Optimized CRISPR/Cas9-mediated in vivo genome engineering applicable to monitoring dynamics of endogenous proteins in the mouse neural tissues

Abstract To analyze the expression, localization, and functional dynamics of target proteins in situ, especially in living cells, it is important to develop a convenient, versatile, and efficient method to precisely introduce exogenous genes into the genome, which is applicable for labeling and engi...

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Bibliographic Details
Main Authors: Takahiko Matsuda, Izumi Oinuma
Format: article
Language:EN
Published: Nature Portfolio 2019
Subjects:
R
Q
Online Access:https://doaj.org/article/f107d8d68d0e403a88d8d8bf7f405aaf
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