Main Pathways and Ion Channels Differentially Expressed in the Transcriptome of Male and Female Adult Angiostrongylus cantonensis using a Deep Sequencing Approach

Background: The adult stage is an important period in the life cycle of Angiostrongylus cantonensis, as it is at this stage that male and female worms produce thousands of fertilized eggs daily. Methods: To explore the transcriptional details of adult male and female A. cantonensis, three groups...

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Autores principales:	Yue Guo, Hong Chang Zhou, Hai Yan Dong, Yun Liang Yao, Bo Ying Xu, Yu Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Tehran University of Medical Sciences 2021
Materias:	angiostrongylus cantonensis adult stage transcriptome calcium signaling pathway Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/f0df955050f547949a3f3b9ee4126273
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spelling	oai:doaj.org-article:f0df955050f547949a3f3b9ee41262732021-11-29T07:18:54ZMain Pathways and Ion Channels Differentially Expressed in the Transcriptome of Male and Female Adult Angiostrongylus cantonensis using a Deep Sequencing Approach1735-70202008-238Xhttps://doaj.org/article/f0df955050f547949a3f3b9ee41262732021-11-01T00:00:00Zhttps://ijpa.tums.ac.ir/index.php/ijpa/article/view/3132https://doaj.org/toc/1735-7020https://doaj.org/toc/2008-238XBackground: The adult stage is an important period in the life cycle of Angiostrongylus cantonensis, as it is at this stage that male and female worms produce thousands of fertilized eggs daily. Methods: To explore the transcriptional details of adult male and female A. cantonensis, three groups of male and female adult worms were collected, and their transcriptome profiles were analyzed using an Illumina next-generation sequencing platform. A total of 283,910,174 clean reads were obtained, and 137,626 unigenes and 237,059 transcripts were then generated. Unigenes were successfully annotated by querying the Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG), NCBI non-redundant protein sequences (NR), PFAM, STRING, and SWISS-PROT databases. Then, differentially expressed genes (DEGs) between the 2 genders were identified. The GO and KEGG databases were used for DEG annotation, and a number of DEG annotations were enriched. Results: The results obtained from querying DEGs using the GO and KEGG databases revealed that male and female adult worms exhibited differences in metabolism and production. Protein phosphorylation, ion transport, and calcium transport were all significantly enriched according to GO annotation. A number of other pathways were also enriched according to KEGG enrichment annotation, including the pentose phosphate pathway, nitrogen metabolism, oocyte meiosis pathway, neuroactive ligand-receptor interaction, calcium signaling pathway, transforming growth factor β (TGF-β) signaling pathway etc. Conclusion: We hypothesized that the nervous system of the worm plays a key role in the physiological regulation of adult A. cantonensis, and based on this, the function of the calcium-signaling pathway should be investigated.Yue GuoHong Chang ZhouHai Yan DongYun Liang YaoBo Ying XuYu ZhaoTehran University of Medical Sciencesarticleangiostrongylus cantonensisadult stagetranscriptomecalcium signaling pathwayInfectious and parasitic diseasesRC109-216ENIranian Journal of Parasitology, Vol 16, Iss 4, Pp 610-620 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	angiostrongylus cantonensis adult stage transcriptome calcium signaling pathway Infectious and parasitic diseases RC109-216
spellingShingle	angiostrongylus cantonensis adult stage transcriptome calcium signaling pathway Infectious and parasitic diseases RC109-216 Yue Guo Hong Chang Zhou Hai Yan Dong Yun Liang Yao Bo Ying Xu Yu Zhao Main Pathways and Ion Channels Differentially Expressed in the Transcriptome of Male and Female Adult Angiostrongylus cantonensis using a Deep Sequencing Approach
description	Background: The adult stage is an important period in the life cycle of Angiostrongylus cantonensis, as it is at this stage that male and female worms produce thousands of fertilized eggs daily. Methods: To explore the transcriptional details of adult male and female A. cantonensis, three groups of male and female adult worms were collected, and their transcriptome profiles were analyzed using an Illumina next-generation sequencing platform. A total of 283,910,174 clean reads were obtained, and 137,626 unigenes and 237,059 transcripts were then generated. Unigenes were successfully annotated by querying the Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG), NCBI non-redundant protein sequences (NR), PFAM, STRING, and SWISS-PROT databases. Then, differentially expressed genes (DEGs) between the 2 genders were identified. The GO and KEGG databases were used for DEG annotation, and a number of DEG annotations were enriched. Results: The results obtained from querying DEGs using the GO and KEGG databases revealed that male and female adult worms exhibited differences in metabolism and production. Protein phosphorylation, ion transport, and calcium transport were all significantly enriched according to GO annotation. A number of other pathways were also enriched according to KEGG enrichment annotation, including the pentose phosphate pathway, nitrogen metabolism, oocyte meiosis pathway, neuroactive ligand-receptor interaction, calcium signaling pathway, transforming growth factor β (TGF-β) signaling pathway etc. Conclusion: We hypothesized that the nervous system of the worm plays a key role in the physiological regulation of adult A. cantonensis, and based on this, the function of the calcium-signaling pathway should be investigated.
format	article
author	Yue Guo Hong Chang Zhou Hai Yan Dong Yun Liang Yao Bo Ying Xu Yu Zhao
author_facet	Yue Guo Hong Chang Zhou Hai Yan Dong Yun Liang Yao Bo Ying Xu Yu Zhao
author_sort	Yue Guo
title	Main Pathways and Ion Channels Differentially Expressed in the Transcriptome of Male and Female Adult Angiostrongylus cantonensis using a Deep Sequencing Approach
title_short	Main Pathways and Ion Channels Differentially Expressed in the Transcriptome of Male and Female Adult Angiostrongylus cantonensis using a Deep Sequencing Approach
title_full	Main Pathways and Ion Channels Differentially Expressed in the Transcriptome of Male and Female Adult Angiostrongylus cantonensis using a Deep Sequencing Approach
title_fullStr	Main Pathways and Ion Channels Differentially Expressed in the Transcriptome of Male and Female Adult Angiostrongylus cantonensis using a Deep Sequencing Approach
title_full_unstemmed	Main Pathways and Ion Channels Differentially Expressed in the Transcriptome of Male and Female Adult Angiostrongylus cantonensis using a Deep Sequencing Approach
title_sort	main pathways and ion channels differentially expressed in the transcriptome of male and female adult angiostrongylus cantonensis using a deep sequencing approach
publisher	Tehran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/f0df955050f547949a3f3b9ee4126273
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Main Pathways and Ion Channels Differentially Expressed in the Transcriptome of Male and Female Adult Angiostrongylus cantonensis using a Deep Sequencing Approach

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