Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry

Abstract Measuring optically detected magnetic resonance (ODMR) of diamond nitrogen vacancy centers significantly depends on the photon detectors used. We study camera-based wide-field ODMR measurements to examine the performance in thermometry by comparing the results to those of the confocal-based...

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Autores principales:	Yushi Nishimura, Keisuke Oshimi, Yumi Umehara, Yuka Kumon, Kazu Miyaji, Hiroshi Yukawa, Yutaka Shikano, Tsutomu Matsubara, Masazumi Fujiwara, Yoshinobu Baba, Yoshio Teki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f0f59cf4b73b43128d3fd385a7a7e7ec
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spelling	oai:doaj.org-article:f0f59cf4b73b43128d3fd385a7a7e7ec2021-12-02T12:11:35ZWide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry10.1038/s41598-021-83285-y2045-2322https://doaj.org/article/f0f59cf4b73b43128d3fd385a7a7e7ec2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83285-yhttps://doaj.org/toc/2045-2322Abstract Measuring optically detected magnetic resonance (ODMR) of diamond nitrogen vacancy centers significantly depends on the photon detectors used. We study camera-based wide-field ODMR measurements to examine the performance in thermometry by comparing the results to those of the confocal-based ODMR detection. We show that the temperature sensitivity of the camera-based measurements can be as high as that of the confocal detection and that possible artifacts of the ODMR shift are produced owing to the complexity of the camera-based measurements. Although measurements from wide-field ODMR of nanodiamonds in living cells can provide temperature precisions consistent with those of confocal detection, the technique requires the integration of rapid ODMR measurement protocols for better precisions. Our results can aid the development of camera-based real-time large-area spin-based thermometry of living cells.Yushi NishimuraKeisuke OshimiYumi UmeharaYuka KumonKazu MiyajiHiroshi YukawaYutaka ShikanoTsutomu MatsubaraMasazumi FujiwaraYoshinobu BabaYoshio TekiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yushi Nishimura Keisuke Oshimi Yumi Umehara Yuka Kumon Kazu Miyaji Hiroshi Yukawa Yutaka Shikano Tsutomu Matsubara Masazumi Fujiwara Yoshinobu Baba Yoshio Teki Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry
description	Abstract Measuring optically detected magnetic resonance (ODMR) of diamond nitrogen vacancy centers significantly depends on the photon detectors used. We study camera-based wide-field ODMR measurements to examine the performance in thermometry by comparing the results to those of the confocal-based ODMR detection. We show that the temperature sensitivity of the camera-based measurements can be as high as that of the confocal detection and that possible artifacts of the ODMR shift are produced owing to the complexity of the camera-based measurements. Although measurements from wide-field ODMR of nanodiamonds in living cells can provide temperature precisions consistent with those of confocal detection, the technique requires the integration of rapid ODMR measurement protocols for better precisions. Our results can aid the development of camera-based real-time large-area spin-based thermometry of living cells.
format	article
author	Yushi Nishimura Keisuke Oshimi Yumi Umehara Yuka Kumon Kazu Miyaji Hiroshi Yukawa Yutaka Shikano Tsutomu Matsubara Masazumi Fujiwara Yoshinobu Baba Yoshio Teki
author_facet	Yushi Nishimura Keisuke Oshimi Yumi Umehara Yuka Kumon Kazu Miyaji Hiroshi Yukawa Yutaka Shikano Tsutomu Matsubara Masazumi Fujiwara Yoshinobu Baba Yoshio Teki
author_sort	Yushi Nishimura
title	Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry
title_short	Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry
title_full	Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry
title_fullStr	Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry
title_full_unstemmed	Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry
title_sort	wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f0f59cf4b73b43128d3fd385a7a7e7ec
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Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry

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