A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization

There is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of gold nanoparticle-based multilayer nanoconstructs...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anna V. Epanchintseva, Julia E. Poletaeva, Ilya S. Dovydenko, Boris P. Chelobanov, Dmitrii V. Pyshnyi, Elena I. Ryabchikova, Inna A. Pyshnaya
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gold nanoparticles siRNA noncovalent adsorption lipid enveloping multilayer nanoconstructs for siRNA preparation and purification Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/f0e5ea8054c849ac95ad2f10fdc5d8b2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f0e5ea8054c849ac95ad2f10fdc5d8b2
record_format	dspace
spelling	oai:doaj.org-article:f0e5ea8054c849ac95ad2f10fdc5d8b22021-11-25T18:29:43ZA Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization10.3390/nano111127752079-4991https://doaj.org/article/f0e5ea8054c849ac95ad2f10fdc5d8b22021-10-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2775https://doaj.org/toc/2079-4991There is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of gold nanoparticle-based multilayer nanoconstructs (MLNCs) for suppressing reporter protein synthesis. The present work is aimed at improving the quality of preparations of desired MLNCs, and for this purpose, optimal conditions for their multistep fabrication were found. All steps of this process and MLNC purification were verified using dynamic light scattering, transmission electron microscopy, and UV-Vis spectroscopy. Factors influencing the efficiency of nanocomposite assembly, colloidal stability, and purification quality were identified. These data made it possible to optimize the fabrication of target MLNCs bearing small interfering RNA and to substantially improve end product quality via an increase in its homogeneity and a decrease in the amount of incomplete nanoconstructs. We believe that the proposed approaches and methods will be useful for researchers working with lipid nanoconstructs.Anna V. EpanchintsevaJulia E. PoletaevaIlya S. DovydenkoBoris P. ChelobanovDmitrii V. PyshnyiElena I. RyabchikovaInna A. PyshnayaMDPI AGarticlegold nanoparticlessiRNAnoncovalent adsorptionlipid envelopingmultilayer nanoconstructs for siRNA preparation and purificationChemistryQD1-999ENNanomaterials, Vol 11, Iss 2775, p 2775 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gold nanoparticles siRNA noncovalent adsorption lipid enveloping multilayer nanoconstructs for siRNA preparation and purification Chemistry QD1-999
spellingShingle	gold nanoparticles siRNA noncovalent adsorption lipid enveloping multilayer nanoconstructs for siRNA preparation and purification Chemistry QD1-999 Anna V. Epanchintseva Julia E. Poletaeva Ilya S. Dovydenko Boris P. Chelobanov Dmitrii V. Pyshnyi Elena I. Ryabchikova Inna A. Pyshnaya A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
description	There is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of gold nanoparticle-based multilayer nanoconstructs (MLNCs) for suppressing reporter protein synthesis. The present work is aimed at improving the quality of preparations of desired MLNCs, and for this purpose, optimal conditions for their multistep fabrication were found. All steps of this process and MLNC purification were verified using dynamic light scattering, transmission electron microscopy, and UV-Vis spectroscopy. Factors influencing the efficiency of nanocomposite assembly, colloidal stability, and purification quality were identified. These data made it possible to optimize the fabrication of target MLNCs bearing small interfering RNA and to substantially improve end product quality via an increase in its homogeneity and a decrease in the amount of incomplete nanoconstructs. We believe that the proposed approaches and methods will be useful for researchers working with lipid nanoconstructs.
format	article
author	Anna V. Epanchintseva Julia E. Poletaeva Ilya S. Dovydenko Boris P. Chelobanov Dmitrii V. Pyshnyi Elena I. Ryabchikova Inna A. Pyshnaya
author_facet	Anna V. Epanchintseva Julia E. Poletaeva Ilya S. Dovydenko Boris P. Chelobanov Dmitrii V. Pyshnyi Elena I. Ryabchikova Inna A. Pyshnaya
author_sort	Anna V. Epanchintseva
title	A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
title_short	A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
title_full	A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
title_fullStr	A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
title_full_unstemmed	A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
title_sort	lipid-coated nanoconstruct composed of gold nanoparticles noncovalently coated with small interfering rna: preparation, purification and characterization
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f0e5ea8054c849ac95ad2f10fdc5d8b2
work_keys_str_mv	AT annavepanchintseva alipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT juliaepoletaeva alipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT ilyasdovydenko alipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT borispchelobanov alipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT dmitriivpyshnyi alipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT elenairyabchikova alipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT innaapyshnaya alipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT annavepanchintseva lipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT juliaepoletaeva lipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT ilyasdovydenko lipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT borispchelobanov lipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT dmitriivpyshnyi lipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT elenairyabchikova lipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization AT innaapyshnaya lipidcoatednanoconstructcomposedofgoldnanoparticlesnoncovalentlycoatedwithsmallinterferingrnapreparationpurificationandcharacterization
_version_	1718411111217233920

A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization

Ejemplares similares