Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells

Ahmad Abolhasani,1 Davoud Biria,1 Hoda Abolhasani,2 Ali Zarrabi,1 Tahereh Komeili2 1Department of Biotechnology, University of Isfahan, Isfahan, Iran; 2Department of Physiology and Pharmacology, Qom University of Medical Sciences, Qom, IranCorrespondence: Davoud BiriaDepartment of Biotechnology, Uni...

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Autores principales:	Abolhasani A, Biria D, Abolhasani H, Zarrabi A, Komeili T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	glucose decorated nanoparticles plga chitosan glut tumor cells. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f0e51301b70743dda926145ca2cb27d4
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spelling	oai:doaj.org-article:f0e51301b70743dda926145ca2cb27d42021-12-02T05:26:53ZInvestigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells1178-2013https://doaj.org/article/f0e51301b70743dda926145ca2cb27d42019-12-01T00:00:00Zhttps://www.dovepress.com/investigation-of-the-role-of-glucose-decorated-chitosan-and-plga-nanop-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ahmad Abolhasani,1 Davoud Biria,1 Hoda Abolhasani,2 Ali Zarrabi,1 Tahereh Komeili2 1Department of Biotechnology, University of Isfahan, Isfahan, Iran; 2Department of Physiology and Pharmacology, Qom University of Medical Sciences, Qom, IranCorrespondence: Davoud BiriaDepartment of Biotechnology, University of Isfahan, P.O. Box: 8174673441, Isfahan, IranTel +983137934373Email d.biria@ast.ui.ac.irPurpose: Glucose decorated PLGA and chitosan nanoparticles (GPNPs and GCNPs) have been developed to examine the possibility of preventing the facilitated glucose transport to the cells through blocking the glucose transporters (Gluts) overexpressed by tumor cells.Methods: The MTT assay was used to assess the cytotoxicity towards human colon tumor (HT-29) cells in 72 hrs. Fluorescence microscopy was employed to confirm the attachment of GPNPs to the cells. Moreover, the GPNPs effects on the apoptotic rate of HT-29 cells were analyzed. Finally, the expression levels of GLUT-1 and GLUT-4 by real-time polymerase chain reaction (RT-PCR) were assayed to investigate the response of HT-29 cells to blocking their Gluts by GPNPs.Results: The stability studies showed that the synthesized complexes were mostly stable (more than 80%) at various temperatures (4 to 40ºC) and pH (5.4 to 7.4) conditions. Results indicated that the survival rate of the cells was decreased to 43% and 46% after treatment with GCNPs and GPNPs, respectively. Also, the apoptosis assay results showed that the percentage of viable cells reduced to 47% after GPNPs treatment. These observations were justified by the specific interactions between the glucose terminals and the cells Gluts which resulted in blocking the entries of nutrients to the cells. It was revealed that the GLUT-1 mRNA expression after the first 24 h of treatment by GPNPs was upregulated to more than 145%, while the direction was reversed after 72 h (expression less than 45%), which coincided with the cells death. In the first 24 h, the glucose deprivation stimulated the expression of Glut-1 while the apoptotic enzymes expression was dominant at the end of 72 h treatment time.Conclusion: Finally, it can be concluded that the glucose-nanoparticle complexes could be considered as promising agents in cancer therapy.Keywords: glucose decorated nanoparticles, PLGA, chitosan, glut, tumor cellsAbolhasani ABiria DAbolhasani HZarrabi AKomeili TDove Medical Pressarticleglucose decorated nanoparticlesplgachitosangluttumor cells.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9535-9546 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	glucose decorated nanoparticles plga chitosan glut tumor cells. Medicine (General) R5-920
spellingShingle	glucose decorated nanoparticles plga chitosan glut tumor cells. Medicine (General) R5-920 Abolhasani A Biria D Abolhasani H Zarrabi A Komeili T Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells
description	Ahmad Abolhasani,1 Davoud Biria,1 Hoda Abolhasani,2 Ali Zarrabi,1 Tahereh Komeili2 1Department of Biotechnology, University of Isfahan, Isfahan, Iran; 2Department of Physiology and Pharmacology, Qom University of Medical Sciences, Qom, IranCorrespondence: Davoud BiriaDepartment of Biotechnology, University of Isfahan, P.O. Box: 8174673441, Isfahan, IranTel +983137934373Email d.biria@ast.ui.ac.irPurpose: Glucose decorated PLGA and chitosan nanoparticles (GPNPs and GCNPs) have been developed to examine the possibility of preventing the facilitated glucose transport to the cells through blocking the glucose transporters (Gluts) overexpressed by tumor cells.Methods: The MTT assay was used to assess the cytotoxicity towards human colon tumor (HT-29) cells in 72 hrs. Fluorescence microscopy was employed to confirm the attachment of GPNPs to the cells. Moreover, the GPNPs effects on the apoptotic rate of HT-29 cells were analyzed. Finally, the expression levels of GLUT-1 and GLUT-4 by real-time polymerase chain reaction (RT-PCR) were assayed to investigate the response of HT-29 cells to blocking their Gluts by GPNPs.Results: The stability studies showed that the synthesized complexes were mostly stable (more than 80%) at various temperatures (4 to 40ºC) and pH (5.4 to 7.4) conditions. Results indicated that the survival rate of the cells was decreased to 43% and 46% after treatment with GCNPs and GPNPs, respectively. Also, the apoptosis assay results showed that the percentage of viable cells reduced to 47% after GPNPs treatment. These observations were justified by the specific interactions between the glucose terminals and the cells Gluts which resulted in blocking the entries of nutrients to the cells. It was revealed that the GLUT-1 mRNA expression after the first 24 h of treatment by GPNPs was upregulated to more than 145%, while the direction was reversed after 72 h (expression less than 45%), which coincided with the cells death. In the first 24 h, the glucose deprivation stimulated the expression of Glut-1 while the apoptotic enzymes expression was dominant at the end of 72 h treatment time.Conclusion: Finally, it can be concluded that the glucose-nanoparticle complexes could be considered as promising agents in cancer therapy.Keywords: glucose decorated nanoparticles, PLGA, chitosan, glut, tumor cells
format	article
author	Abolhasani A Biria D Abolhasani H Zarrabi A Komeili T
author_facet	Abolhasani A Biria D Abolhasani H Zarrabi A Komeili T
author_sort	Abolhasani A
title	Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells
title_short	Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells
title_full	Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells
title_fullStr	Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells
title_full_unstemmed	Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells
title_sort	investigation of the role of glucose decorated chitosan and plga nanoparticles as blocking agents to glucose transporters of tumor cells
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/f0e51301b70743dda926145ca2cb27d4
work_keys_str_mv	AT abolhasania investigationoftheroleofglucosedecoratedchitosanandplgananoparticlesasblockingagentstoglucosetransportersoftumorcells AT biriad investigationoftheroleofglucosedecoratedchitosanandplgananoparticlesasblockingagentstoglucosetransportersoftumorcells AT abolhasanih investigationoftheroleofglucosedecoratedchitosanandplgananoparticlesasblockingagentstoglucosetransportersoftumorcells AT zarrabia investigationoftheroleofglucosedecoratedchitosanandplgananoparticlesasblockingagentstoglucosetransportersoftumorcells AT komeilit investigationoftheroleofglucosedecoratedchitosanandplgananoparticlesasblockingagentstoglucosetransportersoftumorcells
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Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells

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