mg/kg, 40.23%) and the same concentration of free CTD (1.0 mg/kg, 31.05%). In addition, the acute toxicity and liver toxicity of CTD were also distinctly decreased via encapsulating into NSVs. Conclusion: Our results revealed that NSVs could be a promising delivery system for enhancing the antitumor activity and simultaneously reducing the toxicity of CTD. Keywords: cantharidin, non-ionic surfactant vesicle, toxicity, antitumor activityHan WWang SLiang RWang LChen MLi HWang YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2187-2196 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Han W Wang S Liang R Wang L Chen M Li H Wang Y Non-ionic surfactant vesicles simultaneously enhance antitumor activity and reduce the toxicity of cantharidin
description	Wei Han,1,* Shengpeng Wang,2,* Rixin Liang,1 Lan Wang,1 Meiwan Chen,2 Hui Li,1 Yitao Wang1,2 1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, People’s Republic of China; 2State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, People’s Republic of China *These authors contributed equally to this work Objective: The objective of the present study was to prepare cantharidin-entrapped non-ionic surfactant vesicles (CTD-NSVs) and evaluate their potential in enhancing the antitumor activities and reducing CTD’s toxicity. Methods and results: CTD-NSVs were prepared by injection method. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry analysis showed that CTD-NSVs could significantly enhance in vitro toxicity against human breast cancer cell line MCF-7 and induce more significant cell-cycle arrest in G0/G1 phase. Moreover, Hoechst 33342 staining implicated that CTD-NSVs induced higher apoptotic rates in MCF-7 cells than free CTD solution. In vivo therapeutic efficacy was investigated in imprinting control region mice bearing mouse sarcoma S180. Mice treated with 1.0 mg/kg CTD-NSVs showed the most powerful antitumor activity, with an inhibition rate of 52.76%, which was significantly higher than that of cyclophosphamide (35 mg/kg, 40.23%) and the same concentration of free CTD (1.0 mg/kg, 31.05%). In addition, the acute toxicity and liver toxicity of CTD were also distinctly decreased via encapsulating into NSVs. Conclusion: Our results revealed that NSVs could be a promising delivery system for enhancing the antitumor activity and simultaneously reducing the toxicity of CTD. Keywords: cantharidin, non-ionic surfactant vesicle, toxicity, antitumor activity
format	article
author	Han W Wang S Liang R Wang L Chen M Li H Wang Y
author_facet	Han W Wang S Liang R Wang L Chen M Li H Wang Y
author_sort	Han W
title	Non-ionic surfactant vesicles simultaneously enhance antitumor activity and reduce the toxicity of cantharidin
title_short	Non-ionic surfactant vesicles simultaneously enhance antitumor activity and reduce the toxicity of cantharidin
title_full	Non-ionic surfactant vesicles simultaneously enhance antitumor activity and reduce the toxicity of cantharidin
title_fullStr	Non-ionic surfactant vesicles simultaneously enhance antitumor activity and reduce the toxicity of cantharidin
title_full_unstemmed	Non-ionic surfactant vesicles simultaneously enhance antitumor activity and reduce the toxicity of cantharidin
title_sort	non-ionic surfactant vesicles simultaneously enhance antitumor activity and reduce the toxicity of cantharidin
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/f0d659ee18df4c7998400b284eaeb09e
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Non-ionic surfactant vesicles simultaneously enhance antitumor activity and reduce the toxicity of cantharidin

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