Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model

Weiguo Xu1, Cornelia Ganz2, Ulf Weber2, Martin Adam2, Gerd Holzh&uuml;ter2, Daniel Wolter3, Bernhard Frerich3, Brigitte Vollmar1, Thomas Gerber21Institute for Experimental Surgery, 2Institute of Physics, 3Department of Oral, Maxillofacial and Plastic Surgery, University of Rostock, Rostock,...

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Autores principales:	Xu W, Ganz C, Weber U, Adam M, Holzh&uuml;ter G, Wolter D, Frerich B, Vollmar B, Gerber T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f0e8f69a7e064705a0b435e74e1fa605
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spelling	oai:doaj.org-article:f0e8f69a7e064705a0b435e74e1fa6052021-12-02T07:28:28ZEvaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model1176-91141178-2013https://doaj.org/article/f0e8f69a7e064705a0b435e74e1fa6052011-08-01T00:00:00Zhttp://www.dovepress.com/evaluation-of-injectable-silica-embedded-nanohydroxyapatite-bone-subst-a7999https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Weiguo Xu1, Cornelia Ganz2, Ulf Weber2, Martin Adam2, Gerd Holzh&uuml;ter2, Daniel Wolter3, Bernhard Frerich3, Brigitte Vollmar1, Thomas Gerber21Institute for Experimental Surgery, 2Institute of Physics, 3Department of Oral, Maxillofacial and Plastic Surgery, University of Rostock, Rostock, GermanyAbstract: In clinical practice, vertebral compression fractures occur after trauma and osteoporosis. Kyphoplasty is a minimally invasive procedure using bone filler material for the treatment of such fractures. A full synthetic injectable bone substitute (SIBS) was manufactured by means of spray drying. The aim of this study was to characterize the SIBS and to analyze the remodelling process during degradation of the biomaterial and new bone formation after implantation. SIBS is an aqueous suspension of donut-like microparticles. These microparticles consist of nanocrystallites of synthetic hydroxyapatite embedded in amorphous silica gel. After implantation of SIBS in a proximal tibial diaphyseal defect in 52 rats, grafts were harvested for subsequent analysis on different days. Newly formed bone originating from endosteum was observed on day 6. Hematomas in the medullary space and cortical wounds disappeared on day 12. The wound region was completely replaced by a composite of newly formed cancellous bone, extracellular matrix, and SIBS. At day 63 the cortical defect was fully healed by bone, while newly formed bone in the medullary space almost disappeared and was replaced with bone marrow. In conclusion, SIBS demonstrated a unique structure with osteoinductive and bioresorbable properties, which induced fast bone regeneration. Therefore, a clinical application of SIBS for kyphoplasty is promising.Keywords: bone remodelling, electron microscopy, histomorphometry, nanotechnology, tissue engineeringXu WGanz CWeber UAdam MHolzh&uuml;ter GWolter DFrerich BVollmar BGerber TDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1543-1552 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Xu W Ganz C Weber U Adam M Holzh&uuml;ter G Wolter D Frerich B Vollmar B Gerber T Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model
description	Weiguo Xu1, Cornelia Ganz2, Ulf Weber2, Martin Adam2, Gerd Holzh&uuml;ter2, Daniel Wolter3, Bernhard Frerich3, Brigitte Vollmar1, Thomas Gerber21Institute for Experimental Surgery, 2Institute of Physics, 3Department of Oral, Maxillofacial and Plastic Surgery, University of Rostock, Rostock, GermanyAbstract: In clinical practice, vertebral compression fractures occur after trauma and osteoporosis. Kyphoplasty is a minimally invasive procedure using bone filler material for the treatment of such fractures. A full synthetic injectable bone substitute (SIBS) was manufactured by means of spray drying. The aim of this study was to characterize the SIBS and to analyze the remodelling process during degradation of the biomaterial and new bone formation after implantation. SIBS is an aqueous suspension of donut-like microparticles. These microparticles consist of nanocrystallites of synthetic hydroxyapatite embedded in amorphous silica gel. After implantation of SIBS in a proximal tibial diaphyseal defect in 52 rats, grafts were harvested for subsequent analysis on different days. Newly formed bone originating from endosteum was observed on day 6. Hematomas in the medullary space and cortical wounds disappeared on day 12. The wound region was completely replaced by a composite of newly formed cancellous bone, extracellular matrix, and SIBS. At day 63 the cortical defect was fully healed by bone, while newly formed bone in the medullary space almost disappeared and was replaced with bone marrow. In conclusion, SIBS demonstrated a unique structure with osteoinductive and bioresorbable properties, which induced fast bone regeneration. Therefore, a clinical application of SIBS for kyphoplasty is promising.Keywords: bone remodelling, electron microscopy, histomorphometry, nanotechnology, tissue engineering
format	article
author	Xu W Ganz C Weber U Adam M Holzh&uuml;ter G Wolter D Frerich B Vollmar B Gerber T
author_facet	Xu W Ganz C Weber U Adam M Holzh&uuml;ter G Wolter D Frerich B Vollmar B Gerber T
author_sort	Xu W
title	Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model
title_short	Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model
title_full	Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model
title_fullStr	Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model
title_full_unstemmed	Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model
title_sort	evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/f0e8f69a7e064705a0b435e74e1fa605
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Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model

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