Ex Vivo Vibration Spectroscopic Analysis of Colorectal Polyps for the Early Diagnosis of Colorectal Carcinoma

Colorectal cancer is one of the most common and often fatal cancers in humans, but it has the highest chance of a cure if detected at an early precancerous stage. Carcinogenesis in the colon begins as an uncontrolled growth forming polyps. Some of these polyps can finally be converted to colon cance...

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Autores principales:	Alla Synytsya, Aneta Vaňková, Michaela Miškovičová, Jaromír Petrtýl, Luboš Petruželka
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	colon polyps colorectal carcinoma early cancer diagnosis vibrational spectroscopy chemometrics Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f11098cb55654333b4fca4aa3380148c
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spelling	oai:doaj.org-article:f11098cb55654333b4fca4aa3380148c2021-11-25T17:21:08ZEx Vivo Vibration Spectroscopic Analysis of Colorectal Polyps for the Early Diagnosis of Colorectal Carcinoma10.3390/diagnostics111120482075-4418https://doaj.org/article/f11098cb55654333b4fca4aa3380148c2021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/2048https://doaj.org/toc/2075-4418Colorectal cancer is one of the most common and often fatal cancers in humans, but it has the highest chance of a cure if detected at an early precancerous stage. Carcinogenesis in the colon begins as an uncontrolled growth forming polyps. Some of these polyps can finally be converted to colon cancer. Early diagnosis of adenomatous polyps is the main approach for screening and preventing colorectal cancer, and vibration spectroscopy can be used for this purpose. This work is focused on evaluating FTIR and Raman spectroscopy as a tool in the ex vivo analysis of colorectal polyps, which could be important for the early diagnosis of colorectal carcinoma. Multivariate analyses (PCA and LDA) were used to assist the spectroscopic discrimination of normal colon tissue, as well as benign and malignant colon polyps. The spectra demonstrated evident differences in the characteristic bands of the main tissue constituents, i.e., proteins, nucleic acids, lipids, polysaccharides, etc. Suitable models for discriminating the three mentioned diagnostic groups were proposed based on multivariate analyses of the spectroscopic data. LDA classification was especially successful in the case of a combined set of 55 variables from the FTIR, FT Raman and dispersion Raman spectra. This model can be proposed for ex vivo colorectal cancer diagnostics in combination with the colonoscopic extraction of colon polyps for further testing. This pilot study is a precursor for the further evaluation of the diagnostic potential for the simultaneous in vivo application of colonoscopic Raman probes.Alla SynytsyaAneta VaňkováMichaela MiškovičováJaromír PetrtýlLuboš PetruželkaMDPI AGarticlecolon polypscolorectal carcinomaearly cancer diagnosisvibrational spectroscopychemometricsMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 2048, p 2048 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	colon polyps colorectal carcinoma early cancer diagnosis vibrational spectroscopy chemometrics Medicine (General) R5-920
spellingShingle	colon polyps colorectal carcinoma early cancer diagnosis vibrational spectroscopy chemometrics Medicine (General) R5-920 Alla Synytsya Aneta Vaňková Michaela Miškovičová Jaromír Petrtýl Luboš Petruželka Ex Vivo Vibration Spectroscopic Analysis of Colorectal Polyps for the Early Diagnosis of Colorectal Carcinoma
description	Colorectal cancer is one of the most common and often fatal cancers in humans, but it has the highest chance of a cure if detected at an early precancerous stage. Carcinogenesis in the colon begins as an uncontrolled growth forming polyps. Some of these polyps can finally be converted to colon cancer. Early diagnosis of adenomatous polyps is the main approach for screening and preventing colorectal cancer, and vibration spectroscopy can be used for this purpose. This work is focused on evaluating FTIR and Raman spectroscopy as a tool in the ex vivo analysis of colorectal polyps, which could be important for the early diagnosis of colorectal carcinoma. Multivariate analyses (PCA and LDA) were used to assist the spectroscopic discrimination of normal colon tissue, as well as benign and malignant colon polyps. The spectra demonstrated evident differences in the characteristic bands of the main tissue constituents, i.e., proteins, nucleic acids, lipids, polysaccharides, etc. Suitable models for discriminating the three mentioned diagnostic groups were proposed based on multivariate analyses of the spectroscopic data. LDA classification was especially successful in the case of a combined set of 55 variables from the FTIR, FT Raman and dispersion Raman spectra. This model can be proposed for ex vivo colorectal cancer diagnostics in combination with the colonoscopic extraction of colon polyps for further testing. This pilot study is a precursor for the further evaluation of the diagnostic potential for the simultaneous in vivo application of colonoscopic Raman probes.
format	article
author	Alla Synytsya Aneta Vaňková Michaela Miškovičová Jaromír Petrtýl Luboš Petruželka
author_facet	Alla Synytsya Aneta Vaňková Michaela Miškovičová Jaromír Petrtýl Luboš Petruželka
author_sort	Alla Synytsya
title	Ex Vivo Vibration Spectroscopic Analysis of Colorectal Polyps for the Early Diagnosis of Colorectal Carcinoma
title_short	Ex Vivo Vibration Spectroscopic Analysis of Colorectal Polyps for the Early Diagnosis of Colorectal Carcinoma
title_full	Ex Vivo Vibration Spectroscopic Analysis of Colorectal Polyps for the Early Diagnosis of Colorectal Carcinoma
title_fullStr	Ex Vivo Vibration Spectroscopic Analysis of Colorectal Polyps for the Early Diagnosis of Colorectal Carcinoma
title_full_unstemmed	Ex Vivo Vibration Spectroscopic Analysis of Colorectal Polyps for the Early Diagnosis of Colorectal Carcinoma
title_sort	ex vivo vibration spectroscopic analysis of colorectal polyps for the early diagnosis of colorectal carcinoma
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f11098cb55654333b4fca4aa3380148c
work_keys_str_mv	AT allasynytsya exvivovibrationspectroscopicanalysisofcolorectalpolypsfortheearlydiagnosisofcolorectalcarcinoma AT anetavankova exvivovibrationspectroscopicanalysisofcolorectalpolypsfortheearlydiagnosisofcolorectalcarcinoma AT michaelamiskovicova exvivovibrationspectroscopicanalysisofcolorectalpolypsfortheearlydiagnosisofcolorectalcarcinoma AT jaromirpetrtyl exvivovibrationspectroscopicanalysisofcolorectalpolypsfortheearlydiagnosisofcolorectalcarcinoma AT lubospetruzelka exvivovibrationspectroscopicanalysisofcolorectalpolypsfortheearlydiagnosisofcolorectalcarcinoma
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Ex Vivo Vibration Spectroscopic Analysis of Colorectal Polyps for the Early Diagnosis of Colorectal Carcinoma

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