Genome-wide identification and characterization of circular RNA in resected hepatocellular carcinoma and background liver tissue

Abstract Circular RNA (circRNA) is a type of non-coding RNA known to affect cancer-related micro RNAs and various transcription factors. circRNA has promise as a cancer-related biomarker because its circular structure affords high stability. We found using high-throughput sequencing that seven candi...

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Autores principales:	Yuki Sunagawa, Suguru Yamada, Fuminori Sonohara, Keisuke Kurimoto, Nobutake Tanaka, Yunosuke Suzuki, Yoshikuni Inokawa, Hideki Takami, Masamichi Hayashi, Mitsuro Kanda, Chie Tanaka, Goro Nakayama, Masahiko Koike, Yasuhiro Kodera
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f10be62b19494c76af33c843a3615951
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spelling	oai:doaj.org-article:f10be62b19494c76af33c843a36159512021-12-02T13:18:01ZGenome-wide identification and characterization of circular RNA in resected hepatocellular carcinoma and background liver tissue10.1038/s41598-021-85237-y2045-2322https://doaj.org/article/f10be62b19494c76af33c843a36159512021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85237-yhttps://doaj.org/toc/2045-2322Abstract Circular RNA (circRNA) is a type of non-coding RNA known to affect cancer-related micro RNAs and various transcription factors. circRNA has promise as a cancer-related biomarker because its circular structure affords high stability. We found using high-throughput sequencing that seven candidate circRNAs (hsa_circ_0041150, hsa_circ_0025624, hsa_circ_0001020, hsa_circ_0028129, hsa_circ_0008558, hsa_circ_0036683, hsa_circ_0058087) were downregulated in HCC. The expression of these circRNAs was examined by quantitative PCR in 233 sets of HCC and matched background normal liver tissues, and correlations between candidate circRNA expression and prognosis were evaluated. The results of quantitative PCR showed that expression of hsa_circ_0041150, hsa_circ_0001020 and hsa_circ_0008558 was significantly lower in HCC than in background normal liver tissues. Kaplan–Meier analysis revealed that low expression of hsa_circ_0001020, hsa_circ_0036683, and hsa_circ_0058087 was associated with poor recurrence-free (RFS) and overall survival (OS) in HCC. Additionally, multivariate analysis revealed that low hsa_circ_0036683 expression was a significant prognostic factor, independent from other clinicopathological features, for inferior RFS and OS. There was no significant association between the expression of these circRNAs and hepatitis B/C status or cirrhosis. This study therefore identified circRNAs as potential prognostic markers for patients who undergo curative surgery for HCC and highlighted hsa_circ_0036683 as the most useful biomarker.Yuki SunagawaSuguru YamadaFuminori SonoharaKeisuke KurimotoNobutake TanakaYunosuke SuzukiYoshikuni InokawaHideki TakamiMasamichi HayashiMitsuro KandaChie TanakaGoro NakayamaMasahiko KoikeYasuhiro KoderaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuki Sunagawa Suguru Yamada Fuminori Sonohara Keisuke Kurimoto Nobutake Tanaka Yunosuke Suzuki Yoshikuni Inokawa Hideki Takami Masamichi Hayashi Mitsuro Kanda Chie Tanaka Goro Nakayama Masahiko Koike Yasuhiro Kodera Genome-wide identification and characterization of circular RNA in resected hepatocellular carcinoma and background liver tissue
description	Abstract Circular RNA (circRNA) is a type of non-coding RNA known to affect cancer-related micro RNAs and various transcription factors. circRNA has promise as a cancer-related biomarker because its circular structure affords high stability. We found using high-throughput sequencing that seven candidate circRNAs (hsa_circ_0041150, hsa_circ_0025624, hsa_circ_0001020, hsa_circ_0028129, hsa_circ_0008558, hsa_circ_0036683, hsa_circ_0058087) were downregulated in HCC. The expression of these circRNAs was examined by quantitative PCR in 233 sets of HCC and matched background normal liver tissues, and correlations between candidate circRNA expression and prognosis were evaluated. The results of quantitative PCR showed that expression of hsa_circ_0041150, hsa_circ_0001020 and hsa_circ_0008558 was significantly lower in HCC than in background normal liver tissues. Kaplan–Meier analysis revealed that low expression of hsa_circ_0001020, hsa_circ_0036683, and hsa_circ_0058087 was associated with poor recurrence-free (RFS) and overall survival (OS) in HCC. Additionally, multivariate analysis revealed that low hsa_circ_0036683 expression was a significant prognostic factor, independent from other clinicopathological features, for inferior RFS and OS. There was no significant association between the expression of these circRNAs and hepatitis B/C status or cirrhosis. This study therefore identified circRNAs as potential prognostic markers for patients who undergo curative surgery for HCC and highlighted hsa_circ_0036683 as the most useful biomarker.
format	article
author	Yuki Sunagawa Suguru Yamada Fuminori Sonohara Keisuke Kurimoto Nobutake Tanaka Yunosuke Suzuki Yoshikuni Inokawa Hideki Takami Masamichi Hayashi Mitsuro Kanda Chie Tanaka Goro Nakayama Masahiko Koike Yasuhiro Kodera
author_facet	Yuki Sunagawa Suguru Yamada Fuminori Sonohara Keisuke Kurimoto Nobutake Tanaka Yunosuke Suzuki Yoshikuni Inokawa Hideki Takami Masamichi Hayashi Mitsuro Kanda Chie Tanaka Goro Nakayama Masahiko Koike Yasuhiro Kodera
author_sort	Yuki Sunagawa
title	Genome-wide identification and characterization of circular RNA in resected hepatocellular carcinoma and background liver tissue
title_short	Genome-wide identification and characterization of circular RNA in resected hepatocellular carcinoma and background liver tissue
title_full	Genome-wide identification and characterization of circular RNA in resected hepatocellular carcinoma and background liver tissue
title_fullStr	Genome-wide identification and characterization of circular RNA in resected hepatocellular carcinoma and background liver tissue
title_full_unstemmed	Genome-wide identification and characterization of circular RNA in resected hepatocellular carcinoma and background liver tissue
title_sort	genome-wide identification and characterization of circular rna in resected hepatocellular carcinoma and background liver tissue
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f10be62b19494c76af33c843a3615951
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Genome-wide identification and characterization of circular RNA in resected hepatocellular carcinoma and background liver tissue

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