Examining the effects of cigarette smoke on mouse lens through a multi OMIC approach

Abstract Here, we report a multi OMIC (transcriptome, proteome, and metabolome) approach to investigate molecular changes in lens fiber cells (FC) of mice exposed to cigarette smoke (CS). Pregnant mice were placed in a whole-body smoke chamber and a few days later pups were born, which were exposed...

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Autores principales:	Shahid Y. Khan, Muhammad Ali, Yura Jang, Taekyung Ryu, Andrew J. Schwab, Brian O. Ingram, Peter H. Cable, Chan Hyun Na, James T. Handa, S. Amer Riazuddin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f1063b2da7a34cb194c3953bea43b74f
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spelling	oai:doaj.org-article:f1063b2da7a34cb194c3953bea43b74f2021-12-02T18:13:52ZExamining the effects of cigarette smoke on mouse lens through a multi OMIC approach10.1038/s41598-021-95013-72045-2322https://doaj.org/article/f1063b2da7a34cb194c3953bea43b74f2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95013-7https://doaj.org/toc/2045-2322Abstract Here, we report a multi OMIC (transcriptome, proteome, and metabolome) approach to investigate molecular changes in lens fiber cells (FC) of mice exposed to cigarette smoke (CS). Pregnant mice were placed in a whole-body smoke chamber and a few days later pups were born, which were exposed to CS for 5 hours/day, 5 days/week for a total of 3½ months. We examined the mice exposed to CS for CS-related cataractogenesis after completion of the CS exposure but no cataracts were observed. Lenses of CS-exposed and age-matched, untreated control mice were extracted and lens FC were subjected to multi OMIC profiling. We identified 348 genes, 130 proteins, and 14 metabolites exhibiting significant (p < 0.05) differential levels in lens FC of mice exposed to CS, corresponding to 3.6%, 4.3%, and 5.0% of the total genes, protein, and metabolites, respectively identified in this study. Our multi OMIC approach confirmed that only a small fraction of the transcriptome, the proteome, and the metabolome was perturbed in the lens FC of mice exposed to CS, which suggests that exposure of CS had a minimal effect on the mouse lens. It is worth noting that while our results confirm that CS exposure does not have a substantial impact on the molecular landscape of the mouse lens FC, we cannot rule out that CS exposure for longer durations and/or in combination with other morbidities or environmental factors would have a more robust effect and/or result in cataractogenesis.Shahid Y. KhanMuhammad AliYura JangTaekyung RyuAndrew J. SchwabBrian O. IngramPeter H. CableChan Hyun NaJames T. HandaS. Amer RiazuddinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shahid Y. Khan Muhammad Ali Yura Jang Taekyung Ryu Andrew J. Schwab Brian O. Ingram Peter H. Cable Chan Hyun Na James T. Handa S. Amer Riazuddin Examining the effects of cigarette smoke on mouse lens through a multi OMIC approach
description	Abstract Here, we report a multi OMIC (transcriptome, proteome, and metabolome) approach to investigate molecular changes in lens fiber cells (FC) of mice exposed to cigarette smoke (CS). Pregnant mice were placed in a whole-body smoke chamber and a few days later pups were born, which were exposed to CS for 5 hours/day, 5 days/week for a total of 3½ months. We examined the mice exposed to CS for CS-related cataractogenesis after completion of the CS exposure but no cataracts were observed. Lenses of CS-exposed and age-matched, untreated control mice were extracted and lens FC were subjected to multi OMIC profiling. We identified 348 genes, 130 proteins, and 14 metabolites exhibiting significant (p < 0.05) differential levels in lens FC of mice exposed to CS, corresponding to 3.6%, 4.3%, and 5.0% of the total genes, protein, and metabolites, respectively identified in this study. Our multi OMIC approach confirmed that only a small fraction of the transcriptome, the proteome, and the metabolome was perturbed in the lens FC of mice exposed to CS, which suggests that exposure of CS had a minimal effect on the mouse lens. It is worth noting that while our results confirm that CS exposure does not have a substantial impact on the molecular landscape of the mouse lens FC, we cannot rule out that CS exposure for longer durations and/or in combination with other morbidities or environmental factors would have a more robust effect and/or result in cataractogenesis.
format	article
author	Shahid Y. Khan Muhammad Ali Yura Jang Taekyung Ryu Andrew J. Schwab Brian O. Ingram Peter H. Cable Chan Hyun Na James T. Handa S. Amer Riazuddin
author_facet	Shahid Y. Khan Muhammad Ali Yura Jang Taekyung Ryu Andrew J. Schwab Brian O. Ingram Peter H. Cable Chan Hyun Na James T. Handa S. Amer Riazuddin
author_sort	Shahid Y. Khan
title	Examining the effects of cigarette smoke on mouse lens through a multi OMIC approach
title_short	Examining the effects of cigarette smoke on mouse lens through a multi OMIC approach
title_full	Examining the effects of cigarette smoke on mouse lens through a multi OMIC approach
title_fullStr	Examining the effects of cigarette smoke on mouse lens through a multi OMIC approach
title_full_unstemmed	Examining the effects of cigarette smoke on mouse lens through a multi OMIC approach
title_sort	examining the effects of cigarette smoke on mouse lens through a multi omic approach
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f1063b2da7a34cb194c3953bea43b74f
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Examining the effects of cigarette smoke on mouse lens through a multi OMIC approach

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